FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007906195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007906195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64311954
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTA9715471.5106787145668128No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC4204150.6537120610578867No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT4071540.6330922552905173No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT3518930.5471657726338093No Hit
CTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGG3132970.4871520464142638No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT2983870.4639681761185487No Hit
GGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGT2786200.433232055116845No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2483260.38612728202909213No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2431450.37807123695852873No Hit
GGAGGCCATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTC2208780.3434478137610311No Hit
CTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTT2048120.3184664549299808No Hit
CTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTT1746280.27153272313884286No Hit
TGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTA1725280.268267389294376No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA1686440.26222807660299047No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA1683540.26177714954827835No Hit
GTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCTTCGA1679730.26118472469363935No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG1674120.2603124140809032No Hit
GCTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAG1553040.2414854320862339No Hit
GCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATT1550750.24112935520509918No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA1545320.24028503316817273No Hit
TTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTA1527940.23758258068165677No Hit
TGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAG1505530.2340980029933471No Hit
GGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGAT1422700.22121859335824257No Hit
CGGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGT1370380.21308324732288494No Hit
CGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATG1345110.20915396226337643No Hit
TGGAGGCCATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTT1316690.20473487712719787No Hit
CTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGC1272620.19788234081645223No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA1239900.1927946397025971No Hit
TGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGA1225770.19059753650153438No Hit
GTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGA1189540.1849640581593898No Hit
CTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAGG1185350.18431254631137473No Hit
CTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATTGGGAT1179700.18343401601512527No Hit
TGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGC1168830.18174381702039405No Hit
CCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGATT1087810.169145848064265No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT1072080.16669995752267144No Hit
GCTTTATTGGCTTATAGACCTGGGCAGTCCAGGTGTAGATCTGGTTCCAG1061320.16502686265760172No Hit
ATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTT1032530.1605502454489254No Hit
GGAAAACCCGGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGG1028820.1599733698030696No Hit
CGGCTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCT1002420.15586837868431117No Hit
TTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAG971200.15101391570220365No Hit
CTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGAAATGAATGAG969040.15067805279248708No Hit
CGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCC908010.14118837067211487No Hit
GTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTA907070.141042208109553No Hit
CTTTTTTGTCTAGATTTTATGTATACGGGTTCTTCGAATGTGTGGTAGGG892590.13879068267774913No Hit
CCGGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTG859870.133702981563894No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA858790.1335350501090357No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTAT857810.13338266786296057No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG833650.1296259790209453No Hit
ATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGATTAGG832880.12950625011331487No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA814190.1266001029917393No Hit
GTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTA813840.12654568076099817No Hit
GGACTTTTCGCTTCGAAGCGAAGGCTTCTCAAATCATGAAAATTATTAAT812550.12634509596769522No Hit
TTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGATTAGGATTGTTGTGA811490.12618027435459356No Hit
ATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGAT789880.12282009033654925No Hit
CGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCTTCGAATG786920.12235983375656724No Hit
GTTTTCAATTAGGGAGATAGTTGGTATTAGGATTAGGATTGTTGTGAAGT780300.121330476135121No Hit
TTTATGTATACGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATA778630.12107080434844196No Hit
CTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAA773120.12021404294448898No Hit
TTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCTTCGAA768770.11953765236242084No Hit
TGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTG740520.11514500088117366No Hit
CGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGG735100.11430223376512554No Hit
GGGGAATGCTGGAGATTGTAATGGGTATGGAGACATGTCATATAAGTAAT728630.11329619995685405No Hit
ATTCGGCTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGG725130.1127519776494429No Hit
TGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGG714570.11110998120193952No Hit
GCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGT695890.10820538900124228No Hit
TAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTA694450.10798148039476456No Hit
TCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGG692820.10772802829159879No Hit
CGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAA669780.10414549058795508No Hit
CATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTT669410.10408795851545732No Hit
CCATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTT668730.10398222389573174No Hit
GTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA665700.1035110828696015No Hit
TGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGATTAGGATT663360.10314723138407518No Hit
TAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATG661950.10292798754023241No Hit
GCCATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTT657400.1022204985405979No Hit
CTACTATTAGGACTTTTCGCTTCGAAGCGAAGGCTTCTCAAATCATGAAA650330.10112116947962738No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC645400.10035459348661682No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)