FastQCFastQC Report
Sun 19 Mar 2023
EGAF00007899791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007899791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398655574
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC179534194.503491276908623TruSeq Adapter, Index 6 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCCCGTATGCC4955250.12429902710955197TruSeq Adapter, Index 6 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATGCC4686490.11755736795492541TruSeq Adapter, Index 6 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)