FastQCFastQC Report
Tue 21 Feb 2023
EGAF00007902648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007902648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91640
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC19692.148625054561327No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG15121.6499345264076821No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT13711.496071584460934No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG12881.4054997817546921No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG11441.248363160192056No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG11061.206896551724138No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG10571.1534264513312964No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG10101.1021388040157136No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG9891.0792230467044959No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG9631.0508511567001309No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG9621.0497599301615015No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9060.988651243998254No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG8790.9591881274552597No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG8720.9515495416848537No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG8680.947184635530336No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG8060.879528590135312No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG7860.8577040593627236No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG7660.8358795285901354No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG7310.7976865997381056No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG7220.7878655608904408No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC7060.7704059362723702No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC7040.7682234831951112No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6760.7376691401134876No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6270.684199039720646No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6200.6765604539502401No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6070.6623745089480576No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC5980.6525534701003928No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG5720.624181580096028No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5680.6198166739415103No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5520.6023570493234396No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5450.5947184635530336No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5420.5914447839371453No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC5390.5881711043212571No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5320.5805325185508512No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5290.5772588389349629No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG5020.5477957223919686No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4980.5434308162374509No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG4810.5248799650807507No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4750.5183326058489743No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4480.48886948930597995No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4470.4877782627673505No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG4430.4834133566128328No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC4320.4714098646879092No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4170.4550414666084679No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4040.4408555216062855No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3850.4201222173723265No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3800.41466608467917937No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3710.40484504583151465No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3700.4037538192928852No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3650.3982976865997381No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3640.3972064600611087No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3630.39611523352247924No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3490.38083806198166736No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3430.37429070274989085No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG3280.3579223046704496No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG3250.3546486250545613No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3210.3502837189000436No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3020.3295504146660847No Hit
GACTACCTGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2880.3142732431252728No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2860.312090790048014No Hit
GACTACATGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2850.31099956350938457No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG2770.3022697512003492No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA2730.2979048450458315No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG2550.278262767350502No Hit
GACTACATGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2470.26953295504146657No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG2450.26735050196420773No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2430.2651680488869489No Hit
GACTACCTGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2410.26298559580969005No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG2400.2618943692710607No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG2360.257529463116543No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2290.24989087734613707No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG2280.24879965080750766No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG2230.24334351811436056No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG2230.24334351811436056No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2150.23461370580532517No Hit
GACTACTTGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2150.23461370580532517No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2030.22151898734177217No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG1870.20405936272370143No Hit
GACTACTTGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1870.20405936272370143No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1730.18878219118288955No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1710.1865997381056307No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1670.18223483195111306No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1620.17677869925796594No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1520.16586643387167177No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1520.16586643387167177No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1510.16477520733304235No Hit
GACTACCTGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG1480.16150152771715406No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG1460.15931907463989523No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG1440.1571366215626364No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1430.156045395024007No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1420.15495416848537757No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1390.15168048886948932No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG1360.14840680925360103No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1340.1462243561763422No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1320.14404190309908338No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGCGCTTAAG1300.14185945002182454No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1270.13858577040593628No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG1260.13749454386730686No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG1250.13640331732867744No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTGCTCAG1240.13531209079004802No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG1220.13312963771278916No Hit
GACTACATGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG1200.13094718463553034No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1200.13094718463553034No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG1190.12985595809690093No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1170.1276735050196421No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG1140.12439982540375383No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG1140.12439982540375383No Hit
GACTACTTGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG1140.12439982540375383No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1120.12221737232649499No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG1120.12221737232649499No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1110.12112614578786557No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1110.12112614578786557No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCACAATGGGCGCAAGCCTGAT1100.12003491924923614No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG1100.12003491924923614No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC1080.1178524661719773No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1070.11676123963334788No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTGCTCAG1060.11567001309471846No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTCCTCAG1050.11457878655608904No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTCCTCAG1050.11457878655608904No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTGCTCAG1050.11457878655608904No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1040.11348756001745963No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1030.11239633347883021No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1030.11239633347883021No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1010.11021388040157137No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTGCTCAG1010.11021388040157137No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1010.11021388040157137No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTGCTCAG1000.10912265386294195No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1000.10912265386294195No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGCGCTTAAG980.10694020078568311No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG970.10584897424705368No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG970.10584897424705368No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTCCTCAG950.10366652116979484No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTGCTCAG940.10257529463116542No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA930.101484068092536No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG930.101484068092536No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)