FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007906116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007906116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40612736
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACCCTCCTAGCCTTACTACTAATAATTATTACATTCTGACTACCAC3702620.9116893774406137No Hit
CCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTC1882750.46358610264523914No Hit
CGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGC1747430.4302665055612111No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTCTGACTAC1704190.4196195991326464No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1613110.397193136655457No Hit
CACCCTCCTAGCCTTACTACTAATAATTATTACATTCTGACTACCACAAC1540500.3793145086309871No Hit
CGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATC1495220.3681652967187436No Hit
GAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATA1363790.3358035272482012No Hit
CTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTAC1350840.3326148723395538No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT1191660.2934202709219098No Hit
CTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATA1140490.2808207750396329No Hit
GGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTG1067330.26280672151711226No Hit
CACAACTCAACGGCTACATAGAAAAATCCACCCCTTACGAGTGCGGCTTC1062950.26172824209627243No Hit
CTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATG1036760.2552795261072783No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1036620.25524505416231996No Hit
CCTAGCCTTACTACTAATAATTATTACATTCTGACTACCACAACTCAACG983110.242069384342882No Hit
CATGATTTGAGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAGAAG980630.24145873846076268No Hit
CTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAA851920.2097667096351253No Hit
CTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAG828540.20400989482708085No Hit
CCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCA814900.20065134247542446No Hit
GAAAAATCCACCCCTTACGAGTGCGGCTTCGACCCTATATCCCCCGCCCG782560.19268832319004559No Hit
TAATAATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTCTGA757450.18650553363358727No Hit
CTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGG739400.18206111501574285No Hit
CTGACTACCACAACTCAACGGCTACATAGAAAAATCCACCCCTTACGAGT733980.18072655828949816No Hit
CAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCT712840.1755212946007873No Hit
CCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGA698490.17198792024255644No Hit
CTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCT697940.17185249474450576No Hit
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT666540.1641209299467044No Hit
CAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCC661670.16292179871851037No Hit
CGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAA661490.16287747764642105No Hit
AAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACC602100.14825398613873245No Hit
TCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTCTGACTACCA600370.14782801139031854No Hit
CGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATC579880.14278279601748572No Hit
CCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTT576870.14204164920088122No Hit
CTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTC564440.1389810329449363No Hit
ATTCTGACTACCACAACTCAACGGCTACATAGAAAAATCCACCCCTTACG563260.13869048369457307No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT543110.13372898590235338No Hit
ATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTCTGACTACC542680.13362310778569558No Hit
TTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTC535530.131862576311037No Hit
AAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATAT534730.13166559376841788No Hit
CCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATT506950.12482537497596814No Hit
CAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGG490920.12087833727823705No Hit
CTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAG488820.1203612581038618No Hit
CAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCGAACTACT486540.11979985785739725No Hit
CAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTG477760.11763797445215214No Hit
ATAATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTCTGACT475130.11699039434329173No Hit
CGGCTACATAGAAAAATCCACCCCTTACGAGTGCGGCTTCGACCCTATAT472920.11644623006930634No Hit
CCACAACTCAACGGCTACATAGAAAAATCCACCCCTTACGAGTGCGGCTT460140.11329943395096552No Hit
ATTTGAGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAGAAGAACC440850.10854969239206146No Hit
ATTACATTCTGACTACCACAACTCAACGGCTACATAGAAAAATCCACCCC425970.1048858170993454No Hit
CAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCCAA420870.10363005339014836No Hit
GTCAAAACTAGTTAATTGGAAGTTAACGGTACTATTTATACTAAAAGAGT420360.10350447701922864No Hit
CTTACTACTAATAATTATTACATTCTGACTACCACAACTCAACGGCTACA411470.10131550851437343No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)