FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007906101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007906101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64555680
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAG9221211.428411876383302No Hit
ATTCGGTTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGG7060841.0937596815648134No Hit
GTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTA5415500.838888227960731No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT5247480.8128610836412845No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC4905660.7599114438884386No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT3875050.600264763689268No Hit
CTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGG3413430.5287575005018923No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT3309300.512627238997405No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA3090650.47875725265383307No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2858330.44276971445425095No Hit
GCCAGACTTAGGGCTAGGATGATGATTAATAAGAGGGATGACATAACTAT2245690.3478686925767028No Hit
TGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTA2151060.33321002892386853No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2082010.32251383611790624No Hit
GTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAGGATGA2057570.31872795701323264No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA1858650.28791424704998847No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG1738970.26937521221990074No Hit
CTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATCGGGAT1676570.2597091379100956No Hit
GGATGACATAACTATTAGTGGCAGGTTAGTTGTTTGTAGGGCTCATGGTA1615700.2502800683069251No Hit
CTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGC1585350.24557870043348623No Hit
GTTCGGTTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGG1552260.24045289275862325No Hit
TGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAG1536360.23798990267006717No Hit
CCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAGGATGATGATTAA1485410.23009749103409644No Hit
CTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAGGATGATGATTAAT1445240.22387495569715943No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA1423450.22049957494057842No Hit
CTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTT1401110.21703899641363858No Hit
CTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAGGATGATG1367130.21177532325583123No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT1363570.21122386132405388No Hit
TGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGC1269230.1966101201319543No Hit
GGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGT1231360.1907438663801543No Hit
CTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTT1227620.19016452154171407No Hit
CCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGATT1176960.18231703236647806No Hit
GGAAAACCCGGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGG1042430.1614776577367011No Hit
CTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAA1040410.16116474956192856No Hit
GGAGGCCATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTC1038340.1608440961353052No Hit
CTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGAAATGAATGAG1035870.16046148069387542No Hit
CGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATG1034380.16023067218872142No Hit
ATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGAT997120.15445891050950125No Hit
GTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCTTCGA980190.15183636823281854No Hit
CGGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGT960750.14882501431322542No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA941200.14579662083956052No Hit
GTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTA928630.14384946452426806No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA924100.14314774470658506No Hit
GTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGA907200.14052984958101286No Hit
GTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA904950.14018131324772662No Hit
TCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAGGA902070.1397351867411202No Hit
GTTTTCAATTAGGGAGATAGTTGGTATTAGGATTAGGATTGTTGTGAAGT863910.13382401052858556No Hit
TGGAGGCCATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTT848510.1314384729585375No Hit
CGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAA833230.12907152399293137No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTAT831390.12878649872482173No Hit
GGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGAT827900.12824588014563554No Hit
CAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAGGAT815520.1263281557873761No Hit
ATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGATTAGG812330.12583400871929473No Hit
CGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAG807400.1250703268868053No Hit
TGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGG807120.1250269534764408No Hit
CTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCG805540.12478220351795535No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG788680.12217050459386376No Hit
CTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACT777890.12049907924445998No Hit
CAGACTTAGGGCTAGGATGATGATTAATAAGAGGGATGACATAACTATTA762310.11808565876774901No Hit
GGACTTTTCGCTTCGAAGCGAAGGCTTCTCAAATCATGAAAATTATTAAT749190.11605330468209768No Hit
GCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATT748810.11599444076803157No Hit
TGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGA733640.11364453135649721No Hit
TGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATT712270.110334210715463No Hit
GCTTTATTGGCTTATAGACCTGGGCAGTCCAGGTGTAGATCTGGTTCCAG712170.1103187202117614No Hit
GGGGAATGCTGGAGATTGTAATGGGTATGGAGACATATCATATAAGTAAT710030.10998722343254692No Hit
GTCAAAAGAAAGCTCTTTATTGATTGACTCAGCAATGCAGGGCTGGCACC705670.10931183747115668No Hit
CGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCC702730.10885641666232933No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA699420.10834368098980601No Hit
CCGGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTG694950.10765125547434401No Hit
TAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATG681100.10550582071167092No Hit
TGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGATTAGGATT678330.1050767337591363No Hit
AGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTC672540.10417983359481305No Hit
TTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTA668930.1036206264111849No Hit
TAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTA668290.10352148718749457No Hit
TCGGTTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGC666520.10324730527197608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)