FastQCFastQC Report
Tue 21 Feb 2023
EGAF00007902594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007902594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65135
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT16712.565441007139019No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC16022.459507177400783No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC7411.137637215014969No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA7151.0977201197512858No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG6300.967221923696937No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT6030.925769555538497No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC5500.8444000921163738No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5240.8044829968526906No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA5090.7814539034313349No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG5070.7783833576418209No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG5050.7753128118523068No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA5020.7707069931680357No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4680.7185077147462962No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4550.6985491671144546No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG4390.6739848007983419No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4350.6678437092193137No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC4320.6632378905350426No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4290.6586320718507714No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA4250.6524909802717433No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4250.6524909802717433No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC4200.6448146157979581No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA4110.6309971597451447No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4050.6217855223766025No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3990.6125738850080602No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA3900.5987564289552468No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3820.5864742457971904No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA3590.5511629692177784No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3470.5327396944806939No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3420.5250633300069087No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA3270.502034236585553No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3220.49435787211176785No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3160.48514623474322555No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA3090.47439932447992633No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2800.42987641053197206No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG2780.426805864742458No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2660.40838259000537347No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2610.40070622553158824No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2530.3884240423735319No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2340.3592538573731481No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA2210.3392953097413065No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG2170.33315421816227836No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2080.31933676210946493No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1980.30398403316189454No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1970.30244876026713746No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1950.2993782144776234No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1890.2901665771090811No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1840.2824902126352959No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1830.28095493974053887No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1810.27788439395102477No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1790.2748138481615107No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATGAA1750.2686727565824825No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATGAA1690.25946111921394027No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1680.2579258463191832No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATGAA1670.25639057342442617No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1650.25332002763491207No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1650.25332002763491207No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1640.25178475474015505No Hit
GACTACCTGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1630.250249481845398No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1600.24564366316112687No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1600.24564366316112687No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1600.24564366316112687No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1590.24410839026636982No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1560.2395025715820987No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1540.23643202579258465No Hit
CCTACGGGGGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT1530.2348967528978276No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1530.2348967528978276No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1470.22568511552928536No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1460.2241498426345283No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1450.22261456973977126No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1430.21954402395025718No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1430.21954402395025718No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1370.2103323865817149No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1360.20879711368695786No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATGAA1360.20879711368695786No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1340.20572656789744379No Hit
GACTACATGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1330.20419129500268673No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1320.20265602210792968No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1310.20112074921317266No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1310.20112074921317266No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1290.19805020342365856No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1280.1965149305289015No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATGAA1280.1965149305289015No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1280.1965149305289015No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATGAA1250.1919091118446304No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1250.1919091118446304No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1250.1919091118446304No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1240.19037383894987334No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATGAA1230.1888385660551163No Hit
GACTACTTGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1220.18730329316035926No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATGAA1210.1857680202656022No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1200.18423274737084516No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1200.18423274737084516No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1190.18269747447608814No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC1180.1811622015813311No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1170.17962692868657404No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1150.17655638289705997No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1130.17348583710754586No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATGAA1120.17195056421278881No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1120.17195056421278881No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1100.16888001842327474No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1100.16888001842327474No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1090.1673447455285177No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1090.1673447455285177No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1080.16580947263376064No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATTAA1070.16427419973900362No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATTAA1070.16427419973900362No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1070.16427419973900362No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1040.15966838105473247No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1040.15966838105473247No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1040.15966838105473247No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1030.15813310815997544No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1030.15813310815997544No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG1030.15813310815997544No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1020.1565978352652184No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1010.15506256237046134No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1010.15506256237046134No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATTAA1010.15506256237046134No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATTAA1010.15506256237046134No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1010.15506256237046134No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1000.1535272894757043No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA960.14738619789667612No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG950.1458509250019191No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT940.14431565210716205No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATCAA910.13970983342289092No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATTAA910.13970983342289092No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTTCACAATGGGCGAAAGCCTGAT890.13663928763337682No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG890.13663928763337682No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATTAA850.13049819605434865No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG850.13049819605434865No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATCAA820.12589237737007752No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG820.12589237737007752No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC820.12589237737007752No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG800.12282183158056344No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG790.12128655868580639No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG780.11975128579104935No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATTAA770.11821601289629233No Hit
GACTACATGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATTAA760.11668074000153528No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATCAA720.1105396484225071No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATCAA720.1105396484225071No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG720.1105396484225071No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATTAA710.10900437552775007No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT710.10900437552775007No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATTAA700.10746910263299302No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATCAA700.10746910263299302No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG700.10746910263299302No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA690.10593382973823598No Hit
GACTACCTGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATTAA680.10439855684347893No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATTAA680.10439855684347893No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA680.10439855684347893No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCACAATGGACGAAAGTCTGAT680.10439855684347893No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG670.10286328394872189No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA670.10286328394872189No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG670.10286328394872189No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATTAA660.10132801105396484No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGCAACCCTGAC660.10132801105396484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)