FastQCFastQC Report
Thu 2 May 2024
EGAF00007903234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007903234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508962676
Total Bases76.8 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC82425441.6194790676556408Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGGGAAACTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT21470110.4218405594833834No Hit
CCTGGGAAACTTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA15098060.2966437562506057No Hit
CCTGGGAAACTGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC6851290.13461281785621546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)