FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007906115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007906115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40612736
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTA6911831.7018873094390885No Hit
GTTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAG4043790.9956950450223299No Hit
ATTCGGTTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGG2884860.7103338223753258No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2604230.6412348087063132No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2362050.5816032684919332No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2089880.5145873452111179No Hit
CTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTT1819610.44803925546902335No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT1750410.4310002655324674No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC1663100.4095020832873707No Hit
GGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGT1457810.3589539005695159No Hit
GTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCTTCGA1397650.3441408133645564No Hit
GGAGGCCATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTC1338520.32958134118321897No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT1289470.3175038490388828No Hit
GCCAGACTTAGGGCTAGGATGATGATTAATAAGAGGGATGACATAACTAT1256870.3094768104271527No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT1242350.3059015772786153No Hit
CTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTT1189360.29285394611187976No Hit
CTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGG1102890.27156259553653317No Hit
TGGAGGCCATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTT1095080.26963955346421375No Hit
GGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGAT1028230.2531791997466017No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA986480.24289917330366514No Hit
GCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATT974160.2398656421473303No Hit
GGATGACATAACTATTAGTGGCAGGTTAGTTGTTTGTAGGGCTCATGGTA941420.2318041315906419No Hit
TGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGA925970.22799990623630972No Hit
GTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAGGATGA914380.22514612165011488No Hit
CGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATG895370.2204653239811275No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG872010.21471343373664853No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA855990.21076885832070016No Hit
TGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAG772730.1902679001976129No Hit
ATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTT734940.18096293734064112No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA724570.17840955113194049No Hit
CTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGAAATGAATGAG720980.1775255919719371No Hit
TGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTA717190.1765923871762789No Hit
CCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAGGATGATGATTAA707290.174154728211367No Hit
CGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCTTCGAATG704250.17340619454941425No Hit
CTTTTTTGTCTAGATTTTATGTATACGGGTTCTTCGAATGTGTGGTAGGG682790.16812213784365573No Hit
CTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAGGATGATGATTAAT677570.16683682675306583No Hit
CTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAGGATGATG658700.16219050102903682No Hit
CGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCC652360.1606294143787801No Hit
CTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGC643250.1583862756747046No Hit
GGACTTTTCGCTTCGAAGCGAAGGCTTCTCAAATCATGAAAATTATTAAT641910.15805632991581753No Hit
GTTCGGTTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGG621480.15302588823368118No Hit
CTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATCGGGAT574600.1414827112361994No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA572720.1410198022610444No Hit
CATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTT558060.13741009716754862No Hit
TGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTG557100.13717371811640564No Hit
CCATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTT538520.13259879856407608No Hit
TTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTA515840.12701434348082336No Hit
TAGGACTTTTCGCTTCGAAGCGAAGGCTTCTCAAATCATGAAAATTATTA481720.11861303803811692No Hit
CAGACTTAGGGCTAGGATGATGATTAATAAGAGGGATGACATAACTATTA467810.11518800407832655No Hit
CCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGATT467620.11514122072445451No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT458020.11277743021302479No Hit
CTACTATTAGGACTTTTCGCTTCGAAGCGAAGGCTTCTCAAATCATGAAA454640.11194517897045893No Hit
CTAGATTTTATGTATACGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCA453920.1117678946821017No Hit
CGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT433840.1068236328623612No Hit
CTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAA425010.10464943804820241No Hit
ATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGAT412440.10155434984729912No Hit
GTTTTCAATTAGGGAGATAGTTGGTATTAGGATTAGGATTGTTGTGAAGT409590.10085259953921843No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)