FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007899166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007899166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57164706
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1107170.19368069521778No Hit
GGCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAG1017110.1779262190205264No Hit
CTTTAATAGAAATTGGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGG937750.16404352713718146No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT913270.15976116451994No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT889490.1556012550821131No Hit
TGAAAATAAATGTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTC835100.14608664304159982No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG764220.13368738396030586No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT700550.12254939262698211No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG685690.11994988656112392No Hit
GGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCC666000.1165054535573051No Hit
CAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACA649040.11353858795320315No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC634280.1109565751986899No Hit
GACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCA625750.10946439574096645No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA588450.10293939060930359No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)