FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007906210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007906210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65522561
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTAC3788150.5781443738134716No Hit
CTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATA3664060.5592058588796613No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT3370000.514326660705463No Hit
CCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTC3218440.49119569670056085No Hit
CGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATC3034610.4631397115262329No Hit
CGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGC3023780.46148684572936643No Hit
CTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATG2951460.45044942611446465No Hit
AAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACC2881790.43981644734551817No Hit
GAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATA2729840.4166259618576264No Hit
CTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAA2408870.367639781357142No Hit
CCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGA2402330.36664165187316167No Hit
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT2057440.31400482041597855No Hit
CTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGG2047460.31248168092819206No Hit
CCGAAACCAAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCT1953580.2981537916382725No Hit
CTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAG1875670.2862632307671857No Hit
CAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGG1871910.2856893826234906No Hit
CCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTT1714350.26164270349567076No Hit
CTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTC1697300.25904054635471285No Hit
CAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCGAACTACT1681510.2566306893895677No Hit
TCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCAT1651840.25210247810673947No Hit
AATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCC1646000.2512111820537662No Hit
GGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTG1640150.2503183598089214No Hit
CTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTT1518730.23178733810480945No Hit
CAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTG1516550.23145462827681598No Hit
TTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACT1515470.23128979955469078No Hit
CAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCAC1480870.2260091756791985No Hit
CCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCA1410730.21530446589229013No Hit
ATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCT1386150.21155308627207048No Hit
CCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATT1355670.20690125344764837No Hit
CGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAA1342190.2048439468048265No Hit
AAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACAATTTTACTGG1332980.20343832409114776No Hit
CAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCCAA1325390.20227994446065686No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT1279950.19534492859642652No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC1276740.1948550210056655No Hit
CGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATC1178760.17990139304841885No Hit
CAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCT1172070.1788803706863656No Hit
GGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTA1128060.17216360025976396No Hit
CTCCGATCCGTCCCTAACAAGCTAGGAGGCGTCCTTGCCCTATTACTATC1086680.16584821829537463No Hit
CGACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCAC1082940.16527742253542255No Hit
CATGATTTGAGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAGAAG1063500.1623105055371691No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG1061370.1619854266685333No Hit
TAGCAAGCCTCGCTAACCTCGCCTTACCCCCCACTATTAACCTACTGGGA1055750.1611277068367337No Hit
CAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCC1051190.1604317633433162No Hit
GTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGA1017390.1552732348175463No Hit
GTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAA1006860.15366615477682566No Hit
TAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAAC989210.15097242612357598No Hit
TTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTC980460.1496370082359876No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT975670.14890596232952494No Hit
GGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTAC973490.1485732525015315No Hit
CTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGC969560.1479734590960204No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA969540.14797040671227732No Hit
CGAAACCAAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTA962710.1469280176640226No Hit
AAACAACAAAGCATAATATTTCGCCCACTAAGCCAATCACTTTATTGACT959830.1464884744050221No Hit
CTTCAAACTCTGCTCCCACTAATAGCTTTTTGATGACTTCTAGCAAGCCT942760.14388326488032113No Hit
TCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATAC941650.14371385758258137No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGAC925380.14123074340760278No Hit
CGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAA923630.14096365983008508No Hit
ACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCC912580.1392772178120449No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG905080.13813257390839775No Hit
CTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCT902920.13780291646414736No Hit
GAAAAATCCACCCCTTACGAGTGCGGCTTCGACCCTATATCCCCCGCCCG902120.137680821114425No Hit
GGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATA874880.13352347445637847No Hit
GGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACAC873720.13334643619928105No Hit
AATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACAG870720.1328885786378222No Hit
AAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATAT867670.13242309011700565No Hit
CGCACTCACAGTCGCATCATAATCCTCTCTCAAGGACTTCAAACTCTGCT841830.12847941032097326No Hit
CCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTA830200.12670444917438436No Hit
CTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAA819770.12511263105237905No Hit
CGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGAT800840.12222354983957358No Hit
GTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAA796610.12157797067791658No Hit
CGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAG788970.12041196008806798No Hit
TTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGC778200.11876825144243065No Hit
AAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAA766780.11702534032514388No Hit
AACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCC760710.11609894185912543No Hit
CCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAA757870.11566550336761104No Hit
TTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACT752050.11477725969838083No Hit
TGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTC750440.11453154280706458No Hit
CAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAA737610.11257343863589214No Hit
CGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGC731680.11166840685607513No Hit
TAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGT729780.1113784304004845No Hit
CGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGT718570.10966756931249987No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT717910.1095668406489789No Hit
TTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTT716770.10939285477562453No Hit
GGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACC705540.10767894130389684No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC704100.10745916967439659No Hit
CACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCA703900.107428645836966No Hit
CAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACA699290.10672507138419086No Hit
AAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAA696060.10623211140968682No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC692750.10572694190021051No Hit
CAATAATCCCCATCCTCCATATATCCAAACAACAAAGCATAATATTTCGC690020.10531029151928295No Hit
CACAATTCTCCGATCCGTCCCTAACAAGCTAGGAGGCGTCCTTGCCCTAT686660.10479749105044903No Hit
CAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCC682890.10422211671488238No Hit
TATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAA682150.10410917851638919No Hit
CTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGA677210.10335523973185358No Hit
TTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCA673010.10271423914581115No Hit
ACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAAT671190.10243647222519278No Hit
AATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAA670750.10236931978284548No Hit
CTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAG669050.10210986716468547No Hit
CCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCG665120.10151007375917434No Hit
GCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGC662760.10114989247749337No Hit
GTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGT659590.10066608965421849No Hit
CTTCTAGCAAGCCTCGCTAACCTCGCCTTACCCCCCACTATTAACCTACT658460.10049362997273566No Hit
ATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT655300.1000113533413323No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)