FastQCFastQC Report
Tue 21 Feb 2023
EGAF00007902692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007902692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60926
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC23883.91950891245117No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC8841.4509404851787415No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG8791.442733808226373No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7611.2490562321504777No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG6941.1390867609887405No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG6541.0734333453697928No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG6431.0553786560745821No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC6281.030758625217477No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC6271.0291172898270033No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG5960.9782358927223188No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG5790.9503331910842662No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG5590.9175064832747925No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG5070.8321570429701605No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4700.7714276335226341No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4510.7402422611036339No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC4190.6877195286084758No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3340.5482060204182123No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC3330.5465646850277386No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3220.528509995732528No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3190.5235859895611069No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3080.5055313002658963No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2820.4628565801135804No Hit
CCTACGGGGGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT2680.4398778846469487No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2440.4004858352755802No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2410.3955618291041591No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2340.3840724813708433No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2240.3676591274661064No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA2180.3578111151232643No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2140.35124577356136955No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2120.34796310278042214No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2110.34632176738994846No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2050.3364737550471063No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2030.33319108426615895No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1980.3249844073137905No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1960.3217017365328431No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1850.30364704723763253No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1830.3003643764566852No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1800.2954403702852641No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGACAATGGTCGGAAGACTGAT1800.2954403702852641No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1750.2872336933328956No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC1730.28395102255194826No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1710.2806683517710009No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1620.26589633325673767No Hit
GACTACATGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1600.2626136624757903No Hit
GACTACTTGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1590.26097232708531665No Hit
GACTACCTGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1590.26097232708531665No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1580.2593309916948429No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1550.25440698552342184No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1550.25440698552342184No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1500.2462003085710534No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1450.23799363161868498No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1450.23799363161868498No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1440.23635229622821127No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1420.2330696254472639No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1400.2297869546663165No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG1400.2297869546663165No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1390.22814561927584284No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1380.22650428388536914No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA1360.22322161310442176No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1320.216656271542527No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1310.21501493615205333No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1280.21009092998063222No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1270.20844959459015855No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1260.20680825919968487No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1190.19531891146636907No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1180.19367757607589534No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1160.19039490529494796No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC1140.1871122345140006No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1140.1871122345140006No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1130.1854708991235269No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1120.1838295637330532No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1100.18054689295210585No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1070.17562288678068477No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1060.17398155139021107No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1030.16905754521879002No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG1020.16741620982831631No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG1020.16741620982831631No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1000.16413353904736894No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1000.16413353904736894No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG980.16085086826642156No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGTCGGAAGACTGAA960.15756819748547418No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC940.1542855267045268No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG940.1542855267045268No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG930.15264419131405313No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC920.15100285592357943No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT910.14936152053310572No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG900.14772018514263205No Hit
CCTACGGGCGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT880.14443751436168467No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG870.14279617897121097No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT860.1411548435807373No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG850.1395135081902636No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG850.1395135081902636No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA840.1378721727997899No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG840.1378721727997899No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC830.13623083740931624No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG830.13623083740931624No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG820.13458950201884254No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG810.13294816662836884No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC810.13294816662836884No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC810.13294816662836884No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG800.13130683123789516No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG800.13130683123789516No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG790.12966549584742146No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG790.12966549584742146No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG790.12966549584742146No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG790.12966549584742146No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT780.12802416045694778No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGCCGAAAGTCTGAC780.12802416045694778No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG780.12802416045694778No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG770.12638282506647408No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT760.1247414896760004No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC760.1247414896760004No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT760.1247414896760004No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG760.1247414896760004No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG750.1231001542855267No Hit
GACTACCTGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG740.12145881889505301No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC740.12145881889505301No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA740.12145881889505301No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT740.12145881889505301No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG730.11981748350457934No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG730.11981748350457934No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG710.11653481272363195No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG700.11489347733315824No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC700.11489347733315824No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG700.11489347733315824No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCACAATGGGCGCAAGCCTGAT700.11489347733315824No Hit
CCTACGGGGGGCAGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT690.11325214194268457No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGCAAGTCTGAC690.11325214194268457No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT690.11325214194268457No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTCAG680.11161080655221088No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG670.10996947116173718No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT670.10996947116173718No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG660.1083281357712635No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA660.1083281357712635No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG660.1083281357712635No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGCCTGAC650.10668680038078981No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC650.10668680038078981No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA650.10668680038078981No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG640.10504546499031611No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA640.10504546499031611No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA630.10340412959984244No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG630.10340412959984244No Hit
CCTACGGGAGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT620.10176279420936875No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG620.10176279420936875No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG610.10012145881889505No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG610.10012145881889505No Hit
GACTACATGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG610.10012145881889505No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG610.10012145881889505No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)