FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007905991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007905991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64458277
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAGG8167721.2671328462596045No Hit
GTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTA8061431.2506431098057431No Hit
GCTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAG7205301.1178238599210462No Hit
CGGCTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCT5393140.8366869626378627No Hit
ATTCGGCTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGG4395850.6819682753853318No Hit
GCCAGACTTAGGGCTAGGATGATGATTAATAAGAGGGATGACATAACTAT4068920.6312486447628751No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3959380.6142547061876941No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATTATACTAAGTTGAGATGATATCAT3942590.6116499204594004No Hit
GTTCTTGGGTGGGTGTGGGTATTATACTAAGTTGAGATGATATCATTTAC3903190.6055374393578655No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3680130.57093210853278No Hit
CTGTTCTTGGGTGGGTGTGGGTATTATACTAAGTTGAGATGATATCATTT2921960.45331028628022435No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAATTGGTATTAGGAT2845180.441398705087944No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG2833950.4396564928348922No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA2818520.4372626963019815No Hit
GGATGACATAACTATTAGTGGCAGGTTAGTTGTTTGTAGGGCTCATGGTA2730670.42363372511492975No Hit
CTTGGGTGGGTGTGGGTATTATACTAAGTTGAGATGATATCATTTACGGG2724720.4227106473851295No Hit
TGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAG2660120.41268866060444026No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA2649180.4109914386945217No Hit
GTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAGGATGA2580210.40029149398455066No Hit
CTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTT2378690.36902785968045654No Hit
CTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTT2209290.34274729372614166No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2025100.31417222027203734No Hit
GTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCTTCGA2002660.310690898548219No Hit
GGAAAACCCGGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGG1994120.3093660105125057No Hit
CCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAGGATGATGATTAA1959060.3039268331668251No Hit
CTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGAAATGAATGAG1942510.3013592808259519No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA1803640.27981511202975534No Hit
CTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAGGATGATG1747520.27110870493792444No Hit
CGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATG1681330.26084004696557433No Hit
CTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATCGGGAT1668460.25884340656514915No Hit
TGTTCTTGGGTGGGTGTGGGTATTATACTAAGTTGAGATGATATCATTTA1618890.2511531606716698No Hit
GGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGT1607530.24939078033376533No Hit
CTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGC1566480.2430223196937144No Hit
CAGACTTAGGGCTAGGATGATGATTAATAAGAGGGATGACATAACTATTA1503960.23332302227066976No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA1500990.23286225910134087No Hit
CTTTTTTGTCTAGATTTTATGTATACGGGTTCTTCGAATGTGTGGTAGGG1457220.22607182007052407No Hit
GGACTTTTCGCTTCGAAGCGAAGGCTTCTCAAATCATGAAAATTATTAAT1443360.22392159194698924No Hit
CTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAGGATGATGATTAAT1426300.22127491865784743No Hit
GGCTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTA1425370.22113063928159296No Hit
CGGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGT1293790.2007174346282945No Hit
TGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGC1289910.20011549486499616No Hit
GGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGAT1265470.19632389491267352No Hit
GGAGGCCATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTC1265390.19631148378353333No Hit
GCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATT1220490.18934573755361161No Hit
TAGGACTTTTCGCTTCGAAGCGAAGGCTTCTCAAATCATGAAAATTATTA1217710.18891445081599062No Hit
CGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCC1195320.18544088604788492No Hit
GTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGA1193520.185161635642231No Hit
TGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGA1165860.18087048774201642No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTAT1156890.17947888988717461No Hit
CTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAA1155550.17927100347407673No Hit
TGGAGGCCATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTT1150790.1785325412902365No Hit
TAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTA1140380.17691754311087154No Hit
CTACTATTAGGACTTTTCGCTTCGAAGCGAAGGCTTCTCAAATCATGAAA1133800.17589672773909237No Hit
ATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGAT1130130.17532736718978698No Hit
CCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAATTGGTATTAGGATT1104390.17133408638893652No Hit
CGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCTTCGAATG1082530.16794274535138443No Hit
AAATGAATGAGCCTACAGATGATAGGATATTTCATGTGGTGTATGCATCG1061320.16465224473809625No Hit
CCTGTTCTTGGGTGGGTGTGGGTATTATACTAAGTTGAGATGATATCATT1032820.16023077998190985No Hit
CCAGACTTAGGGCTAGGATGATGATTAATAAGAGGGATGACATAACTATT1025400.15907964775415886No Hit
TCGGCTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGC1017830.15790524465927006No Hit
GTTTTCTCGTGTGAATGAGGGTTTTATGTTGTTAATGTGGTGGGTGAGTG1009200.15656639410327397No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA998840.15495915287962167No Hit
TTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTA998360.15488468610478062No Hit
CAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTT944170.14647769750345638No Hit
CAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAGGAT941720.1460976066735386No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC922370.14309566481275943No Hit
CGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGG922090.14305222586076882No Hit
TAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATG920230.1427636671082598No Hit
GTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTA915520.14203296188013217No Hit
CCGGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTG911040.1413379386482825No Hit
CTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACT847740.13151763271612116No Hit
CGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAG843990.13093586103767557No Hit
CTAGATTTTATGTATACGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCA837320.12990108314561372No Hit
CACTCATAGGCCAGACTTAGGGCTAGGATGATGATTAATAAGAGGGATGA833700.1293394795520209No Hit
CTTTTCGCTTCGAAGCGAAGGCTTCTCAAATCATGAAAATTATTAATATT831420.12898576237152598No Hit
GGACGATTAGTTTTAGCATTGGAGTAGGTTTAGGTTATGTACGTAGTCTA827930.12844432686278598No Hit
ATTAGTTTTAGCATTGGAGTAGGTTTAGGTTATGTACGTAGTCTAGGCCA819680.1271644291702057No Hit
GGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGG810460.12573404653680084No Hit
ATTTGAGTATTTTGTTTTCAATTAGGGAGATAATTGGTATTAGGATTAGG806050.12504988304294884No Hit
GTTTTCAATTAGGGAGATAATTGGTATTAGGATTAGGATTGTTGTGAAGT801920.12440915850108746No Hit
CTCATAGGCCAGACTTAGGGCTAGGATGATGATTAATAAGAGGGATGACA794320.12323010123277107No Hit
TCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAGGA789930.12254904052120413No Hit
ATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTT787550.12217980942928401No Hit
AGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTC785130.12180437277279378No Hit
CTCGGCTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGG776580.12047793334593786No Hit
TGAGCCTACAGATGATAGGATATTTCATGTGGTGTATGCATCGGGGTAGT763460.11844250816694961No Hit
CTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCG756670.11738911358117748No Hit
GGCAGGTTAGTTGTTTGTAGGGCTCATGGTAGGGGTAAAAGGAGGGCAAT756040.11729137593919861No Hit
GTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA753150.1168430238990099No Hit
CAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCA752440.11673287512789086No Hit
GTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAA745480.11565310689269587No Hit
TTCGGCTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGG731440.11347495372859565No Hit
CGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAG730830.11338031886890182No Hit
CCCGGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGT729140.11311813376581568No Hit
TGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATT723740.11228038254885404No Hit
CTTAGGGCTAGGATGATGATTAATAAGAGGGATGACATAACTATTAGTGG721150.11187857224294097No Hit
GATTAATAAGAGGGATGACATAACTATTAGTGGCAGGTTAGTTGTTTGTA720080.11171257339069117No Hit
GGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAG711500.11038147979040769No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG707310.10973144690169115No Hit
GGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTTTTCTCGTGTGAAT706530.10961043839257448No Hit
GGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGG670280.10398664550093388No Hit
TGAGTATTTTGTTTTCAATTAGGGAGATAATTGGTATTAGGATTAGGATT661420.1026121129486598No Hit
TTTATGTATACGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATA659810.10236233897471382No Hit
TGGGTGGGTGTGGGTATTATACTAAGTTGAGATGATATCATTTACGGGGG657490.10200241622964884No Hit
GAACAAACGAACCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCA656870.10190622997881249No Hit
CGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAA655620.10171230608599731No Hit
CTCATGGTAGGGGTAAAAGGAGGGCAATTTCTAGATCAAATAATAAGAAG650060.10084973261075532No Hit
GCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAG649180.10071321019021343No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)