FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007906209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007906209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65522561
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTA11451601.7477338836007952No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC5059010.7721019939986777No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT4909740.749320527932356No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA4526120.6907727553567389No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT4201610.6412463029337331No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG4082510.6230693577438159No Hit
CTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGG3824220.5836493478940787No Hit
TGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAG3622440.5528538483103552No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT3573500.5453846652910895No Hit
CTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTT3399440.518819769575246No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA3251330.49621534176602167No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3207860.48958098570048264No Hit
GGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGT3146490.48021474618490567No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2866950.43755157860816823No Hit
TTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTA2806000.4282494391511956No Hit
CTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTT2779850.42425844740714574No Hit
CGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATG2744060.41879620669894146No Hit
GGAGGCCATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTC2657430.40557480651588085No Hit
GTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCTTCGA2570180.3922587824367854No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA2349920.3586428802744752No Hit
GGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTTTTCTCGTGTGAAT2209570.3372227773575578No Hit
CTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATCGGGAT2077660.3170907803802113No Hit
TGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGC2076630.3169335826174438No Hit
TGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTA2069810.3158927197610606No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA1976280.3016182471866446No Hit
GCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATT1875080.28617318544676545No Hit
CTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGC1852420.28271483466587943No Hit
CGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCC1831500.27952204127063957No Hit
TAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTA1716660.2619952538179941No Hit
GGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGAT1704560.2601485616534433No Hit
GCTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAG1559300.23797909852760488No Hit
TGGAGGCCATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTT1512220.23079378719644367No Hit
ATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGAT1408400.21494886318622375No Hit
GGAAAACCCGATAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGG1392380.2125039038080334No Hit
TGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGA1375300.2098971680914609No Hit
CAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTT1338220.2042380486318293No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA1315730.20080564311275925No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC1311220.20011733057869943No Hit
TTTATGTATACGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATA1304030.19901999862306968No Hit
CTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAA1303580.19895131998885085No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT1299610.19834542181585363No Hit
CTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGAAATGAATGAG1278640.19514499746125613No Hit
CTTTTTTGTCTAGATTTTATGTATACGGGTTCTTCGAATGTGTGGTAGGG1253470.19130357252061622No Hit
CCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGATT1247730.19042753838635823No Hit
GCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAG1247660.19041685504325756No Hit
GGACTTTTCGCTTCGAAGCGAAGGCTTCTCAAATCATGAAAATTATTAAT1247090.19032986210658034No Hit
ATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTT1227570.18735073557335466No Hit
CAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCA1216820.18571007931146036No Hit
GTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA1212600.1850660263416749No Hit
GTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAA1208290.18440823764504566No Hit
CGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCTTCGAATG1204510.18383133711760746No Hit
CGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAA1195550.18246386920071697No Hit
GTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTA1193830.18220136419881391No Hit
CTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAGG1184130.18072095808343022No Hit
GGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAG1142450.174359790362895No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1136770.17349291337986622No Hit
GGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGG1117960.1706221464695191No Hit
TTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCTTCGAA1100570.1679680988049292No Hit
ATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGC1092840.16678835248823684No Hit
CGGCTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCT1083810.16541020122824565No Hit
GGGGGATAGGTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTT1069340.16320180159014236No Hit
CGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAG1045780.15960609354081873No Hit
CTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCG1022280.15601954264272425No Hit
CTAGATTTTATGTATACGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCA1018050.15537396348106722No Hit
TTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAG1007480.15376077867286048No Hit
GTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGG994120.15172178633249697No Hit
CTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACT981160.14974384166699467No Hit
ATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGATTAGG974200.14868161212441008No Hit
GTTTTCAATTAGGGAGATAGTTGGTATTAGGATTAGGATTGTTGTGAAGT973820.14862361683329198No Hit
GGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGG969850.14801771866029473No Hit
GTTTTCTCGTGTGAATGAGGGTTTTATGTTGTTAATGTGGTGGGTGAGTG961020.1466700912377341No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG933850.1425234279227883No Hit
GGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCA929200.14181374870252705No Hit
CGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGG911190.1390650771419023No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC902640.13776018309174454No Hit
GTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTA897840.13702761099341035No Hit
CCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGT896600.13683836320134068No Hit
TTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGATTAGGATTGTTGTGA885230.13510308304341156No Hit
GGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGG885190.13509697827592543No Hit
AATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAG885000.13506798063036637No Hit
CGATAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGT874270.13343037675221517No Hit
GAACAAACGAACCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCA872110.13310071930796477No Hit
CTACTATTAGGACTTTTCGCTTCGAAGCGAAGGCTTCTCAAATCATGAAA870560.1328641595678777No Hit
TGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGG858340.13099915310086857No Hit
TGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTG857260.13083432437874337No Hit
TGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATT847730.12937986352517572No Hit
GGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTAT833560.12721724964321832No Hit
CCATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTT828810.12649230850424176No Hit
TGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGATTAGGATT828190.12639768460820694No Hit
AGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCC815090.1243983732565032No Hit
CATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTT812260.12396646095686034No Hit
CGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGG801190.12227696655507711No Hit
CAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATAT801170.12227391417133406No Hit
GTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGA800360.12215029262974016No Hit
TAGGACTTTTCGCTTCGAAGCGAAGGCTTCTCAAATCATGAAAATTATTA797360.1216924350682813No Hit
CGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCA793400.12108806308715558No Hit
GGGGAATGCTGGAGATTGTAATGGGTATGGAGACATGTCATATAAGTAAT792480.12094765343497486No Hit
AGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTC791770.12083929381209627No Hit
GCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGT771660.11777012195845031No Hit
GATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTA768210.1172435857627726No Hit
TCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGG765030.1167582567476262No Hit
TAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTT764830.11672773291019561No Hit
GGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT758470.1157570748799028No Hit
GCCATGGGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTT757200.11556324851221857No Hit
ATTCGGCTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGG756920.11552051513981573No Hit
GAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGC740580.11302671762173641No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC738500.11270926971245827No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG737060.11248949808295802No Hit
GGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAGCCTGTGGCTACA718470.10965230739378456No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT715010.10912424500623534No Hit
CTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGG713120.10883579474251626No Hit
GGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCT705450.10766520557705307No Hit
GCCAGACTTAGGGCTAGGATGATGATTAATAAGAGGGATGACATAACTAT702290.10718292894564972No Hit
GGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAG696490.10629773766016258No Hit
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT682420.10415038569692048No Hit
CCCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAA680740.10389398546250353No Hit
GCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTT670040.10226096015996688No Hit
ATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCA664700.1014459737005701No Hit
GGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTC659240.10061267293871497No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)