FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007906082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007906082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36077818
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT1711440.4743745866227275No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1681590.466100804655093No Hit
CAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCAC1272810.35279572617168814No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC1102750.3056587291393288No Hit
CGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGC1084370.30056418600481877No Hit
CCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTC1063290.29472126058177905No Hit
CTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTAC946230.2622747306946335No Hit
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT943640.2615568380548957No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG918140.2544887831076702No Hit
CAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGTG869460.24099572762410407No Hit
CTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAA824570.22855317913073347No Hit
CGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATC814160.22566774964051317No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT759790.21059754777852696No Hit
GGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTG749280.20768440042576852No Hit
CTCGATGTTGGATCAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTTC743160.20598806723843444No Hit
CTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATA737720.20448021551635964No Hit
GAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATA734440.2035710696251087No Hit
CCTCGATGTTGGATCAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTT730600.20250670370364418No Hit
GGACATCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATT709660.19670258328815782No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT695550.19279159288402642No Hit
CTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATG649600.1800552350477515No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT646190.17911005593520096No Hit
CAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCGAACTACT613480.17004354309897565No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC603810.16736322579153762No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC596820.16542574720012168No Hit
CATGATTTGAGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAGAAG548040.15190497385401744No Hit
AAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACC544760.15099582796276648No Hit
CAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGG535860.1485289381968721No Hit
CGCACTCACAGTCGCATCATAATCCTCTCTCAAGGACTTCAAACTCTACT526040.14580704409562686No Hit
CGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAG522090.14471218852537035No Hit
CACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAAC518280.14365613796266727No Hit
CTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCT514100.14249753130857304No Hit
CTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAG508190.14085940563256902No Hit
GGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTA505170.14002232618391722No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT498640.13821234976017674No Hit
CTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGG498540.1381846318976386No Hit
GGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTAC478540.13264105939001078No Hit
GGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATA453880.1258058344881057No Hit
ATTTTGACTACCACAACTCAACGGCTACATAGAAAAATCCACCCCTTACG437120.12116032072671357No Hit
CCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCA436920.1211048850016373No Hit
CACAACTCAACGGCTACATAGAAAAATCCACCCCTTACGAGTGCGGCTTC420530.1165619273316363No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT403570.1118609778451679No Hit
AACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCC402470.11155608135724837No Hit
GAAAAATCCACCCCTTACGAGTGCGGCTTCGACCCTATATCCCCCGCCCG400070.11089085265633306No Hit
CAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCC397490.11017573180284905No Hit
CTCCGATCCGTCCCTAACAAACTAGGAGGCGTCCTTGCCCTATTACTATC392130.1086900543708048No Hit
CTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGA390070.10811906640251913No Hit
GTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAA380670.10551358732393407No Hit
CTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAG379430.10516988582846114No Hit
CCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGC379260.1051227654621463No Hit
CTTAATTCCATCCACCCTCCTCTCCCTAGGAGGCCTGCCCCCGCTAACCG373200.10344306299233508No Hit
TTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTC363020.10062138458595252No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAAC362740.10054377457084573No Hit
CGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGAT362720.1005382309983381No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)