FastQCFastQC Report
Tue 23 May 2023
EGAF00007912899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007912899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50540724
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAG28730945.684710808654027No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAG15052252.978241862938093No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAG13869282.7441791296855977No Hit
CTGGCCTCGAGATCTGCCTACCTCGGCCTCCCAAAGTGCTGGGATTACAG7928521.568738904492148No Hit
CTGGCCTCGAGATCTGCCTACCTCAGCCTCCCAAAGTGCTGGGATTACAG5583221.1046972734304321No Hit
CTGGCCTCGAGATCTGCCTACCTTGGCCTCCCAAAGTGCTGGGATTACAG4249350.8407774293063155No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTATAG1481170.29306465811609667No Hit
CTGGCCTCGAGATCTGCCTGTCTCGGCCTCCCAAAGTGCTGGGATTACAG1266520.25059395666749845No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGTTGGGATTACAG1210920.239592927081931No Hit
CTGGCCTCGAGATCTGCCTGCCTCTGCCTCCCAAAGTGCTGGGATTACAG1111150.21985241050365645No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTAGGATTACAG1065360.21079238991511084No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTATAG985480.19498731359685312No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGAGATTACAG968380.19160390341855807No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAGAGTGCTGGGATTACAG853710.16891526919954689No Hit
GTGATGATGATGTCTTCAGAACAGGACAGCATCTTCAATGGGACGGGATC837340.1656762969996235No Hit
AGAATCATCTTCAGACAGCCCACTCGCAATATCAGGAGGAGAATCTTCCT833250.16486704860025353No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAA830750.16437239798939168No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCTTCCCAAAGTGCTGGGATTACAG797740.15784103132357186No Hit
CTGGCCTCGAGATCTGCCTGCCTTAGCCTCCCAAAGTGCTGGGATTACAG790300.156368951105647No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAGAGTGCTGGGATTACAG776040.15354746402129102No Hit
TGTGATCCCGCCCCATTGAAGATGCTGTCCTGTTCTGAAGACATCATCAT773720.15308842825441124No Hit
TGTGATCCCGTCCCATTGAAGATGCTGTCCTGTTCTGAAGACATCATCAT758210.15001961586462434No Hit
CTGGCCTCGAGATCTGCCTGTCTTGGCCTCCCAAAGTGCTGGGATTACAG744130.1472337436242504No Hit
CTGGCCTCGAGATCTGCCTGCCTCGACCTCCCAAAGTGCTGGGATTACAG744120.14723176502180696No Hit
CTGGCCTCGAGATCTGCCTGCTTCGGCCTCCCAAAGTGCTGGGATTACAG713940.14126034284748276No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGTCTCCCAAAGTGCTGGGATTACAG708380.140160239888926No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGAGATTACAG703420.13917885307697608No Hit
CTTTCACAGTTACTCAGGCCGAAGAATTCCTGGAAGGTGTTCAGGAGAAT679590.13446384345424098No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCTCAAAGTGCTGGGATTACAG670390.13264352920626937No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGTTGGGATTACAG665430.13166214239431948No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTTCCAAAGTGCTGGGATTACAG634920.1256254263393615No Hit
AGTGGCCCTGGAATACATCTCCAGTGGAAACCGCAGCTTGTCTGCAGTGG631860.1250199739916666No Hit
ACTGTCTACGGCCTGGACAAGCAGAGAGTATCCAGACACCTGCCAAGGTA631030.12485574998886047No Hit
AATGACTGTCTCTATTTTCCCGTGTCTTTCAGTAATGTATCCTGCATATG630940.12483794256686945No Hit
CTGGCCTCGAGATCTGCCTGCCTAGGCCTCCCAAAGTGCTGGGATTACAG629100.12447387971727512No Hit
CTGGCCTCGAGATCTGCCTGCTTTGGCCTCCCAAAGTGCTGGGATTACAG626900.12403858717971669No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGTTGGGATTACAG626590.12397725050396984No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCGAAGTGCTGGGATTACAG617880.12225388777572715No Hit
GAGAAGCCTCTAGAAGGGATTCTCTCCTCCTCTGCTTGGCACTTCACACA609270.12055031107191896No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAATGCTGGGATTACAG606360.11997453776087577No Hit
AGGTACATCCAGGCCACACATGCACACAGATGCGTGTCATGCACACCTAC603250.11935919240096363No Hit
