FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007906081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007906081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36077818
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTA4043071.1206525849207398No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2695040.7470074825478636No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT2681890.7433625836240983No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC2616160.7251436325777796No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2072070.5743335142940186No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2037320.5647015570620152No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT1967780.5454265554529933No Hit
CTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGG1729660.4794247811771765No Hit
GTTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAG1574180.4363290485028779No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT1441420.39953081419724434No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA1250060.34648991244426147No Hit
ATTCGGTTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGG1137600.31531840423387025No Hit
TGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTA1108290.30719429872394166No Hit
CTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTT972860.2696559974885399No Hit
CTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTT966990.26802895895755113No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA943420.26149585875731174No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG903980.2505639337722697No Hit
TGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAG840690.23302129857188147No Hit
CTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGC833690.2310810481942117No Hit
GGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGT821050.22757751036939097No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA754300.20907583712518313No Hit
GGGGAATGCTGGAGATTGTAATGGGTATGGAGACATATCATATAAGTAAT749450.2077315207920834No Hit
GGAGGCCATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTC747450.2071771635413206No Hit
CGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATG741730.20559170180413905No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT708750.19645035073906075No Hit
GGAAAACCCGGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGG701200.19435765211743128No Hit
GTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCTTCGA686840.19037736705695452No Hit
TGGAGGCCATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTT649580.18004969147524386No Hit
GGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGAT633070.17547347237019711No Hit
CTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATTGGGAT603630.16731333363896897No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC585890.16239618482470308No Hit
GCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATT549630.15234568786837385No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA548650.1520740528155001No Hit
TGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGA540360.14977624201108838No Hit
CCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGATT533250.1478055019846267No Hit
TGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGC520350.14422989771720673No Hit
GTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTA517860.14353972294000708No Hit
CTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGAAATGAATGAG495080.137225593853819No Hit
CTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAA492920.13662688802299516No Hit
CGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCC490680.13600600790214087No Hit
GTTTTCAATTAGGGAGATAGTTGGTATTAGGATTAGGATTGTTGTGAAGT486720.13490838054563056No Hit
GTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGA474050.13139652736204832No Hit
GTTTTCTCGTGTGAATGAGGGTTTTATGTTGTTAATGTGGTGGGTGAGTG469900.13024623606671556No Hit
GTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTA467410.12955606128951588No Hit
CGGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGT466830.1293952976867947No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA457360.12677041610443293No Hit
GGTAGGAGTAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGGGGTGGG450310.12481630679549412No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTAT448400.12428689562101566No Hit
ATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGAT444900.1233167704321808No Hit
GGACTTTTCGCTTCGAAGCGAAGGCTTCTCAAATCATGAAAATTATTAAT429360.11900941459375398No Hit
TGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGG427230.11841902412169161No Hit
ATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTT422190.11702204384976941No Hit
GCCAGACTTAGGGCTAGGATGATGATTAATAAGAGGGATGACATAACTAT420120.11644828409522992No Hit
CTTTTTTGTCTAGATTTTATGTATACGGGTTCTTCGAATGTGTGGTAGGG410830.11387329466543682No Hit
AGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGG405840.11249017332478366No Hit
TTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTA405020.11226288685197093No Hit
TGGCTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTAT404150.11202174144788911No Hit
CGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT381530.10575196094176206No Hit
ATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGATTAGG381090.10563000234659423No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG378030.10478183575292718No Hit
CGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCTTCGAATG377420.10461275679144452No Hit
CTTGGATTAAGGCGACAGCGATTTCTAGGATAGTCAGTAGAATTAGAATT368910.10225396668944892No Hit
AGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTC367290.10180493731633104No Hit
CGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAA363750.10082372498248092No Hit
CGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGG363110.10064633066223683No Hit
CTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCG361980.10033311881555587No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)