FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007906102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007906102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64555680
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCAC7287811.1289184778163595No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC3391250.5253217067808751No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT2957310.45810221501810533No Hit
CACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAAC2952780.4574004952004223No Hit
ATTTTGACTACCACAACTCAACGGCTACATAGAAAAATCCACCCCTTACG2565670.39743520632111695No Hit
CGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGC2375990.3680528188999016No Hit
CACAACTCAACGGCTACATAGAAAAATCCACCCCTTACGAGTGCGGCTTC2308910.3576617890168611No Hit
GAAAAATCCACCCCTTACGAGTGCGGCTTCGACCCTATATCCCCCGCCCG2202880.3412372079420432No Hit
CTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAA2028110.31416445462273807No Hit
CCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTC1954840.3028145625605679No Hit
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT1940650.3006164600853093No Hit
CCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAACG1885650.2920966830494234No Hit
CAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTG1763810.27322305333938085No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT1616650.25042722809209045No Hit
CAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGG1598010.2475397982021102No Hit
TAATAATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGA1570660.24330314543971965No Hit
CAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCGAACTACT1451940.22491281944516736No Hit
CTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTAC1347940.20880269559549214No Hit
TCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCA1324530.20517636867894506No Hit
CGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATC1231860.19082131889866236No Hit
CTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTC1204140.18652735127257586No Hit
CCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTT1186920.18385988653515847No Hit
ATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACC1114730.17267729191296569No Hit
CTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATA1089590.16878297928238073No Hit
GAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATA1069540.16567713329020778No Hit
AAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACC1067890.1654215399791312No Hit
CGGCTACATAGAAAAATCCACCCCTTACGAGTGCGGCTTCGACCCTATAT1036690.16058850282422862No Hit
GGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTA1018950.15784048746756288No Hit
ATAATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACT1002960.1553635559256753No Hit
CAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCCAA951360.1473704560156442No Hit
CCACAACTCAACGGCTACATAGAAAAATCCACCCCTTACGAGTGCGGCTT947440.14676322827054103No Hit
GGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTAC938050.14530866997295977No Hit
CTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATG933920.14466891217008324No Hit
CGACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCAC907360.14055463438693544No Hit
CTTACTACTAATAATTATTACATTTTGACTACCACAACTCAACGGCTACA902970.1398746012744347No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT894740.13859973281979215No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTAC877830.13598028864384978No Hit
CTCCGATCCGTCCCTAACAAACTAGGAGGCGTCCTTGCCCTATTACTATC846030.13105430846673755No Hit
GGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATA808320.12521283952086013No Hit
CGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGAT796090.12331835091815313No Hit
CTCACTTCTAGGAATACTAGTATATCGCTCACACCTCATATCCTCCCTAC791670.12263367065454193No Hit
AACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACA782320.12118530855844134No Hit
CGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAA775480.12012575810525115No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG765730.1186154339943441No Hit
GTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAA757690.1173699974967346No Hit
CTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAG751160.11635846760501942No Hit
AATAATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGAC747460.11578531896805982No Hit
CTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGC742570.11502783333705105No Hit
TAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAAC742350.11499375422890752No Hit
AACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCC739400.11453678436971No Hit
GGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTG712800.11041631038508154No Hit
CAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCC710470.11005538164883401No Hit
CGCACTCACAGTCGCATCATAATCCTCTCTCAAGGACTTCAAACTCTACT704370.10911046092303574No Hit
CTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGA697530.10805091046984558No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGAC696780.1079347316920835No Hit
CCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTC696470.10788671113060849No Hit
AATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACAG691330.10709049924034571No Hit
GTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAA687440.10648791864635303No Hit
GTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGA679690.10528740460947822No Hit
ATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTC677120.10488929866434682No Hit
CTCCACCTTACTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAG674840.10453611517995008No Hit
CTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGG673830.10437966109256383No Hit
CAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCT652880.10113440056707636No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)