CTGGCCTCGAGATCTGCCTGCTTCAGCCTCCCAAAGTGCTGGGATTACAG592010.11713524325452876No Hit
TTGCTGGTCAGCCACATCGCACTGGATGAACAGAGTCTTCTGAGGTTCAA590330.1168028380440296No Hit
ATCTAGGGATGGCAGTGACTGGATGTATTCAAAATACTGGTCTAAGGTTT587410.11622508613054296No Hit
ACCTCCCCTAAGTTCTTAAGGCAGAACTGTATTTACTCCACAGAAGCATT586430.11603118309108512No Hit
GAATGCTGTCTTCTGATGCGAAGATCTAGCAATAGAGGGAGAGTATCACA583570.11546530279225917No Hit
CGAGCCACATGGCAAATGAGGACAGTGGGGAAGAAAGAACAGACAACCCA580960.1149488875545194No Hit
CAGTCTGTCTATGGTCTCCTGGAGCAGGGAGTGGGATGGAGACAATCAAA580290.11481632119080842No Hit
GCACAACTGCCCCTCACAAGAATTCAGCTTCAGTGCAAAATTCATCTGTT577870.11433749939949417No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGCTAGGATTACAG575220.1138131697519806No Hit
TGTTTGATTTGCCTGGGTTGTCTGTTCTTTCTTCCCCACTGTCCTCATTT574870.11374391866645994No Hit
AGAAAACATGACAGATGGCTCTGTGCCCTTTCCCATGAAAGACGACGAGA571850.11314638072853883No Hit
GCGGTGAAAGCGAGGAGACAGGCTGAATTTGAACACTTTCAGAGGAGGTA570660.11291092703776859No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTATAG570280.1128357401449176No Hit
CTGGCCTCGAGATCTGCCTGCCTGGGCCTCCCAAAGTGCTGGGATTACAG569340.11264975151523354No Hit
CTGGCCTCGAGATCTGCCTGTCTCAGCCTCCCAAAGTGCTGGGATTACAG567510.11228766726808267No Hit
GCTGTTTACTTTCCAAAACATTGGCTGAGACATTTATATTTTCATAATCT565310.11185237473052424No Hit
CTGAAGGCCCAATTGACCTGGTCTTTGTGATCGATGGATCCAAGAGTCTT562180.11123307216572521No Hit
AGCAGGAGTTCCCACCAGTTTCTTTCTGAGAACATCTGTTCAGGTTTCTC553380.1094919020154915No Hit
ACCGGGATCTGATGGGTAAGGGTTTCATCTTTAATCTGCAGGAAGGGTGG550560.10893393612643935No Hit
TCTGCTTGTGGTCCACAGTAGTTTTTCGTAAGTATAGGTCCTCATTATAT549380.10870046103811255No Hit
GTCTACGGAGATCTCCAGGAGTGAATTTTTGCGGTCTCATGTTCATCATT545790.10799014276091494No Hit
GACAGGACTAGGTAGGATGATGGGCAGGCAGATATGAGAAGAAGTGATGA544820.10779821832390055No Hit
ACATGACAGCCATCATCAAAGAGATCGTTAGCAGAAACAAAAGGAGATAT544750.10778436810679641No Hit
GCAAGTCCAAACGGATGTGTCATCTGCTCTATATGGCCCAGTCTTCTTCT542580.10735501137656832No Hit
TGCCAGCTTGTCATCACTTCTTCTCATATCTGCCTGCCCATCATCCTACC541410.10712351489068497No Hit
GTGATGATGATGTCTTCAGAACAGGACAGCATCTTCAATGGGGCGGGATC539140.10667437213602243No Hit
GGCTGGACCAGGAATTCCATCACGTCCTTGGTCACCTCTTATACCCATTG538950.10663677868959694No Hit
GCGGTAGAAGGTCCTAAGGTCAGAAAGACAAGAGGGAATCATCCAAAAGA538470.10654180577231145No Hit
GGTAAGTCATATTCTACAAGCAGCTCATAACTATGCCACAGAATGGCTGC536480.10614806388606543No Hit
TCTGTTGGTTGGCGTATGGTTCTTTAGGATGATTGACAGTGGCAGTACCT534530.10576223640959319No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGAATTACAG533420.10554261153837051No Hit
GCATTTCGCTGGTGTGTTCACAATGATGAACCCCTTAACGTTCAAAGAAA527640.10439897932605793No Hit
AATTTTGGGCTCACCCTGTAACTTTAGCCTCATCTATAGCAGTGACACCC520600.10300604320587098No Hit
GGATGGACTGGAGGTGGTCAAGCAGGACGAACTTCTCTTTCAGACCTGGC518500.10259053669274704No Hit
CGAACACCAGGAGATTTTAGGGGATAGCTATATTTTTTTGGCTGTAGACA517930.10247775635347052No Hit
CCTCATCACCTTTTTGCCCAAACATCCCCTGCTGTCCTCTAGGACCTGGT516720.10223834545781338No Hit
TGCCTCTCTGCTGAATGTCCCTGTGGGGGTTTTCTCTGTCAGGCTCTTTT516590.10221262362604858No Hit
GTCATTCAGAAGAACTCTTTTGCCATCTTAAAACTATATCAGAGAAAGAA515920.10208005726233758No Hit
CCGTACAAAGAGCCTTCCGGATTCGGCCAGGTCCTCCTCCTCTTCGTTCT515540.1020048703694866No Hit
CTGGCCTCGAGATCTGCCTTCCTCGGCCTCCCAAAGTGCTGGGATTACAG510600.10102744076242358No Hit
GGAAAGCTGATGGGCATGAATGAGATCACAGAGAAACTCATGCTGCCAGA509990.1009067460133733No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTAGGATTACAG509480.10080583728875749No Hit
ACTTGTGGTCTCCGCAGATACTCAAAGCTGTCAGACCCAGCGAGCTGGCT506560.10022808537527084No Hit
CTGGGGCTTTCCACATACTTGGGCTTCCTGACGGGACCTGTAGTGGGAAA506200.10015685568730674No Hit
AGAAGAAGACTGGGCCATATAGAGCAGATGACACATCCGTTTGGACTTGC506080.10013311245798537No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)