FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007906196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007906196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64311954
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGC2708090.42108656813630635No Hit
CTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTAC2672900.4156148015655068No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT2453320.3814718489194093No Hit
CCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTC2420930.37643546019453866No Hit
GAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATA2287970.3557612321964281No Hit
CTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATA2038980.31704525724719856No Hit
CGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATC1862860.2896599907382693No Hit
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT1637700.25464939224207056No Hit
CTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATG1570000.24412257789586056No Hit
CAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCAC1532970.23836470588345054No Hit
CCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGA1501710.2335040232178298No Hit
CAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGTG1411780.21952061975911977No Hit
CTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAA1383680.21515129209104733No Hit
CTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGG1355310.21073998155926038No Hit
CTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAG1355100.21070732822081567No Hit
CTCCGATCCGTCCCTAACAAACTAGGAGGCGTCCTTGCCCTATTACTATC1269680.19742519407822687No Hit
GGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTG1201690.18685328702654566No Hit
CAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGG1116940.17367533258280413No Hit
CAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCGAACTACT1080980.16808383710437408No Hit
CCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATT1077080.16747741796183024No Hit
CCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCA1036020.16109291283545824No Hit
CTCGATGTTGGATCAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTTC983400.1529109191737511No Hit
TTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACT974410.1515130453041436No Hit
CAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCT951880.14800980856529408No Hit
CCTCGATGTTGGATCAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTT919050.14290500332177747No Hit
TCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCAT888130.1380971879660195No Hit
GGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTA882780.13726530529611958No Hit
GGACATCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATT882190.13717356496429886No Hit
AAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACC870110.1352952205432912No Hit
CACAATTCTCCGATCCGTCCCTAACAAACTAGGAGGCGTCCTTGCCCTAT867970.13496246747533125No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC865860.13463437917000626No Hit
TTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTC802640.12480416937728249No Hit
CCGAAACCAAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCT801710.12465956173559896No Hit
CCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGC796170.12379813556901101No Hit
CAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCC781410.12150307235261426No Hit
CGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAA775990.12066030523656614No Hit
GGCGTCCTTGCCCTATTACTATCCATCCTCATCCTAGCAATAATCCCCAT770970.1198797349556507No Hit
GGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTAC767110.11927953549662011No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT750650.11672013573090936No Hit
GAAAAATCCACCCCTTACGAGTGCGGCTTCGACCCTATATCCCCCGCCCG745280.11588514321925283No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT729230.11338949520955312No Hit
CTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCT723850.11255294777701824No Hit
GTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAA723500.11249852554627714No Hit
CGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATC721590.11220153565851848No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG712970.11086119386140873No Hit
CGCACTCACAGTCGCATCATAATCCTCTCTCAAGGACTTCAAACTCTACT710190.11042892585723643No Hit
AAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATAT708690.1101956877254888No Hit
GGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATA707070.10994379054320134No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC694250.1079503819771982No Hit
CATGATTTGAGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAGAAG684010.10635814299780101No Hit
CAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCCAA679130.10559934160918201No Hit
CGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAG676660.10521527615223758No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG672900.10463062590199015No Hit
CGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGAT661760.10289844404354437No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660310.10267298051618833No Hit
TCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATAC652250.10141971428826435No Hit
CGACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCAC646330.10049920112830035No Hit
TTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACT646000.10044788873931586No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)