FastQCFastQC Report
Tue 23 May 2023
EGAF00007912299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007912299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69105845
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAATCATCTTCAGACAGCCCACTCGCAATATCAGGAGGAGAATCTTCCT1364780.19749125417683555No Hit
AGAGTAGCCATTGGGGTATTCAGGGGAGGAGAAGTTGCCTGTGCTGTCTT1292970.18709994791323367No Hit
AGGTGGATGTCCCCTCAGTGGGCACCCAGCTCTGGTGTCCCACAGAGAGC1243540.1799471520824324No Hit
CCCATTCTTCGGGGAGGGTTTCCTGGTGGCCTAATAAAATAAAAAGTTAG1221000.17668548876003182No Hit
CTTTCACAGTTACTCAGGCCGAAGAATTCCTGGAAGGTGTTCAGGAGAAT1175660.17012453866963062No Hit
GGTTTGAAGTCGAGAGTCAACACTGGTGAGGGTGATGATGCCCAGGTTCC1165610.16867024779163034No Hit
TGTGACCCTGGCTATCAGCTCCAAGGACAAGCCAAAATCACCTGTGTGCA1127830.16320327173482937No Hit
GGGATGTAGGGATGAAACTCCTCAGCTGCATCCTCGAAGGCTTTGTAATC1124240.1626837787744293No Hit
GAATCAGAAGCACCTTTTCTGAGTACCGGAACACTCACCCTTTTCACAGA1101770.1594322448412287No Hit
TGCATCCATCTCAGCCCATCTTCATTCCTCCTGAGATGCCCAAAAGGGGC1101600.15940764489602868No Hit
ACCGGGATCTGATGGGTAAGGGTTTCATCTTTAATCTGCAGGAAGGGTGG1082500.1566437687000282No Hit
GGAGCAGAGACACTCAGCACAGCCGGGGTGCAGGGCAGAGGCCGCCCTCC1040020.15049667651122708No Hit
GGATGTACAGCCCTCCCCTATCAACCCTTTCAGTGCTTTCTTTGAGGAGC1030390.14910316196842685No Hit
AATGACTGTCTCTATTTTCCCGTGTCTTTCAGTAATGTATCCTGCATATG1029660.1489975269096268No Hit
ACTGTCTACGGCCTGGACAAGCAGAGAGTATCCAGACACCTGCCAAGGTA1019030.14745930680682653No Hit
GGCTTTGTGGTCACTGCTCTTCCAGCCCGGGTGGCTGTCCTTTAGTTCCC1017630.14725671902282653No Hit
TGCAGATTGAGATGGCCATCCTGCGGTTCCCTTATGAGTCCTGGGGGACC1016610.1471091193516265No Hit
GCTGCAGAGATACGACCCCTTCCCTGCCGGAGACCCCGAGCCCCGCGCAG1011390.14635375632842634No Hit
TGGCTTAGGGCAGGGTTCAGGAGGCTGGGCACTCACAGTTTCTCTTTGGT1010830.14627272121482632No Hit
GCAATCAACCCCGAGAGGTTCCGGGAGGAGTTTGAGGGGCCCCCACTGCC997860.14439589010162598No Hit
CCTTGTAATGATCAGCATGGCAATGGTTATTCAATTAGGGCTAGTGTTTC993450.14375773858202587No Hit
GGGAGATGCCACAAAGCAAGCCCAAGTTACGGAGCAGGCACACGCCTCAC993320.14373892685922587No Hit
TGCAGATTGAGATGGCCATCCTGCGGTTCCCTTACGAGTCCTGGGGGACC983300.1422889771480256No Hit
GCAGCCCTTCTCATGCACTCCCTCCTCCCCTGCCAGCCCCTCGAATTTGC972860.14077825110162534No Hit
GGACAGCATGATCTCGTGCCAGGCCTCCCCCGTGGCGCTCCTGAGAAGAA968850.14019798180602525No Hit
AGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGACA967850.1400532762460252No Hit
AAAATGAAGCGTTTGGGGTTCCTAATACCCTTTCCTGTTTCCTCATACAG962120.13922411338722504No Hit
AGCAGTGTGTATATGACCTGTGTGTGGTCGGTGGGGAGCGGCTCAGCCTG957940.13861924414642496No Hit
TGGTGTTAAACCCCTCCAAGAACAGCCCCTGGCCAAGGACGTAGCCCCTG954310.13809396296362486No Hit
CAGTCTGTCTATGGTCTCCTGGAGCAGGGAGTGGGATGGAGACAATCAAA932190.13489307597642428No Hit
GGAGCTTTCTGTTAATAGCTCTTCATCCTCCTCTTGCTCTGTTCTACGGT929140.1344517240184242No Hit
AAGAGCAAACATGCCTGCATAGTTACTCACATATAGAGGAGTACCAGGCT928150.13430846551402417No Hit
GGATGGCTGGTATGAGCTGAGGCTGCTGTGACCATGCAGTTTGAGCAATT924570.13379041960922408No Hit
CCAGGACTCTTGGGTTTGCTTGGCGATGTAACTAAAGATTCTTGGCTTTC923230.13359651415882404No Hit
CTGCTTTCCTTCTAGACCCTGAGAAATACTTTAGGAAAGAACCAGAGGAA921900.133404055764024No Hit
GCACAACTGCCCCTCACAAGAATTCAGCTTCAGTGCAAAATTCATCTGTT919350.13303505658602394No Hit
CGCAAGGAGCCATTGTTTGGAATCAGTACAGGAAACTTAATAACAGGATT912590.13205684700042375No Hit
GTCATTCAGAAGAACTCTTTTGCCATCTTAAAACTATATCAGAGAAAGAA911030.13183110632682374No Hit
AGAAAACATGACAGATGGCTCTGTGCCCTTTCCCATGAAAGACGACGAGA910560.1317630947136237No Hit
GCGGTAGAAGGTCCTAAGGTCAGAAAGACAAGAGGGAATCATCCAAAAGA907980.13138975436882364No Hit
ATCTAGGGATGGCAGTGACTGGATGTATTCAAAATACTGGTCTAAGGTTT907490.13131884864442364No Hit
CATCTGCTGCTGGTTCACCATCCTTTACCTCCCAAGTCAGAGCACCACAG900940.13037102722642346No Hit
GCGCCTGGATGCAGCTCTTGGCAGGTAGGGGAGGGGGTGGACGGAACTCC900940.13037102722642346No Hit
TTGCTGGTCAGCCACATCGCACTGGATGAACAGAGTCTTCTGAGGTTCAA896410.12971551103962337No Hit
AGCAGGAGTTCCCACCAGTTTCTTTCTGAGAACATCTGTTCAGGTTTCTC892640.12916997107842326No Hit
GGACCAAAGAGAAACTGTGAGTGCCCAGCCTCCTGAACCCTGCCCTAAGC889570.12872572500922316No Hit
CAGATCATCCACCTGCATTGGAGAAGCACGGGACTTATCAGGAGTTGTGA888300.12854194894802315No Hit
CCATGAGGAGGGTTTCGATGACACGCTGAAAGCTCGGGCAGATCTCTGCA887620.12844354916722311No Hit
GGAAGGTGCATCAGGCCAGACCACGGGAGGCCCCACACTCCAGCACCCGG885400.12812230282402307No Hit
TGAAGCTGGATTATCCTGCTCTGAAAAGAGGGGCATAGATCCTCCCATTA871930.12617311893082273No Hit
GGATGTCTGGGCAAGGCTCTTGCCACTCTCCATCTGCTGTTTCAGTCCAG864710.12512834478762253No Hit
GTACGGCAGATGTCCCATGTGCGGGTGACCCGAGCACTGCGCTGCATTTT861660.12468699282962245No Hit
GGAACAATTAAGGCTTTACCACATTGCTTACATTCATAGGGTTTCTCTCC854850.12370154796602227No Hit
