FastQCFastQC Report
Tue 21 Feb 2023
EGAF00007902454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007902454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85627
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC44525.199294615016291No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC17622.0577621544606255No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG17572.0519228747941654No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA15301.7868195779368656No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG13241.5462412556786995No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG12981.5158770014131056No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG12601.4714984759480072No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG12501.4598199166150863No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC12101.4131056792834036No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC11661.3617200182185525No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG11381.3290200520863746No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG11361.3266843402197905No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG10361.2098987468905835No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC9671.1293166874934308No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG9211.0755953145619956No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC9021.0534060518294464No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA5920.6913707125089049No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA4660.5442208649141042No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG4330.5056816191154659No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4250.4963387716491294No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3930.4589673817837831No Hit
GACTACCTGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3890.45429595805061485No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3740.43677811905123387No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3660.42743527158489725No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3630.4239317037850211No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3530.4122531444521004No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG3500.40874957665222417No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA3400.3970710173193035No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3260.3807210342532145No Hit
GACTACATGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3200.3737138986534621No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC3190.37254604272017006No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2920.3410139325212842No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG2890.33751036472140794No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2760.32232823758861107No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG2740.3199925257220269No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2680.3129853901222745No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG2670.31181753418898245No Hit
CCTACGGGGGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT2630.3071461104558142No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG2610.30481039858923004No Hit
GACTACTTGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2580.30130683078935383No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG2420.28262113585668075No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG2410.28145327992338864No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC2350.27444614432363623No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG2290.2674390087238838No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG2270.26510329685729966No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2180.2545925934576711No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG2130.2487533137912107No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG2100.24524974599133448No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG2070.2417461781914583No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG2060.24057832225816622No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG2010.2347390425917059No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC2010.2347390425917059No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1920.22422833919207727No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1840.2148854917257407No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG1830.21371763579244862No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTCAG1750.20437478832611208No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1730.20203907645952793No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG1690.19736765272635967No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1680.19619979679306762No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGCCGAAAGTCTGAC1670.19503194085977554No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1650.1926962289931914No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTCAG1620.18919266119331518No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTCAG1610.18802480526002313No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGCCTGAC1600.18685694932673105No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG1600.18685694932673105No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTCAG1580.18452123746014693No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1570.18335338152685485No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA1550.18101766966027072No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTAAG1550.18101766966027072No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1530.17868195779368656No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTAAG1520.1775141018603945No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1510.17634624592710244No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1480.17284267812722623No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1460.1705069662606421No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA1460.1705069662606421No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTCAG1450.16933911032735002No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG1410.16466768659418174No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1400.1634998306608897No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTAAG1390.16233197472759758No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1370.15999626286101346No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTCAG1350.15766055099442933No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1330.15532483912784517No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1330.15532483912784517No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1320.15415698319455312No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTAAG1320.15415698319455312No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTAAG1300.15182127132796897No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTCAG1290.15065341539467692No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTAAG1270.14831770352809276No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1230.1436462797949245No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1200.1401427119950483No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1180.13780700012846417No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTCAG1160.13547128826188No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1120.13079986452871176No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1110.12963200859541968No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1110.12963200859541968No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1090.12729629672883555No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTCAG1090.12729629672883555No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1080.12612844079554345No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1080.12612844079554345No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1070.12496058486225138No Hit
CCTACGGGCGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT1050.12262487299566724No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1050.12262487299566724No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGCAAGTCTGAC1040.12145701706237517No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1030.12028916112908311No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTAAG1030.12028916112908311No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1010.11795344926249897No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTAAG990.11561773739591484No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG980.11444988146262276No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG980.11444988146262276No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG970.1132820255293307No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC960.11211416959603863No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT950.11094631366274656No Hit
GACTACTGGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG950.11094631366274656No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG950.11094631366274656No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT940.10977845772945449No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT940.10977845772945449No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG940.10977845772945449No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA940.10977845772945449No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTTCACAATGGGCGAAAGCCTGAT930.10861060179616243No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG930.10861060179616243No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC930.10861060179616243No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG930.10861060179616243No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG930.10861060179616243No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA920.10744274586287035No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG910.10627488992957829No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT910.10627488992957829No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG910.10627488992957829No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTAAG910.10627488992957829No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGCCGCAAGTCTGAC900.10510703399628622No Hit
GACTACCTGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTCAG900.10510703399628622No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA900.10510703399628622No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA900.10510703399628622No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG890.10393917806299416No Hit
GACTACATGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTCAG890.10393917806299416No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG880.10277132212970208No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCAG870.10160346619641002No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG870.10160346619641002No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)