FastQCFastQC Report
Fri 24 Feb 2023
EGAF00007902518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007902518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54334
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC39427.255125703979093No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC16042.9521110170427356No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG12752.3465969742702546No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC12112.2288070085029634No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC10581.9472153715905327No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG9621.770530422939596No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG9511.7502852725733427No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG9181.689549821474583No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG8741.6085692200095707No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG8501.5643979828468362No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG8061.4834173813818237No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG7441.3693083520447602No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG6841.2588802591379247No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC6261.152133102661317No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC5601.030662200463798No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA5481.0085765818824308No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3610.6644090256561269No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3510.6460043435049877No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT3440.6331210659991902No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2810.517171568447013No Hit
GACTACCTGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2600.47852173592962055No Hit
GACTACATGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2500.4601170537784812No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2280.4196267530459749No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC2280.4196267530459749No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2270.417786284830861No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2220.40858394375529133No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG2210.4067434755401774No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2150.3957006662494939No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2040.3754555158832407No Hit
GACTACTTGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2040.3754555158832407No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1980.36441270659255715No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1970.36257223837744323No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG1950.3588913019472154No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1950.3588913019472154No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1940.35705083373210145No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1920.35336989730187357No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1910.3515294290867597No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1850.34048661979607614No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1850.34048661979607614No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1840.3386461515809622No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1800.3312842787205065No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGCCGAAAGTCTGAC1710.31472006478448117No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1710.31472006478448117No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1690.31103912835425335No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1590.2926344462031141No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1580.2907939779880001No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1580.2907939779880001No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1560.2871130415577723No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1530.2815916369124305No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1520.2797511686973166No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1510.2779107004822027No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1480.2723892958368609No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1460.26870835940663307No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1450.2668678911915191No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG1440.2650274229764052No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1430.2631869547612913No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1390.2558250819008356No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1370.2521441454706077No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1350.24846320904037986No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1320.24294180439503812No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1220.22453712224389885No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1210.2226966540287849No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1190.21901571759855706No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1130.20797290830787352No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG1120.2061324400927596No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG1100.20245150366253176No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1060.19508963080207606No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGCAAGTCTGAC1060.19508963080207606No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG1010.18588728972650642No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG1010.18588728972650642No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1000.1840468215113925No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG960.17668494865093679No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG940.17300401222070894No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT930.17116354400559503No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT930.17116354400559503No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG900.16564213936025324No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG880.1619612029300254No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG880.1619612029300254No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC870.16012073471491148No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC830.15275886185445578No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTCAG810.1490779254242279No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC800.147237457209114No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGCCGCAAGTCTGAC790.14539698899400005No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG790.14539698899400005No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG790.14539698899400005No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTCAG790.14539698899400005No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT770.1417160525637722No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG760.1398755843486583No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTCAG740.13619464791843044No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC730.13435417970331653No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT720.1325137114882026No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC700.12883277505797475No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG690.12699230684286084No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG670.12331137041263299No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG650.11963043398240514No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCGGCAATGGACGAAAGTCTGAC650.11963043398240514No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTCAG640.1177899657672912No Hit
GACTACCTGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG640.1177899657672912No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTCAG640.1177899657672912No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT630.11594949755217727No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC620.11410902933706335No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGCCGAAAGTCTGAC620.11410902933706335No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG620.11410902933706335No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTCAG610.11226856112194943No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTCAG610.11226856112194943No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC600.1104280929068355No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC600.1104280929068355No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG590.10858762469172158No Hit
GACTACATGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG580.10674715647660765No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG580.10674715647660765No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTCAG570.10490668826149373No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG570.10490668826149373No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG560.1030662200463798No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG560.1030662200463798No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT550.10122575183126588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)