FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007905984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007905984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70375734
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA8058231.1450296205791615No Hit
CAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAG4472460.6355116665639324No Hit
CGCAAATCAGCCACTTTAATTAAGCTAAGCCCTTACTAGACCAATGGGAC4394620.6244510359209895No Hit
TGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTA4284750.6088391205980175No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3966580.563628934939421No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2631540.37392718348060144No Hit
CTGAACGCAAATCAGCCACTTTAATTAAGCTAAGCCCTTACTAGACCAAT2382370.338521513679701No Hit
CCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAGCTAAG1870990.2658572626752284No Hit
CTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATT1258640.1788457367989938No Hit
GGACTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTA1085050.1541795642230886No Hit
TGAACGCAAATCAGCCACTTTAATTAAGCTAAGCCCTTACTAGACCAATG824580.11716822733244957No Hit
CTTTAATTAAGCTAAGCCCTTACTAGACCAATGGGACTTAAACCCACAAA752000.10685501340561507No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)