FastQCFastQC Report
Thu 16 May 2024
EGAF00007912901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007912901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49439261
Total Bases6.6 Gbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAG26512075.362553861798217No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAG14165662.8652653202077594No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAG12894422.6081336450397186No Hit
CTGGCCTCGAGATCTGCCTACCTCGGCCTCCCAAAGTGCTGGGATTACAG5364041.0849757645042468No Hit
CTGGCCTCGAGATCTGCCTACCTCAGCCTCCCAAAGTGCTGGGATTACAG3786790.7659479376117697No Hit
CTGGCCTCGAGATCTGCCTACCTTGGCCTCCCAAAGTGCTGGGATTACAG2634140.5328032714728483No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGTTGGGATTACAG1220480.2468645314095613No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTATAG1187590.24021192387968746No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTAGGATTACAG965280.19524563686338273No Hit
CTGGCCTCGAGATCTGCCTGCCTCTGCCTCCCAAAGTGCTGGGATTACAG935850.1892928779821365No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGAGATTACAG933730.1888640689835554No Hit
CTGGCCTCGAGATCTGCCTGTCTCGGCCTCCCAAAGTGCTGGGATTACAG891720.18036677368620052No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTATAG890870.18019484554997697No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAA888050.1796244486745059No Hit
GTGATGATGATGTCTTCAGAACAGGACAGCATCTTCAATGGGACGGGATC864270.17481450622815742No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAGAGTGCTGGGATTACAG850800.17208995094000293No Hit
AGAATCATCTTCAGACAGCCCACTCGCAATATCAGGAGGAGAATCTTCCT848470.17161866557835484No Hit
CTGGCCTCGAGATCTGCCTGCCTTAGCCTCCCAAAGTGCTGGGATTACAG785380.15885755250265574No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCTTCCCAAAGTGCTGGGATTACAG744500.15058882049228042No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAGAGTGCTGGGATTACAG742250.1501337166022769No Hit
TGTGATCCCGTCCCATTGAAGATGCTGTCCTGTTCTGAAGACATCATCAT732510.14816362242955047No Hit
TGTGATCCCGCCCCATTGAAGATGCTGTCCTGTTCTGAAGACATCATCAT724670.1465778382083826No Hit
CTGGCCTCGAGATCTGCCTGCCTCGACCTCCCAAAGTGCTGGGATTACAG724390.14652120305762661No Hit
CTTTCACAGTTACTCAGGCCGAAGAATTCCTGGAAGGTGTTCAGGAGAAT723220.14628454903482477No Hit
GAATGCTGTCTTCTGATGCGAAGATCTAGCAATAGAGGGAGAGTATCACA688750.13931235743997064No Hit
GAGAAGCCTCTAGAAGGGATTCTCTCCTCCTCTGCTTGGCACTTCACACA687920.13914447467165822No Hit
CTGGCCTCGAGATCTGCCTGCCTAGGCCTCCCAAAGTGCTGGGATTACAG669220.13536205567473994No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGAGATTACAG668650.13524676268927238No Hit
AATGACTGTCTCTATTTTCCCGTGTCTTTCAGTAATGTATCCTGCATATG657230.13293685761201002No Hit
CAGTCTGTCTATGGTCTCCTGGAGCAGGGAGTGGGATGGAGACAATCAAA651660.13181022264875683No Hit
AGAAAACATGACAGATGGCTCTGTGCCCTTTCCCATGAAAGACGACGAGA642670.12999182977269824No Hit
CGAGCCACATGGCAAATGAGGACAGTGGGGAAGAAAGAACAGACAACCCA637990.1290452136814909No Hit
AGTGGCCCTGGAATACATCTCCAGTGGAAACCGCAGCTTGTCTGCAGTGG637140.12887328554526734No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGTCTCCCAAAGTGCTGGGATTACAG633340.12810466564215028No Hit
ACTGTCTACGGCCTGGACAAGCAGAGAGTATCCAGACACCTGCCAAGGTA632880.128011622180194No Hit