GGAAACTCTGGGTAAGTGCTTGGAGGGCCAGGTTGCTGAGGGTGGGGAGG851710.12324717250762218No Hit
GCGGTGAAAGCGAGGAGACAGGCTGAATTTGAACACTTTCAGAGGAGGTA847810.12268282082362209No Hit
GCTGGCACTGTAACTCCACGTGTCCCCCAGCCATAGCCAGAATAGGGGCA847600.12265243265602208No Hit
GCAGAACTGAGATAGACCCCAAGGCCAGTGCCATAGAACAAGCTGACCAC846970.12256126815322207No Hit
TTCTGCTCCACAGAGTTGGGTCCTCAGGTCATGCCCAACAGGCCAGTGAC842940.12197810474642194No Hit
TGTGGCTGTATCATCCCACACATTCATTTTTCTTCGTCCTGCTTTGGTGC841620.12178709340722191No Hit
CAGCTACAATAGACATCCCAGGACTGGGCAGAGCACCCCGTCCCTTTTCA841170.12172197590522192No Hit
CCTGCAGATACAACATGCCAAGCACACAGGAAAAACATAAAGATTTTAAA841080.12170895240482191No Hit
TGATTTTCTCTGGGGCACTCAGAGGAAACAGCAGCCTTGCCCTTGGGGCA839930.12154254101082189No Hit
ACAGAGGGCTAAGACAGGAGAGGAAATACAAGATTTGCCTCCATGAAGGC835770.12094056588122176No Hit
TCGAAAAGAGCAGCTTAAAGAGGTACTTATGTCATAAAACTTTCCAAGCA832650.12048908453402168No Hit
CCAGTCTGTGAAAAGGGTGAGTGTTCCGGTACTCAGAAAAGGTGCTTCTG830800.12022137924802163No Hit
GGGAGGGCAGCAGAACAACGCACAGCAGGTGCCATCATCAGCAACGTGTG826800.11964255700802154No Hit
TCTGTTGGTTGGCGTATGGTTCTTTAGGATGATTGACAGTGGCAGTACCT824120.11925474610722146No Hit
CGTTGCTGATGATGGCACCTGCTGTGCGTTGTTCTGCTGCCCTCCCTGAG823860.11921712266162146No Hit
CAAGACAGCACAGGCAACTTCTCCTCCCCTGAATACCCCAATGGCTACTC823750.11920120505002146No Hit
CAGTGGTCAGCTTGTTCTATGGCACTGGCCTTGGGGTCTATCTCAGTTCT818680.11846754786082132No Hit
TGAACGACCTGTTGGAGAGAGGAATAGTCAGCATCAGCCCCATTCTGGAC818590.11845452436042131No Hit
GGGGACCTAGAAACCAGGCTCTCTGTGGGACACCAGAGCTGGGTGCCCAC818210.11839953624762131No Hit
ATGTGCTCTGATTGCCCACGACGTACACCAAGGTGACTGCCTGGGAGGCA816310.11812459568362127No Hit
TCCAGAACTGGTCATGCAGGTAGGCGTGGTGGCTACATTCATCACCAAAT814700.11789161973202121No Hit
GCGTCCCGACCACCAATCCGGTCGTAGTGGATCTCACTGCAAGGACCACA812380.11755590283282116No Hit
CCAGTCATCATCGTTTAAATGTGTAACCAAAAAGAGCCATTAAAAAAACA812010.11750236177562115No Hit
GCTGAATCCCCTACTTCGGCATATACAACAGCACCTACAGTTTCTTCTGA812010.11750236177562115No Hit
CTTTGAACGATGTCTTCCCATACTCCGCACCCTTCAGTCTCTGGTAACTA808450.11698720998202107No Hit
GTGAAACAATGTAGTCCCATCATAAGGGCAGATCTGAACATTGTTAGAAG808090.11693511598042106No Hit
TCTGACCTAGCTGCTGTCCTTCCCGCACTGGACCCTCCTCCCCCGCGCAG807590.11686276320042105No Hit
AGACAATGAGAATGACCCAAAGGCTATTTTTCTCAAAGGAGTGATGCGTG805770.11659939908122098No Hit
