FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007899145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007899145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66149952
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA1867700.28234336436102025No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC1774190.26820729968178963No Hit
ACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCT1688760.255292702253208No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA1612160.2437129508423528No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG1359450.20551035320479144No Hit
GTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAG1350110.20409840962545217No Hit
CTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTG1332240.2013969715352174No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT1170400.17693134531677362No Hit
CCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTG1156590.1748436642856521No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG1147080.17340602151910858No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC1101470.16651108076389837No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA1076130.16268039015357108No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT1061680.16049595924121002No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG1046760.1582404776348137No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA982890.1485851418304884No Hit
CACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGAC938730.14190940002496147No Hit
CTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTG922090.13939390311273392No Hit
CCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGA900080.13606661422823102No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT862130.1303296486141063No Hit
CTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGG855140.12927295850494344No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGA836380.12643697761110997No Hit
CTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTG798980.12078315642617549No Hit
CTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTT795660.12028126641724549No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG765110.11566297130495272No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG747970.11307188854800682No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG737550.11149667954407586No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT701120.10598949489789501No Hit
CGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGC690850.10443696164737958No Hit
GTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTG684420.10346492768430127No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG672140.10160853933801797No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT668510.10105978610536255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)