FastQCFastQC Report
Tue 21 Feb 2023
EGAF00007902536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007902536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70659
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC28103.9768465446722994No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG12851.818593526656194No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC10561.4945017619836114No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG10301.4577053170862877No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10061.4237393679502965No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG10001.4152478806662987No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG9581.3558074696783142No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG8891.2581553659123395No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG8711.2326809040603461No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG8441.194469211282356No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG7761.0982323553970477No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC7741.0954018596357151No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC6900.9765210376597462No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC6880.9736905418984134No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG6870.9722752940177471No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4600.6510140251064974No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4310.6099718365671748No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4050.573175391669851No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4040.5717601437891847No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3910.5533619213405228No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3680.5208112200851979No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA3440.4868452709492067No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3180.450048826051883No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3130.4429725866485515No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2940.4160828769158918No Hit
GACTACCTGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2910.41183713327389293No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2790.39485415870589735No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2760.39060841506389843No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2740.38777791930256583No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2740.38777791930256583No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2710.38353217566056697No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2710.38353217566056697No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2680.37928643201856804No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2670.3778711841379017No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2610.369379696853904No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2570.36371870533123873No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2560.36230345745057246No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2550.3608882095699062No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2510.35522721804724094No Hit
GACTACATGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2480.3509814744052421No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2460.3481509786439095No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2440.3453204828825769No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2380.3368289955985791No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2320.3283375083145813No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2300.3255070125532487No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2280.3226765167919161No Hit
GACTACTTGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2270.3212612689112498No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2140.3028630464625879No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2120.30003255070125534No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2100.29720205493992274No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2090.2957868070592564No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT2080.29437155917859015No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2050.2901258155365912No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2010.28446482401392603No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1980.2802190803719271No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1960.27738858461059457No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1950.27597333672992824No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG1930.27314284096859565No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA1800.2547446185199338No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1620.22927015666794037No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1560.22077866938394258No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG1520.2151176778612774No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1510.2137024299806111No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC1480.2094566863386122No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1470.2080414384579459No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1460.2066261905772796No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1440.20379569481594703No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG1440.20379569481594703No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1410.1995499511739481No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG1400.1981347032932818No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1340.18964321600928402No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1340.18964321600928402No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG1320.18681272024795142No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1320.18681272024795142No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1300.18398222448661883No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1280.18115172872528623No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1270.17973648084461993No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG1270.17973648084461993No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1230.17407548932195474No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1220.17266024144128844No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1220.17266024144128844No Hit
GACTACCTGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1210.17124499356062214No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1200.16982974567995585No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1190.16841449779928955No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG1180.16699924991862325No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG1180.16699924991862325No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1180.16699924991862325No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1180.16699924991862325No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1140.16133825839595806No Hit
GACTACATGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1140.16133825839595806No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1140.16133825839595806No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1110.15709251475395916No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1100.15567726687329286No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1090.15426201899262657No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1070.15143152323129394No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG1060.15001627535062767No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG1060.15001627535062767No Hit
GACTACTTGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1030.14577053170862878No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGCCGAAAGTCTGAC1020.14435528382796245No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTAAG1020.14435528382796245No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG1020.14435528382796245No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1010.14294003594729618No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT980.13869429230529728No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTAAG980.13869429230529728No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG980.13869429230529728No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG960.1358637965439647No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT960.1358637965439647No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG960.1358637965439647No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTAAG920.13020280502129947No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG910.12878755714063317No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG910.12878755714063317No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA900.1273723092599669No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC900.1273723092599669No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG900.1273723092599669No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG890.12595706137930057No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG880.12454181349863429No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA880.12454181349863429No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT880.12454181349863429No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG880.12454181349863429No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG870.12312656561796799No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCACAATGGGCGCAAGCCTGAT870.12312656561796799No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG870.12312656561796799No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG870.12312656561796799No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG860.12171131773730168No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTAAG850.1202960698566354No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG840.11888082197596908No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG840.11888082197596908No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG840.11888082197596908No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG830.1174655740953028No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGCGCTTAAG820.1160503262146365No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG820.1160503262146365No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG800.1132198304533039No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGCGCTTAAG800.1132198304533039No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTAAG790.11180458257263759No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA790.11180458257263759No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGCGCTTAAG790.11180458257263759No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT780.11038933469197129No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC780.11038933469197129No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG780.11038933469197129No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTAAG780.11038933469197129No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG770.10897408681130501No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC770.10897408681130501No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG770.10897408681130501No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG760.1075588389306387No Hit
CCTACGGGGGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT750.10614359104997241No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG750.10614359104997241No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG750.10614359104997241No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG740.1047283431693061No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG740.1047283431693061No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT740.1047283431693061No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG740.1047283431693061No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG730.1033130952886398No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGCCGCAAGTCTGAC730.1033130952886398No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT730.1033130952886398No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC730.1033130952886398No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTAAG720.10189784740797352No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG710.1004825995273072No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC710.1004825995273072No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG710.1004825995273072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)