CTGGCCTCGAGATCTGCCTGCTTCGGCCTCCCAAAGTGCTGGGATTACAG629910.12741088504538933No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGTTGGGATTACAG624570.12633077181311428No Hit
ACATGACAGCCATCATCAAAGAGATCGTTAGCAGAAACAAAAGGAGATAT623160.12604557337537872No Hit
GTCATTCAGAAGAACTCTTTTGCCATCTTAAAACTATATCAGAGAAAGAA617900.12498164161474826No Hit
ATCTAGGGATGGCAGTGACTGGATGTATTCAAAATACTGGTCTAAGGTTT617310.12486230326136954No Hit
ACCTCCCCTAAGTTCTTAAGGCAGAACTGTATTTACTCCACAGAAGCATT616370.12467217096954583No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCTCAAAGTGCTGGGATTACAG614160.1242251578153646No Hit
CTGGCCTCGAGATCTGCCTGCTTTGGCCTCCCAAAGTGCTGGGATTACAG608920.12316527142264524No Hit
TCTCCAGCCTTCCTAGCTTCCCAAAATACAAAGGGAGAAGAATCCTATTG606290.12263330554233001No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGTTGGGATTACAG598070.12097065933085044No Hit
CTGGCCTCGAGATCTGCCTGCTTCAGCCTCCCAAAGTGCTGGGATTACAG597990.12095447785920585No Hit
GTCTACGGAGATCTCCAGGAGTGAATTTTTGCGGTCTCATGTTCATCATT595470.12044476150240191No Hit
GCGGTAGAAGGTCCTAAGGTCAGAAAGACAAGAGGGAATCATCCAAAAGA592160.11977525311310783No Hit
AGGTACATCCAGGCCACACATGCACACAGATGCGTGTCATGCACACCTAC588810.11909765398799145No Hit
AGCAGGAGTTCCCACCAGTTTCTTTCTGAGAACATCTGTTCAGGTTTCTC585110.11834926092443009No Hit
GCGGTGAAAGCGAGGAGACAGGCTGAATTTGAACACTTTCAGAGGAGGTA581680.11765548032766912No Hit
CGAAGTAAAAGGAATTACAACCCTCACTTCCCTTTGAGACAACAAGAGAT580180.11735207773433344No Hit
CAGCTACAATAGACATCCCAGGACTGGGCAGAGCACCCCGTCCCTTTTCA578120.11693540483948577No Hit
AGGTACAGTTCACCACATCAGCATGATCAAGCAGCAATGACGGACAGCTG570880.11547098165565217No Hit
GACAGGACTAGGTAGGATGATGGGCAGGCAGATATGAGAAGAAGTGATGA570110.11531523499107318No Hit
TCTGTTGGTTGGCGTATGGTTCTTTAGGATGATTGACAGTGGCAGTACCT569470.11518578321791663No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTTCCAAAGTGCTGGGATTACAG568600.11500980971378193No Hit
GGTTGTAGCTGTTATTTTGGATTCGGTAGTTTTTTCTGTTGTAGTTGCTG566990.11468415759693495No Hit
GGTAAGTCATATTCTACAAGCAGCTCATAACTATGCCACAGAATGGCTGC566140.1145122294607114No Hit
TGTTTGATTTGCCTGGGTTGTCTGTTCTTTCTTCCCCACTGTCCTCATTT565110.11430389301328756No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAATGCTGGGATTACAG563740.11402678531137429No Hit
GCACAACTGCCCCTCACAAGAATTCAGCTTCAGTGCAAAATTCATCTGTT561530.11357977215719305No Hit
CCGTACAAAGAGCCTTCCGGATTCGGCCAGGTCCTCCTCCTCTTCGTTCT560640.1133997532851472No Hit
GCTGTTTACTTTCCAAAACATTGGCTGAGACATTTATATTTTCATAATCT556190.11249965892491799No Hit
CTGAAGGCCCAATTGACCTGGTCTTTGTGATCGATGGATCCAAGAGTCTT554610.11218007485993774No Hit
ACAGAGGGCTAAGACAGGAGAGGAAATACAAGATTTGCCTCCATGAAGGC552480.11174924317740105No Hit
GGCTGGACCAGGAATTCCATCACGTCCTTGGTCACCTCTTATACCCATTG551900.11163192750797792No Hit
ACCGGGATCTGATGGGTAAGGGTTTCATCTTTAATCTGCAGGAAGGGTGG550720.11139325080122052No Hit
GCAAGTCCAAACGGATGTGTCATCTGCTCTATATGGCCCAGTCTTCTTCT547230.11068733410072615No Hit
TTTCTTTTGGCCTTTAGCTGCTCATGATACTTGTCACAGGTATCTCCTGG546680.11057608648316973No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTATAG543570.10994703177298706No Hit
GCATTAATTCCGGATTTCTGAAAGTTGTGTGTTGGGAATCATTGGCAAAG543330.10989848735805335No Hit
AGAAGAAGACTGGGCCATATAGAGCAGATGACACATCCGTTTGGACTTGC542830.10979735316027478No Hit
TGCCTCTCTGCTGAATGTCCCTGTGGGGGTTTTCTCTGTCAGGCTCTTTT539040.10903075594111329No Hit
CCTCATCACCTTTTTGCCCAAACATCCCCTGCTGTCCTCTAGGACCTGGT538320.10888512269631215No Hit
