FastQCFastQC Report
Wed 8 May 2024
EGAF00007894142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12110924
Total Bases920.4 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT214990.17751742146181415TruSeq Adapter, Index 2 (97% over 37bp)
TAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT199150.16443832031313219TruSeq Adapter, Index 2 (97% over 37bp)
CCTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT198550.16394289981507604TruSeq Adapter, Index 2 (97% over 37bp)
AAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTT196450.16220892807187956TruSeq Adapter, Index 2 (97% over 37bp)
AAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT188550.15568589151414047TruSeq Adapter, Index 2 (97% over 37bp)
AAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTT181860.15016195296081455TruSeq Adapter, Index 2 (97% over 37bp)
AAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT178250.1471811729641768TruSeq Adapter, Index 2 (97% over 37bp)
GTTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT166290.13730579103625784TruSeq Adapter, Index 2 (97% over 37bp)
CCTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT160260.1323268150307937TruSeq Adapter, Index 2 (97% over 37bp)
CCTGTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTT149410.12336796102427856TruSeq Adapter, Index 2 (97% over 37bp)
CCTGTCTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT139780.11541646203047762TruSeq Adapter, Index 2 (97% over 37bp)
TTAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT134940.11142007001282478TruSeq Adapter, Index 2 (97% over 37bp)
GTTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT133590.1103053738921985TruSeq Adapter, Index 2 (97% over 37bp)
TTAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT126780.10468235123926135TruSeq Adapter, Index 2 (97% over 37bp)
CTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTT123340.10184194038373952TruSeq Adapter, Index 2 (97% over 37bp)
GTTGTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTT121330.10018228171525145TruSeq Adapter, Index 2 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)