GGAGCATGTTGTGGACACTTGCCGGAAGTTTATTAAGGCCAGGTGAGTTG804160.11636642312962094No Hit
AAACCGCATCAACTATTGGCATCTGGAGGCACCATCTCAACGAAGACTGC803910.11633024673962093No Hit
CAAAGAAATAGTTGCCTAGATTTCACCTGTTAATTTTTACAGTGCTTCTT803050.11620579995802091No Hit
CTTCTACCAGGTGGGATCAGCAATATGTAATCCAACTCACCTCCATGTTC800930.11589902417082087No Hit
TTTGCCCTGATCTCATTGAGTAGCTGGGACAGTTCCATTCTTTTCCCATC798780.11558790721682081No Hit
CATGTCTGACACCAATTTGGCTGAGGCTGGCCTCAACTACCATGCCCAGA796600.11527244909602076No Hit
CCTTCCCATACACCTCTCTGGTTTTCTGCCAGGACCCAAAATGGCAGGGG796110.11520154337162074No Hit
CACACCCTGGTCTTATTTGGCCTATCTCCCTCCCCTTTTCTCCTGTGTTG792190.11463429757642063No Hit
GGTCCGCTGAGTTGTTGGGGGCTGCGCGGGGCTCGGGGTCTCCGGCAGGG790900.1144476274040206No Hit
CCTTCATCCTGCGAGCATTGTCCGTCTTCATCAAGGACGCAGCCCTGAAG789920.11430581595522059No Hit
CACTGGCTTCTGATTGGTGCAGGGTGTTCAACCAGAGAAGAAAGAGCCCT789220.11420452206322056No Hit
GCATGTGTATGGGCTGTGGAAGAGCCTGAGGGAGGATACGCTGTAGCTTG788010.11402942833562053No Hit
GCCTTCTACATTACCGGTGAGTGAGGGGCCACTTAGCCCTGCCTCCCACA784220.11348099426322043No Hit
GGCATGGAGTACTTGGAGAAGTCGGGCAGAGTGTGGGAGAAGGAGACGGT783690.1134043003164204No Hit
TTCAGAAGAAACTGTAGGTGCTGTTGTATATGCCGAAGTAGGGGATTCAG783220.11333628870322039No Hit
GGTAGCAACTCACCTGGCCTTAATAAACTTCCGGCAAGTGTCCACAACAT782710.11326248886762039No Hit
CCTGATGGCCTTGCTGCAAACCTTGGGACCTTCAACTGTGGCCCTGAGAA782000.11315974792002037No Hit
GTCTACGGAGATCTCCAGGAGTGAATTTTTGCGGTCTCATGTTCATCATT780990.11301359530442034No Hit
CTCAGGGCCACAGTTGAAGGTCCCAAGGTTTGCAGCAAGGCCATCAGGTC780580.11295426602482034No Hit
GTGGAGACAACCTTGTACCCTCTGCCCAGGTGGGGGCAGCTGGAGGCCCT778390.11263736084842027No Hit
CGAGTGTTACCCCTGTCTGTGACCCACTTCTATAGTGCCACCAAGTATGC775970.1122871733932202No Hit
CCATGAACCAGTATGTTAATCCAAGTTGGTAATGATATTCTGCAACTTCG775940.1122828322264202No Hit
GGATCTGTTCAGTTCTTACCCAACAGTTTTGTGTGTCTCCATAAGGATGG775620.11223652644722021No Hit
GGAGACAATTCAAGTTGTGTCTATCAAACAGTAGATACTAACTTTTTATT772500.11178504510002013No Hit
CCTTATGACTTCACACATGGCCATCTGTAGTTATCGCTTATGGTGGTCCC770640.11151589275842007No Hit
CTCTCTGGCTAAAGTAGGTGCTCAAATGCTTCTCTCTGGGGCTCTCCAAA769960.11141749297762006No Hit
AATCGGAAATTTAGATTTCCCCTGATACTGATGAGAATACCAGCTGAGAT769650.11137263425402004No Hit
GCCAGCATGTGACATACCGTCGTAGGCCTCTAGTGAGAAACTCTAACTAA769150.11130028147402003No Hit
