FastQCFastQC Report
Fri 24 Feb 2023
EGAF00007902554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007902554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48545
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT11172.3009578741373984No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC10492.160881656195283No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGACAATGGTCGGAAGACTGAT5691.1721083530744671No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG5431.1185497991554227No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG4740.9764136368318056No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG4490.924915027294263No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4050.8342774745081882No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG4030.830157585745185No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG3970.8177979194561747No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3610.7436399217221135No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC3540.7292203110516016No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3520.7251004222885983No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3360.692141312184571No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG3290.6777217015140591No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG3280.6756617571325574No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3210.6612421464620455No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG3180.6550623133175404No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3020.6221032032135133No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2890.5953239262539911No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG2840.5850242043464826No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2790.5747244824389741No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2760.568544649294469No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2670.5500051498609538No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2660.547945205479452No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2650.5458852610979503No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG2640.5438253167164486No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2630.5417653723349469No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG2630.5417653723349469No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG2590.5335255948089401No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2590.5335255948089401No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2580.5314656504274384No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG2560.527345761664435No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG2540.5232258729014316No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG2530.5211659285199299No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG2490.5129261509939231No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2490.5129261509939231No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG2480.5108662066124214No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG2310.4758471521268926No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2310.4758471521268926No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG2230.45936759707487895No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG2210.4552477083118756No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGACAATGGTCGGAAGACTGAT2160.4449479864043671No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG2150.44288804202286536No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG2070.42640848697085176No Hit
CCTACGGGGGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT2050.42228859820784836No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG2050.42228859820784836No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG2040.4202286538263467No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2030.41816870944484497No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG2020.41610876506334327No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG2010.4140488206818416No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG1930.397569265629828No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG1880.3872695437223195No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1860.38314965495931613No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1820.3749098774333093No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1780.3666700999073025No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1730.356370377999794No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1730.356370377999794No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG1650.3398908229477804No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1650.3398908229477804No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1610.33165104542177365No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1600.3295911010402719No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGACAATGGTCGGAAGACTGAT1570.3234112678957668No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1530.31517149036976No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1510.31105160160675666No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1490.3069317128437532No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG1430.294572046554743No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1420.2925121021732413No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG1410.29045215779173966No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1400.2883922134102379No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGACAATGGTCGGAAGACTGAT1340.2760325471212277No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1340.2760325471212277No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1320.2719126583582243No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGTCGGAAGACTGAA1290.2657328252137192No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC1280.2636728808322175No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1260.2595529920692142No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1240.2554331033062107No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1230.253373158924709No Hit
GACTACATGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG1190.24513338139870222No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1160.23895354825419715No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG1130.23277371510969205No Hit
GACTACTTGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG1130.23277371510969205No Hit
GACTACCTGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG1110.22865382634668863No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1060.21835410443918013No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1060.21835410443918013No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1050.21629416005767843No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1040.21423421567617673No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG1030.21217427129467506No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT990.20393449376866823No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA980.20187454938716656No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT970.19981460500566486No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG950.19569471624266144No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG900.18539499433515294No Hit
CCTACGGGCGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT880.18127510557214957No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG880.18127510557214957No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG860.17715521680914614No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA850.17509527242764444No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG840.17303532804614274No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC830.17097538366464107No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGCAACCCTGAC820.16891543928313935No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG810.16685549490163765No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC810.16685549490163765No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG800.16479555052013595No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG790.16273560613863425No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT790.16273560613863425No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG790.16273560613863425No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT770.15861571737563085No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT770.15861571737563085No Hit
GACTACATGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG760.15655577299412915No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG760.15655577299412915No Hit
GACTACCTGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG750.15449582861262745No Hit
GACTACTGGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG750.15449582861262745No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT750.15449582861262745No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC750.15449582861262745No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG740.15243588423112575No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG730.15037593984962408No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA730.15037593984962408No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGAGGAAACTCTGAT720.14831599546812235No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCTCTCAG720.14831599546812235No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG710.14625605108662065No Hit
GACTACCTGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG690.14213616232361725No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG690.14213616232361725No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCTCTCAG680.14007621794211558No Hit
GACTACTTGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG680.14007621794211558No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA670.13801627356061386No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA670.13801627356061386No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC670.13801627356061386No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT660.13595632917911216No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG660.13595632917911216No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCTCTCAG640.13183644041610876No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC640.13183644041610876No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT630.1297764960346071No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG630.1297764960346071No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG630.1297764960346071No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT600.12359666289010196No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA590.12153671850860027No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG580.11947677412709858No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCTCTCAG580.11947677412709858No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG570.11741682974559686No Hit
GACTACCGGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG570.11741682974559686No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA570.11741682974559686No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG570.11741682974559686No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC560.11535688536409518No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG560.11535688536409518No Hit
CCTACGGGAGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT560.11535688536409518No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC560.11535688536409518No Hit
GACTACATGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG550.11329694098259346No Hit
GACTACAGGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG540.11123699660109178No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT540.11123699660109178No Hit
GACTACATGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG540.11123699660109178No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG530.10917705221959007No Hit
GACTACCTGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG530.10917705221959007No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG530.10917705221959007No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG520.10711710783808837No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG520.10711710783808837No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC510.10505716345658668No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG510.10505716345658668No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG510.10505716345658668No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG500.10299721907508497No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC500.10299721907508497No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC500.10299721907508497No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGTCGGAAGACTGAA500.10299721907508497No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG500.10299721907508497No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG500.10299721907508497No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT490.10093727469358328No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA490.10093727469358328No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG490.10093727469358328No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCTCTCAG490.10093727469358328No Hit
CCTACGGGGGGCAGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT490.10093727469358328No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG490.10093727469358328No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)