FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007906079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007906079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47730969
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4397530.9213158861283541No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3577360.7494840509104267No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3129620.6556791252237096No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT2172040.45505885288019193No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC2054730.43048151819419384No Hit
GTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTA1973140.4133877944107944No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT1855800.3888041744972745No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA1588770.33285936432591595No Hit
GTTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAG1445970.30294168132224597No Hit
CTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGG1442100.3021308869719364No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG1395830.292436971057512No Hit
GTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTA1377700.2886385985585166No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT1364180.2858060560220347No Hit
TGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAG1319490.27644316209042397No Hit
ATTCGGTTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGG985350.20643829795284485No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA975260.20432436642968632No Hit
GGGGAATGCTGGAGATTGTAATGGGTATGGAGACATATCATATAAGTAAT962640.20168038071885783No Hit
AGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGG935100.1959105418538643No Hit
TGGCTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTAT909310.1905073412609746No Hit
TAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAG875230.1833673227962332No Hit
GGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTT847790.17761843468964564No Hit
GGAAAACCCGGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGG844210.17686839753871328No Hit
GGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTT816140.17098751965416836No Hit
TGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGC799750.1675536903514362No Hit
CGGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGT796320.16683507933811273No Hit
TGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTA775130.1623956136318959No Hit
GTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGA754140.1579980494424909No Hit
GGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATT720060.15085803097774947No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA670110.14039312715398677No Hit
CTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGC648430.13585100273158082No Hit
GGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTT641800.13446196744926758No Hit
GTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCC621680.13024667485799418No Hit
CTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATTGGGAT616680.12919913693769763No Hit
TAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTA605270.12680865540358083No Hit
CCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGATT602350.12619689325812766No Hit
GGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGA598840.12546152163807944No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC588580.12331197382563089No Hit
TTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGATTAGGATTGTTGTGA572610.11996613770820366No Hit
GTTTTCAATTAGGGAGATAGTTGGTATTAGGATTAGGATTGTTGTGAAGT548340.11488138864308411No Hit
GTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAA526410.11028688732466335No Hit
CGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT523660.10971074146850024No Hit
GTTTGGTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCCTAATGTGGG518850.10870300998917495No Hit
GGTAGGAGTAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGGGGTGGG517610.10844322058494141No Hit
CAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTT505840.10597731632056327No Hit
GGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAAT499840.1047202708162074No Hit
CTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCG498240.10438505868171248No Hit
CGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGG494460.1035931200139683No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT494280.10355540864883761No Hit
CCGGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTG491420.10295621695842797No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTAT481470.10087161649703781No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)