AGAAGATTTTCGGTAAGCTTAGTGGTAGATAGAATTTCATATGATTATTC768240.11116859941442002No Hit
AGTTGCATAAATACCGAAGCAGTTCACCGATATACTGAATGACAGTGACG765570.11078223556921996No Hit
CCCCCGACTCCTTACCTCCAGCTCTGTCTCAGCGCCGTGCCCCTCGCTTC765010.11070120045561993No Hit
CTGAACCGCACTAAACCATTGTTGTTCGTTGTGCCATTGAAAAAACGCAC764960.11069396517761994No Hit
GGGATCTTGCAAAAGTCCTTGCTGCTCCTCTCGGGGGGCAGTGGGGGCCC764770.11066647112121993No Hit
TGAGATTCCCAGTTCTTCCATGATAGCGTTGAAAGAGAGACACTAGGGAA764350.11060569478601992No Hit
GGAAAGGACATTTCCAGTATTTTGTTAGGACATTTTCTGTTTGATTCTCA764310.1105999065636199No Hit
TCGGGTTCCCTGCAAGAGAGGGGCGTGGTCACTCTCCCGCTCGCCACCTC762420.11032641305521987No Hit
TCCCGGATGAAGAGGTTGGGGGGAGCCACAGGCATGAATTCGATAGAGAT761460.11018749571761983No Hit
GGAAGCAGGCCTATGGCTGTAGGAAGGAACAACACAATTGTGACAAAATT760440.1100398960464198No Hit
TGTTTTCTTGTGCAGGACCAAAAGATCTCAGATTGCTATAACTGAAGGTA758150.10970852031401974No Hit
CCGAGAGGGAGAAACCGAGTCGGGGGAGGCAGGGTCAGCGCCAGCAGGTG757570.10962459108921974No Hit
GCAGAAAATACAACGTCACTGTCATTCAGTATATCGGTGAACTGCTTCGG756050.10940463863801969No Hit
CACTGGACTGAAACAGCAGATGGAGAGTGGCAAGAGCCTTGCCCAGACAT755380.10930768591281967No Hit
GCATTTGGAGAATGAACAATGCCCCCACCAAAGGTTATGAAGAAGATGTC751930.10880845173081959No Hit
GATTTCAGGATTGCCTTCCAGGAGCTTCTGTGCCTGCGCAGGTCTTCTTT751860.10879832234161957No Hit
TCCTGGCTTGGCCAAGTACTTATTTCCTCGGTGGTTTGGTTTGGGCTGGA751040.10867966378241957No Hit
GGACCTGCCGCTCTTTCCTTCCATTATGATGCAACCTGTGCAACACCCTG750380.10858415811281955No Hit
GGTCTGTGGCATCCCTTCCAGGGGCTACGTCCTTGGCCAGGGGCTGTTCT750280.10856968755681953No Hit
TTTGCAGAGGCTTGCAGATGAACAGCGGCAGCTGCTGGGCAGTGCCCATG750120.10854653466721954No Hit
TCTCCAGCCTTCCTAGCTTCCCAAAATACAAAGGGAGAAGAATCCTATTG749600.10847128777601953No Hit
AATCCAGCCCCGAGCGAAAGAATTCCCCGCAGAGCCACCCCAGGACCTCC749330.10843221727481953No Hit
CCGACCATCGCGAACTTTCTTCTGCTTTTTGATGTTTCCATCTGCCTTCC749120.10840182910721952No Hit
ACCTGGGAACTAAAGGACAGCCACCCGGGCTGGAAGAGCAGTGACCACAA748430.1083019822708195No Hit
TGTATCTCATGTTCCCTAGTGTCTCTCTTTCAACGCTATCATGGAAGAAC746870.10807624159721944No Hit
CGGTTGTGTTGCGTGTAGCTTCCACTTTCATAAGGTAATGCCGGGGGCCT746520.10802559465121944No Hit
CGTGTGCCTGCTCCGTAACTTGGGCTTGCTTTGTGGCATCTCCCTTGTTG738980.10693451472881925No Hit
CTGTAGCTCCAGTTCCTCTGTGTGTGCACAGAAGGAAGGAAGCGCTACAC736030.10650763332681917No Hit
