FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007905992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007905992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64458277
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACCCTCCTAGCCTTACTACTAATAATCATTACATTTTGACTACCAC11491681.7828090564691947No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATCATTACATTTTGACTAC5249980.8144772470415242No Hit
CACCCTCCTAGCCTTACTACTAATAATCATTACATTTTGACTACCACAAC4686530.7270641131161479No Hit
CGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGC4041140.6269388801689502No Hit
ATTTTGACTACCACAACTCAACGGCTACATAGAAAAATCCACCCCTTACG3936640.6107268427296001No Hit
CCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTC3812140.5914120230052069No Hit
CCTAGCCTTACTACTAATAATCATTACATTTTGACTACCACAACTCAACG2974390.4614442300404648No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT2916330.4524368530669847No Hit
CACAACTCAACGGCTACATAGAAAAATCCACCCCTTACGAGTGCGGCTTC2905390.4507396311570661No Hit
TAATAATCAACACCCTCCTAGCCTTACTACTAATAATCATTACATTTTGA2716530.42144005803940426No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT2708610.42021135625452727No Hit
GAAAAATCCACCCCTTACGAGTGCGGCTTCGACCCTATATCCCCCGCCCG2377570.3688541038724941No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG2278960.35355583581608924No Hit
AAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACC2097350.3253810212767555No Hit
CTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTAC2062360.31995270366907264No Hit
CGACGTTACTCGGACTACCCCGATGCATACACCACATGAAATATCCTATC1926590.2988894661270577No Hit
TCAACACCCTCCTAGCCTTACTACTAATAATCATTACATTTTGACTACCA1858520.28832914661991355No Hit
CTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAA1835640.28477956368582424No Hit
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT1831850.2841915864428086No Hit
ATCAACACCCTCCTAGCCTTACTACTAATAATCATTACATTTTGACTACC1722520.26723022708162053No Hit
ATAATCAACACCCTCCTAGCCTTACTACTAATAATCATTACATTTTGACT1689960.2621788975215704No Hit
CTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATA1676940.26015898625400735No Hit
CAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGG1638540.25420164426672465No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1638150.2541411400121663No Hit
CAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCGAACTACT1632460.25325839845207154No Hit
CTTACTACTAATAATCATTACATTTTGACTACCACAACTCAACGGCTACA1452120.22528061058783808No Hit
CTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATG1441550.22364079015019278No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC1393750.21622514048893984No Hit
CAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCC1388590.21542462265939874No Hit
CAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCT1384810.21483819680752558No Hit
CCACAACTCAACGGCTACATAGAAAAATCCACCCCTTACGAGTGCGGCTT1279420.19848808555649106No Hit
CTCACTTCTAGGAATACTAGTATATCGCTCACACCTCATATCCTCCCTAC1265170.19627735317839784No Hit
CTCGGACTACCCCGATGCATACACCACATGAAATATCCTATCATCTGTAG1241240.19256487417434381No Hit
AATAATCAACACCCTCCTAGCCTTACTACTAATAATCATTACATTTTGAC1232990.19128497648176354No Hit
CGCACTCACAGTCGCATCATAATCCTCTCTCAAGGACTTCAAACTCTACT1229850.19079783966301178No Hit
CAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTG1215880.18863054623690917No Hit
AACACCCTCCTAGCCTTACTACTAATAATCATTACATTTTGACTACCACA1213850.18831561383497733No Hit
AATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCC1192350.184980122878556No Hit
CTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTG1186550.1840803160158935No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG1165720.18084876826602111No Hit
CGGCTACATAGAAAAATCCACCCCTTACGAGTGCGGCTTCGACCCTATAT1158660.17975348611940092No Hit
CCGAAACCAAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCT1148940.17824553392887No Hit
AAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACAATTTTACTGG1114650.1729258137011636No Hit
CCCTCCTAGCCTTACTACTAATAATCATTACATTTTGACTACCACAACTC1086160.16850590033611973No Hit
CAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCCAA1052500.16328391775039225No Hit
CGACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCAC1036110.16074118766779943No Hit
CTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGG1021450.15846684825286286No Hit
ATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTC1019870.15822172845234445No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1005690.15602185581224892No Hit
CTCCGATCCGTCCCTAACAAACTAGGAGGCGTCCTTGCCCTATTACTATC1002420.15551455090864436No Hit
ATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCT987400.15318436141257702No Hit
CTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAA986920.15310989463773597No Hit
GCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACC967800.15014363477323478No Hit
GGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTA931590.1445260474461643No Hit
TTTGACTACCACAACTCAACGGCTACATAGAAAAATCCACCCCTTACGAG878350.1362664410033796No Hit
GGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTAC869370.13487329175739524No Hit
CACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTG865230.13423101582439134No Hit
AGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAG864270.13408208227470927No Hit
TAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAAC861200.13360580519395515No Hit
ATTACATTTTGACTACCACAACTCAACGGCTACATAGAAAAATCCACCCC859820.13339171321628718No Hit
ACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAAT852710.13228867411395437No Hit
AATCATTACATTTTGACTACCACAACTCAACGGCTACATAGAAAAATCCA850130.13188841519918382No Hit
CTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAA831960.12906953749322217No Hit
CCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGA831530.12900282767409374No Hit
GCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTC828050.12846294355649623No Hit
CTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGA818990.12705738318137172No Hit
TAATCAACACCCTCCTAGCCTTACTACTAATAATCATTACATTTTGACTA815250.12647716289406866No Hit
CGAAACCAAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTA812690.12608000676158315No Hit
TATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTT812160.12599778303102951No Hit
GAAATATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATA798040.1238072187377891No Hit
GGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATA796310.12353882807013288No Hit
CGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAA775330.12028400945312268No Hit
GTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAA770420.1195222764021446No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC765590.1187729544803067No Hit
CGTTACTCGGACTACCCCGATGCATACACCACATGAAATATCCTATCATC752460.11673597791017593No Hit
CTCTGTGCTAGTAACCACGTTCTCCTGATCAAATATCACTCTCCTACTCA743770.1153878190073247No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT742290.11515821311823149No Hit
GGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTG740280.11484638349858467No Hit
CCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCG732920.11370455961768881No Hit
TAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTAT721490.1119313195417867No Hit
CGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAA720280.11174360121354159No Hit
TTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACT714340.1108220748748838No Hit
CACAATTCTCCGATCCGTCCCTAACAAACTAGGAGGCGTCCTTGCCCTAT712490.11053506751351733No Hit
GTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAA711540.11038768535497777No Hit
AACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCC708340.10989124018937087No Hit
CGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGAT697030.10813661680717901No Hit
CTCAACGGCTACATAGAAAAATCCACCCCTTACGAGTGCGGCTTCGACCC693760.10762931190357447No Hit
GTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCC687900.10672019669405684No Hit
CTCCTAGCCTTACTACTAATAATCATTACATTTTGACTACCACAACTCAA675050.10472665907591666No Hit
AATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACAG672560.1043403626814288No Hit
CACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAA671320.10414799017975612No Hit
CCCCGATGCATACACCACATGAAATATCCTATCATCTGTAGGCTCATTCA669960.1039370009843732No Hit
CTTAATTTTAATAATCAACACCCTCCTAGCCTTACTACTAATAATCATTA664720.1031240720256919No Hit
CTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTC662090.10271605615520875No Hit
ACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCC656780.1018922674585298No Hit
CTAATAATCATTACATTTTGACTACCACAACTCAACGGCTACATAGAAAA653910.10144701820062611No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)