ACCAACAGCATCATGGTAATGAGCTCCTGTGAAGGGGAACGTTAGAGTCC538140.10884871438511187No Hit
TGACAGATAAGGTTAGATTCGTATGCTTGTCTTTTCCTCTGTCAAAAGAA537090.10863633256977688No Hit
AACCAGGTCCTAGAGGACAGCAGGGGATGTTTGGGCAAAAAGGTGATGAG536490.1085149715324426No Hit
TTGCTGGTCAGCCACATCGCACTGGATGAACAGAGTCTTCTGAGGTTCAA536400.10849676737684245No Hit
TCTGCTTGTGGTCCACAGTAGTTTTTCGTAAGTATAGGTCCTCATTATAT532600.10772814747372539No Hit
CGAACACCAGGAGATTTTAGGGGATAGCTATATTTTTTTGGCTGTAGACA532530.10771398868603639No Hit
CAAGACTCTTGGATCCATCGATCACAAAGACCAGGTCAATTGGGCCTTCA528800.1069595275706083No Hit
AATTTTGGGCTCACCCTGTAACTTTAGCCTCATCTATAGCAGTGACACCC526880.10657117225113863No Hit
CAGATCATCCACCTGCATTGGAGAAGCACGGGACTTATCAGGAGTTGTGA525790.10635069969998136No Hit
TGCCAGCTTGTCATCACTTCTTCTCATATCTGCCTGCCCATCATCCTACC523840.10595627632864496No Hit
CTGGCCTCGAGATCTGCCTGCCTGGGCCTCCCAAAGTGCTGGGATTACAG523230.10583289260735511No Hit
ACTTGTGGTCTCCGCAGATACTCAAAGCTGTCAGACCCAGCGAGCTGGCT519650.1051087717512606No Hit
CAAAAGGATCTCAGCAACCCCAAAGAAGTGGAAGAAGAATATCTGAGTCA519520.10508247685983817No Hit
CTGAACCGCACTAAACCATTGTTGTTCGTTGTGCCATTGAAAAAACGCAC519320.10504202318072674No Hit
CATCACTTCCAAAGGGCTCACTACAGGGAGGAAGACTCTGAAACTTGTTC517070.10458691929072321No Hit
GCATTTCGCTGGTGTGTTCACAATGATGAACCCCTTAACGTTCAAAGAAA516230.10441701383845524No Hit
CCAGGAGTGGAATATGATGGCATATGACAAAGAGCTTAGACCCGACAACA515360.10424104033432054No Hit
ACAGTTCAGACACTGATACGTGACTGTCAAGTTATCAGAGAGACTAAAGA514470.10406102146227468No Hit
CTGGGGCTTTCCACATACTTGGGCTTCCTGACGGGACCTGTAGTGGGAAA514360.10403877193876342No Hit
TGTTCTTATTAGCACATGGTACAGGGTTGACAGAGGGATAACCAGGGAGT513760.10391741090142913No Hit
GTCACAAATCTGGAAGTATTATTTTGCTAAAACGTTTGAATTTAGTATTT512100.1035816453648043No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGAATTACAG511970.10355535047338188No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGCTAGGATTACAG511550.10347039774724787No Hit
CGTTGACGTTCCAGGAGATGGTGGGCCGGGGGTGCCCTGACGCTTCACAA510610.10328026545542418No Hit
CTGTAGCTCCAGTTCCTCTGTGTGTGCACAGAAGGAAGGAAGCGCTACAC510300.10321756225280147No Hit
TCCTGATGAGAATAATATTGCAAGGCTTCAGGAAGAACTCATTGCTGTGA509370.10302945264493335No Hit
AAGGTTCTGGCTGTTTTCTACACCATCCTCACCCCAATGCTCAACCCCAT507960.1027442542071978No Hit
CTTTGAACGATGTCTTCCCATACTCCGCACCCTTCAGTCTCTGGTAACTA507920.10273616347137551No Hit
GTGATGATGATGTCTTCAGAACAGGACAGCATCTTCAATGGGGCGGGATC506620.10247321455715125No Hit
GGATGGACTGGAGGTGGTCAAGCAGGACGAACTTCTCTTTCAGACCTGGC506410.10243073819408426No Hit
TTCTGCTCCACAGAGTTGGGTCCTCAGGTCATGCCCAACAGGCCAGTGAC503250.10179157006412375No Hit
TGGCAGGTCTACCTCCACTCCCTGCAGCGCGTGGATGTCTCCTGCGTCGC502710.10168234513052289No Hit
GTACACACTCTGAGACAACCTGTTGTCGGGTCTAAGCTCTTTGTCATATG501040.10134455690994248No Hit
CTCTCTGGCTAAAGTAGGTGCTCAAATGCTTCTCTCTGGGGCTCTCCAAA500510.10123735466029721No Hit
TCCTAACCTGACCATCACAGACGACTGTGACACAATCACAAGGGTAGAGA499590.10105126773638465No Hit
ACTAGCAATTCCTGTACACATCTCACAGCTGGGTGAGGATGATCAAGGTG499280.10098856453376194No Hit
TCTCACCGCAAACACAGCAACCGGAGGCTGGGCGAGTTTGTTCTCCTTTT498950.1009218159632281No Hit
AGACAATGAGAATGACCCAAAGGCTATTTTTCTCAAAGGAGTGATGCGTG498780.1008874303359834No Hit
AGCCAATGTGATACTCTCCCTCTATTGCTAGATCTTCGCATCAGAAGACA497540.10063661752549255No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)