FastQCFastQC Report
Fri 24 Feb 2023
EGAF00007902728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007902728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57777
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC31885.517766585319418No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC12362.139259566955709No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG11501.9904114093843572No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC9311.6113678453363793No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG8441.4607888952351282No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG8381.4504041400557315No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG8281.433096214756737No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC8111.4036727417484467No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC8031.3898264015092512No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG7991.3829032313896534No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG7701.3327102480225694No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG7521.3015559824843794No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG6881.1907852605708154No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG6481.1215535593748378No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC6411.1094380116655418No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG4690.8117416965228379No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4120.7130865223185696No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4030.6975093895494747No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA3940.6819322567803797No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC3580.6196237257039999No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3580.6196237257039999No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3090.5348148917389273No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2990.5175069664399328No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2980.5157761739100334No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2970.5140453813801339No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2950.510583796320335No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2870.49673745608113956No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2460.42577496235526247No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2460.42577496235526247No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2380.41192862211606696No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2380.41192862211606696No Hit
GACTACATGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2230.3859667341675753No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2230.3859667341675753No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2230.3859667341675753No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2220.3842359416376759No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2170.3755819789881787No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2150.3721203939283798No Hit
GACTACCTGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2130.36865880886858093No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2110.36519722380878206No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2010.34788929850978767No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1960.3392353358602904No Hit
GACTACTTGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1960.3392353358602904No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1900.3288505806808938No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1770.30635027779220103No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1670.28904235249320664No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1610.27865759731381No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1590.27519601225401114No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1590.27519601225401114No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1580.2734652197241117No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG1570.27173442719421226No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1510.26134967201481557No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1500.25961887948491613No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1480.25615729442511725No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1460.2526957093653184No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1430.24750333177562003No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1430.24750333177562003No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC1400.24231095418592175No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1380.23884936912612287No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1380.23884936912612287No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1350.23365699153642455No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1330.23019540647662562No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1320.22846461394672618No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1270.21981065129722901No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1270.21981065129722901No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1230.21288748117763123No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG1210.20942589611783236No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1190.20596431105803348No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1170.2025027259982346No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGCCGAAAGTCTGAC1110.19211797081883794No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1100.1903871782889385No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1100.1903871782889385No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG1100.1903871782889385No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG1100.1903871782889385No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1080.18692559322913962No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1060.18346400816934075No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1050.1817332156394413No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1040.18000242310954184No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1040.18000242310954184No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1040.18000242310954184No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1010.17481004551984355No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGCGCTTAAG990.17134846046004465No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG980.1696176679301452No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTCAG970.16788687540024577No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG970.16788687540024577No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA960.16615608287034633No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA950.1644252903404469No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG930.160963705280648No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA910.15750212022084914No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGCAAGTCTGAC900.1557713276909497No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG900.1557713276909497No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG900.1557713276909497No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC890.15404053516105026No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG880.1523097426311508No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA880.1523097426311508No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG870.15057895010125136No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG850.14711736504145248No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC850.14711736504145248No Hit
GACTACATGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG840.14538657251155304No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGCGCTTAAG830.1436557799816536No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG820.14192498745175416No Hit
GACTACCTGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG820.14192498745175416No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGCAACCCTGAC820.14192498745175416No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG810.14019419492185473No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG810.14019419492185473No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG810.14019419492185473No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTCAG800.1384634023919553No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT790.13673260986205585No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG770.13327102480225694No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG760.1315402322723575No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGCGCTTAAG740.12807864721255863No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG730.1263478546826592No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGCGCTTAAG710.1228862696228603No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC710.1228862696228603No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTCAG700.12115547709296087No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG700.12115547709296087No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG700.12115547709296087No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGCGCTTCAG690.11942468456306143No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC690.11942468456306143No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG680.117693892033162No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG680.117693892033162No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG680.117693892033162No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG680.117693892033162No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG670.11596309950326253No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA660.11423230697336309No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC660.11423230697336309No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG660.11423230697336309No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC660.11423230697336309No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGCGCTTAAG660.11423230697336309No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT650.11250151444346367No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGCCGCAAGTCTGAC650.11250151444346367No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCGCACCTTCGCGCTTAAG640.11077072191356423No Hit
GACTACTTGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG640.11077072191356423No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGCGCTTAAG630.10903992938366479No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG620.10730913685376535No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG620.10730913685376535No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG610.1055783443238659No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGCGCTTAAG600.10384755179396646No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC600.10384755179396646No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG600.10384755179396646No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGCGCTTAAG600.10384755179396646No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA590.10211675926406702No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATTAG590.10211675926406702No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)