FastQCFastQC Report
Tue 21 Feb 2023
EGAF00007902700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007902700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89431
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC27573.0828236293902562No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT17611.9691158546812626No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC11551.291498473683622No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC10601.1852713264975232No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG9371.0477351254039429No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG8750.9784079346088045No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG8250.9224989097740157No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC7630.8531717189788777No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG7370.8240990260647874No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG7270.8129172210978296No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG7220.8073263186143508No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG7060.7894354306672183No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7010.7838445281837395No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG6940.7760172647068689No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC6830.7637172792432154No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG6680.7469445717927788No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG6530.7301718643423422No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG6520.7290536838456463No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG6370.7122809763952097No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6250.6988628104348604No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6160.6887991859645984No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT6140.6865628249712068No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG6120.6843264639778153No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC6110.6832082834811195No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG6040.6753810200042492No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG5940.6641992150372913No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5700.6373628831165927No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC5540.6194719951694603No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5420.6060538292091109No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5410.6049356487124151No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG5290.5915174827520658No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5170.5780993167917166No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5160.5769811362950208No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA5140.5747447753016292No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5120.5725084143082376No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4740.5300175554337981No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC4690.5244266529503192No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4590.5132448479833616No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4530.5065357650031868No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4520.5054175845064911No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4490.5020630430164037No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4410.49311759904283753No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4410.49311759904283753No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4240.47410853059900926No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4030.450626740168398No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3910.4372085742080487No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3870.4327358522212656No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3820.42714494973778666No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3660.4092540617906542No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3630.40589952030056686No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3590.4014267983137838No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3570.3991904373203923No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3010.33657232950542876No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2850.31868144155829636No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2760.3086178170880344No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2720.30414509510125126No Hit
GACTACCTGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2490.2784269436772484No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2450.2739542216904653No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2420.27059968020037795No Hit
GACTACATGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2340.2616542362268117No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2340.2616542362268117No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG2180.24376334827967933No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2170.24264516778298353No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2160.24152698728628777No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2080.23258154331272152No Hit
GACTACCTGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2070.23146336281602578No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2000.22363609933915532No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1820.20350885039863137No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1810.20239066990193558No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1790.20015430890854402No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG1790.20015430890854402No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1770.19791794791515246No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1770.19791794791515246No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1760.19679976741845667No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG1740.19456340642506514No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1740.19456340642506514No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG1720.1923270454316736No Hit
GACTACTTGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1720.1923270454316736No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1720.1923270454316736No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1700.19009068443828203No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG1700.19009068443828203No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCACAATGGGCGCAAGCCTGAT1620.1811452404647158No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1620.1811452404647158No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1610.18002705996802004No Hit
GACTACATGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1610.18002705996802004No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1600.17890887947132425No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1600.17890887947132425No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG1580.1766725184779327No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1580.1766725184779327No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1560.17443615748454117No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA1540.1721997964911496No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1540.1721997964911496No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1520.16996343549775805No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG1450.1621361720208876No Hit
GACTACTTGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1430.15989981102749606No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1430.15989981102749606No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG1390.15542708904071295No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1390.15542708904071295No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG1360.15207254755062563No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG1350.15095436705392984No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1340.14983618655723407No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1320.14759982556384252No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1320.14759982556384252No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1320.14759982556384252No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGCGCTTAAG1280.1431271035770594No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1260.14089074258366785No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1250.13977256208697206No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1230.13753620109358053No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1230.13753620109358053No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1210.13529984010018897No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCAGGCTTTCGCTTCTCAG1210.13529984010018897No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1200.13418165960349318No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1180.13194529861010165No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1180.13194529861010165No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1140.12747257662331854No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1140.12747257662331854No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1140.12747257662331854No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1130.12635439612662278No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1120.12523621562992698No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGCGCTTAAG1120.12523621562992698No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1100.12299985463653544No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1090.12188167413983966No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1090.12188167413983966No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1080.12076349364314388No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1070.11964531314644809No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1070.11964531314644809No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1030.115172591159665No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1020.11405441066296922No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1010.11293623016627344No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG970.10846350817949034No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG960.10734532768279456No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG960.10734532768279456No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGCGCTTAAG960.10734532768279456No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGCCTGAC940.105108966689403No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG940.105108966689403No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA900.1006362447026199No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)