FastQCFastQC Report
Wed 31 May 2023
EGAF00007894143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12110924
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG310210.2561406545033228No Hit
CCTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG203270.16784020773311764Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG187090.15448036830220385Illumina Single End PCR Primer 1 (96% over 33bp)
TAAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG184990.15274639655900737Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATGG183980.15191243872061289Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG171690.14176457551876306Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATGG166980.1378755246090224Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG163270.1348121745293753Illumina Single End PCR Primer 1 (96% over 33bp)
TAAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG156750.1294286051171653Illumina Single End PCR Primer 1 (96% over 33bp)
AAAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG153360.12662947930314813Illumina Single End PCR Primer 1 (96% over 33bp)
AAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATGG143590.11856238219313407Illumina Single End PCR Primer 1 (96% over 33bp)
TTAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG129310.10677137433939805Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGACTAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG128930.10645760802396251Illumina Single End PCR Primer 1 (96% over 33bp)
AAAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG128660.10623466879983723Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)