FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007906009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007906009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62876308
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTA7657171.2178148246236087No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC2725250.4334303470871731No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2647990.4211427299452761No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT2273180.3615320416077865No Hit
CTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGG2270000.36102628672154224No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2048740.32583656152330065No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT1967730.3129525353174363No Hit
GGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGT1816640.28892281652415086No Hit
CTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTT1733730.2757366097258764No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT1569830.24966955757007872No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1549460.24642986353460827No Hit
GCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATT1396020.22202639506123675No Hit
GGAGGCCATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTC1382520.21987932243095445No Hit
GTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCTTCGA1130130.1797386067896989No Hit
GTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTA1111670.17680268377080918No Hit
GGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGAT1068940.17000680128992307No Hit
TTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTA1036650.16487132164312193No Hit
TTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAG902850.14359144624076844No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA885240.140790709276378No Hit
TGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTA876220.13935614667451532No Hit
CTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTT869940.1383573602953914No Hit
CTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGC795860.12657549803973858No Hit
TGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGA771090.12263601736921322No Hit
CGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATG739360.11758960147596452No Hit
GTCAAAAGAAAGCTCTTTATTGATTGACTCAGCAATGCAGGGCTGGCACC721840.11480317832910927No Hit
TGGAGGCCATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTT720980.11466640185043943No Hit
TTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCTTCGAA680470.10822359353542196No Hit
GTTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAG679690.10809954045011676No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA676570.10760332810889595No Hit
TCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGG630870.10033508964934774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)