FastQCFastQC Report
Tue 21 Feb 2023
EGAF00007902514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007902514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61310
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT44137.197847007013538No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT15612.5460773120208775No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC14572.3764475615723373No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT10561.7223943891697928No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA10411.6979285597781766No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT10041.6375795139455227No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA7981.301582123633991No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA6771.1042244332082858No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA6731.0977002120371881No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA6251.0194095579840157No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA5920.9655847333224596No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC5750.9378567933452944No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC5510.8987114663187082No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA5440.8872940792692873No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC5390.8791388028054151No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA4960.8090034252161149No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC4810.7845375958244984No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA4410.7192953841135215No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA4210.686674278258033No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3940.6426357853531235No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT3630.5920730712771163No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3470.5659761865927254No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3430.5594519654216278No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3330.5431414124938835No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3290.5366171913227858No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2910.4746370901973577No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2900.47300603490458326No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2880.4697439243190344No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2660.4338607078779971No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2560.4175501549502528No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2470.402870657315283No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2420.39471538085141084No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2380.38819115968031315No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2370.38656010438753874No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2370.38656010438753874No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2330.38003588321644105No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATTAA2320.37840482792366664No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2300.37514271733811777No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2290.37351166204534336No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2270.3702495514597945No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2240.3653563855814712No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2140.34904583265372696No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2100.3425216114826293No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2050.3343663350187571No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2030.3311042244332083No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2020.3294731691404339No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1980.32294894796933615No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1980.32294894796933615No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1890.3082694503343663No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATTAA1830.29848311857771975No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1830.29848311857771975No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATTAA1820.29685206328494534No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1780.29032784211384766No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATTAA1750.2854346762355244No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1750.2854346762355244No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1750.2854346762355244No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1710.2789104550644267No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1700.2772793997716523No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1670.272386233893329No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGACAATGGTCGGAAGACTGAT1630.2658620127222313No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1620.26423095742945685No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT1620.26423095742945685No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1610.26259990213668244No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1600.26096884684390803No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1550.2528135703800359No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1530.249551459794487No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATTAA1530.249551459794487No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1500.24465829391616375No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1490.24302723862338932No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1460.23813407274506604No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1440.2348719621595172No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATTAA1420.23160985157396835No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1400.2283477409884195No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1400.2283477409884195No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATTAA1390.22671668569564507No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1380.22508563040287066No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT1370.22345457511009623No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1360.22182351981732182No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATTAA1350.22019246452454738No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1350.22019246452454738No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATTAA1330.21693035393899854No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1280.2087750774751264No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1260.20551296688957757No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1260.20551296688957757No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1240.20225085630402873No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGTACAATGGGCGCAAGCCTGAT1230.2006198010112543No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATGAA1230.2006198010112543No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1220.19898874571847988No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1200.19572663513293098No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1150.18757135866905889No Hit
GACTACATGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATTAA1080.1761539716196379No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATCAA1070.17452291632686348No Hit
GACTACTTGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATTAA1050.17126080574131464No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATCAA1040.1696297504485402No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATCAA1020.16636763986299136No Hit
GACTACCTGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1010.16473658457021695No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT980.15984341869189367No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG970.15821236339911923No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGTACAATGGGCGCAAGCCTGAT970.15821236339911923No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATCAA970.15821236339911923No Hit
GACTACCTGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATTAA950.15495025281357036No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT940.15331919752079595No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG930.15168814222802152No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG920.1500570869352471No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC910.14842603164247267No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG900.14679497634969826No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC890.14516392105692383No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT880.1435328657641494No Hit
GACTACCTGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA880.1435328657641494No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATGAA860.14027075517860055No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGCCAATGGGCGCAAGCCTGAT850.13863969988582614No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATGAA830.1353775893002773No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATCAA830.1353775893002773No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGGCAATGGGCGCAAGCCTGAT830.1353775893002773No Hit
GACTACTTGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG810.13211547871472842No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATGAA790.12885336812917958No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATGAA790.12885336812917958No Hit
GACTACCTGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG740.12069809166530746No Hit
GACTACATGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG740.12069809166530746No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATGAA710.11580492578698418No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATGAA710.11580492578698418No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGTGCAATGGGCGCAAGCCTGAT700.11417387049420975No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATGAA690.11254281520143533No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATCAA690.11254281520143533No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATGAA690.11254281520143533No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATTAA680.11091175990866091No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT670.10928070461588649No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATTAA660.10764964932311205No Hit
GACTACATGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA650.10601859403033763No Hit
GACTACTTGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA640.1043875387375632No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATAAA620.10112542815201436No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)