FastQCFastQC Report
Tue 21 Feb 2023
EGAF00007902641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007902641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73587
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC25343.443543017109No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC19602.6635139358854145No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC14912.026173101227119No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG12021.6334406892521778No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC11311.5369562558604102No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT10661.4486254365580877No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10561.4350360797423458No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC10281.3969858806582685No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG9751.3249622895348363No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC9181.2475029556851074No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG8891.2080938209194558No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC8681.1795561716063978No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC6820.9267941348335983No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC6100.8289507657602566No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4990.6781089051055214No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4960.6740320980607988No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4920.668596355334502No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4870.661801676926631No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4830.6563659342003343No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4750.6454944487477408No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4640.6305461562504246No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4490.6101621210268118No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4460.6060853139820892No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG4460.6060853139820892No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4200.5707529862611602No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4180.568035114898012No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4170.5666761792164376No Hit
CCTACGGGGGGCTGCAGTGGGGAATGTTGGACAATGGGCGCAAGCCTGAT3800.5163955589981926No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3610.4905757810482829No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3360.45660238900892824No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3340.45388451764577986No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3200.4348594181037412No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA3140.426705804014296No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3130.42534686833272184No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA3110.42262899696957346No Hit
CCTACGGGGGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT2970.4036038974275348No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2850.3872966692486445No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2800.3805019908407735No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2370.3220677565330833No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2360.3207088208515091No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2180.29624797858317364No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2110.2867354288121543No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2070.2812996860858576No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2030.2758639433595608No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2020.2745050076779866No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2020.2745050076779866No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1890.25683884381752214No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1860.25276203677279957No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1820.24732629404650278No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT1800.2446084226833544No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1770.24053161563863182No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA1760.23917267995705763No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1760.23917267995705763No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1750.23781374427548344No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1750.23781374427548344No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1720.23373693723076086No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1700.23101906586761248No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1700.23101906586761248No Hit
CCTACGGGCGGCTGCAGTGGGGAATGTTGGACAATGGGCGCAAGCCTGAT1690.2296601301860383No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1670.22694225882288993No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1670.22694225882288993No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1610.21878864473344478No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1580.21471183768872218No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1570.213352902007148No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1560.2119939663255738No Hit
CCTACGGGAGGCTGCAGTGGGGAATGTTGGACAATGGGCGCAAGCCTGAT1540.2092760949624254No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1540.2092760949624254No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1480.2011224808729803No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1470.1997635451914061No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1440.19568673814668353No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1400.19025099542038676No Hit
CCTACGGGCGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT1390.18889205973881257No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1390.18889205973881257No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1340.1820973813309416No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1340.1820973813309416No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1330.18073844564936742No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1310.17802057428621904No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1260.17122589587834808No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1250.1698669601967739No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1240.1685080245151997No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1210.16443121747047712No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1200.16307228178890293No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATAGCCTGAA1160.15763653906260616No Hit
CNTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1160.15763653906260616No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA1150.15627760338103197No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1150.15627760338103197No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1130.1535597320178836No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCGGCAATGGACGAAAGTCTGAC1130.1535597320178836No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGAGGGAAATCTGAA1120.1522007963363094No Hit
CCTACGGGAGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT1090.14812398929158682No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGACAGCCTGAA1080.14676505361001263No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC1040.1413293108837159No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1040.1413293108837159No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGATGGAAATCTGAA1010.1372525038389933No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1000.1358935681574191No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA980.13317569679427071No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC940.12773995406797398No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG910.12366314702325139No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT890.120945275660103No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT880.11958633997852881No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCGGCAATGGACGAAAGTCTGAC850.11550953293380624No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA830.11279166157065787No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT820.11143272588908368No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT820.11143272588908368No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG800.1087148545259353No Hit
CCTACGGGCGGCCGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC800.1087148545259353No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA800.1087148545259353No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG760.10327911179963851No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA760.10327911179963851No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG760.10327911179963851No Hit
GNCTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG750.10192017611806432No Hit
CNTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC750.10192017611806432No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT740.10056124043649015No Hit
GACTACTGGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG740.10056124043649015No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)