GCTTCTATGAGTTGGTCACCATTGGATATAATTAATAAGCAGAAAATTTG734610.10630215143161914No Hit
GGGGCTCGGTCTTTTCCAGGGCTCTTTCTTCTCTGGTTGAACACCCTGCA733670.1061661282052191No Hit
GGTTGGACAAGATGGTGGCTGTATCTGTGGTCCCACCGTCTTCGTCGTGC733620.10615889292721911No Hit
CCTGAGACTTCTCACTGTGCTATGAATTTTATCAAAGTCTGTTGATGGGT732240.10595919925441906No Hit
TGAGGACATGTGCACATGCTCAGCCAGGATCTCCAGCAGCTTCACTCCTG730460.10570162335761903No Hit
GTTTGTAACTCTACGGCGGCATAAAAATGAACCATTAATTATCAAAGATG728510.10541944751561898No Hit
GGGTACTGTCACTCACCCAGAGTTTTAGGAGTCACTGTCCAAGACAAGGT727300.10524435378801895No Hit
CCTGCACACTCGATGTCCACCCGTGGCCGGTCCACCTGGGCCAGGTTCAC725350.1049621779460189No Hit
CTCAGGACATAGGGAGGGCTTTTGTTCCATCTCAGAACCCAAGGGCCTTC725020.10491442511121889No Hit
CAGACCATGACCGTGAGCAACCCTCAGGGCTGCCGACTCTTCTATGGGGA719920.10417642675521877No Hit
CCACGCTGACCTGTTGCTGCCGCTGCAGGCGCCGCAGGCCCGCCCTGGTG718880.10402593297281872No Hit
TGGCCATCAAAGTAGAGGAGGATGAGAAGAATGAGGCCAAGAAGAAGAAA717020.10375678063121868No Hit
CCCTGTCTTGGCCTTGCAGATCCAAGGAGAAAGTCAGGGGCCAGTGCGCG716470.10367719257321865No Hit
AGAGTCACCTTCGTCCACTGTCCCGAGGCCCGCCCCCAAAGGGGGCAGGT715250.10350065179001863No Hit
TCATGGCACAGACAGGGCATGCAGAGCGGACCCATGGCATGCTCGGCCCG713930.10330964045081859No Hit
GCCCTGGCTGTTACCCTGTGCCCCTTGAGTACACCCGCTCCTTTCTCTTC713750.1032835934500186No Hit
GCAGACTTGGAACTTTTGGAAGAGCTACATTGGGAAGACTGTTGGCGATA709890.1027250299884185No Hit
GGAACAATTAAGGCTTTACCACATTGCTTGCATTTATAGGGTTTCTCTCC707250.10234300731001841No Hit
CCTCATGCCCCTATTCTGGCTATGGCTGGGGGACACGTGGAGTTACAGTG705360.10206951380161836No Hit
GCAGTTCGCACCATTTTCTCGGGGGGTCCGACGCCGAGGAGCAGGGGGGC704590.10195809052041835No Hit
TCCACCCCTGCAAGCGAACCAAAAACAAATCCTGGCACCTTGCCATTCCC704000.10187271424001833No Hit
GCTTTTCAGTTCTAATGTACCAGCCACGGAAATTGCCTGCAGGTTGGGGA702380.1016382912328183No Hit
CCACAGCTTCTAATTATAACAAAAGTGAAAGCCAAGAATCTTTAGTTACA700430.10135611539081825No Hit
CGGCATACTTGGTGGCACTATAGAAGTGGGTCACAGACAGGGGTAACACT699500.10122153922001821No Hit
GGAGGCCCCAATACAATTGGCTCTGCTTGCAAATACTGTCCCATGGACAA694850.1005486583660181No Hit
AGGAGAGAGTGATGTTTACGTTTGCTATAGTTTAATTTCTAGCAAGTAAG692060.10014492985361803No Hit
GTTCGCCTTCTGGGAGCAAAAGGAAAGTTGGATTTTAAAATCTTTATGTT691810.10010875346361803No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)