FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007899172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007899172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57710849
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAATAGAAATTGGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGG2728770.472834839078524No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2218450.38440779133226755No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC2145260.3717256005019091No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT2137120.37031512047240894No Hit
CTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGA2076060.3597347874747086No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1787770.3097805752259857No Hit
CAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACA1761140.30516619154225233No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1618400.2804325405089778No Hit
GAAAATAAATGTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCA1473520.2553280753156135No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC1430720.24791179211381903No Hit
TGAAAATAAATGTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTC1393260.2414208115357998No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1383460.23972269061576273No Hit
CTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACT1320930.22888763948005686No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG1240890.21501849678212148No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC1182810.2049545311662284No Hit
GTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCAT1131600.19608098297081022No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1044450.18097983621762348No Hit
CAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGG1032650.17893516000778295No Hit
GTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCA964360.16710202963744306No Hit
CAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCA917780.15903075693791993No Hit
CTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGT911180.15788712448156847No Hit
AAAATAAATGTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCAT904010.15664472376762298No Hit
GTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAA880820.1526264151823516No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT841150.1457524910090995No Hit
GTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCC833840.1444858314248678No Hit
CTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTC823170.1426369589537662No Hit
CTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAA820570.1421864370770217No Hit
CAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTA817120.14158862920211068No Hit
GACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCA774840.13426245037566506No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA769240.1332920955642153No Hit
GCACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCATCA755590.13092685571130655No Hit
GTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCT730840.12663823399998844No Hit
CCTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAG728900.12630207536887908No Hit
AGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACAC696200.12063589638059213No Hit
CTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTT685420.11876796336855139No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA675190.11699533306120657No Hit
GGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCT670210.11613241038959589No Hit
GCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACC664760.11518804722488142No Hit
GTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCAC636340.11026349655677392No Hit
CTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGG612120.10606671199725375No Hit
AATAAATGTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCATAA587020.10171744311022006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)