FastQCFastQC Report
Tue 23 May 2023
EGAF00007912901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007912901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49439261
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAG26419095.343746946379316No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAG14115592.8551377416422143No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAG12850122.599173155116538No Hit
CTGGCCTCGAGATCTGCCTACCTCGGCCTCCCAAAGTGCTGGGATTACAG5345921.0813106611767518No Hit
CTGGCCTCGAGATCTGCCTACCTCAGCCTCCCAAAGTGCTGGGATTACAG3774270.7634155372993945No Hit
CTGGCCTCGAGATCTGCCTACCTTGGCCTCCCAAAGTGCTGGGATTACAG2624830.5309201527102114No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGTTGGGATTACAG1216760.24611209297808878No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTATAG1175610.23778874850091306No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTAGGATTACAG961280.19443656328115422No Hit
CTGGCCTCGAGATCTGCCTGCCTCTGCCTCCCAAAGTGCTGGGATTACAG931840.18848178171595242No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGAGATTACAG929800.1880691541890159No Hit
CTGGCCTCGAGATCTGCCTGTCTCGGCCTCCCAAAGTGCTGGGATTACAG889010.17981862633424073No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTATAG885400.1790884374262795No Hit
GTGATGATGATGTCTTCAGAACAGGACAGCATCTTCAATGGGACGGGATC861350.17422388251313062No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAGAGTGCTGGGATTACAG846800.17128087735777442No Hit
AGAATCATCTTCAGACAGCCCACTCGCAATATCAGGAGGAGAATCTTCCT846500.17122019683910727No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAA793370.16047367698315718No Hit
CTGGCCTCGAGATCTGCCTGCCTTAGCCTCCCAAAGTGCTGGGATTACAG782720.15831951857047377No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCTTCCCAAAGTGCTGGGATTACAG742520.1501883290690773No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAGAGTGCTGGGATTACAG740690.14981817790520777No Hit
TGTGATCCCGTCCCATTGAAGATGCTGTCCTGTTCTGAAGACATCATCAT731560.14797146745377118No Hit
TGTGATCCCGCCCCATTGAAGATGCTGTCCTGTTCTGAAGACATCATCAT723580.14635736565722532No Hit
CTGGCCTCGAGATCTGCCTGCCTCGACCTCCCAAAGTGCTGGGATTACAG722170.1460721672194898No Hit
CTTTCACAGTTACTCAGGCCGAAGAATTCCTGGAAGGTGTTCAGGAGAAT721860.14600946401686707No Hit
GAGAAGCCTCTAGAAGGGATTCTCTCCTCCTCTGCTTGGCACTTCACACA686670.1388916391772118No Hit
GAATGCTGTCTTCTGATGCGAAGATCTAGCAATAGAGGGAGAGTATCACA684920.13853766948498683No Hit
CTGGCCTCGAGATCTGCCTGCCTAGGCCTCCCAAAGTGCTGGGATTACAG666620.13483615784629144No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGAGATTACAG666340.13477952269553545No Hit
AATGACTGTCTCTATTTTCCCGTGTCTTTCAGTAATGTATCCTGCATATG656850.1328599956216983No Hit
CAGTCTGTCTATGGTCTCCTGGAGCAGGGAGTGGGATGGAGACAATCAAA651280.13173336065844513No Hit
AGAAAACATGACAGATGGCTCTGTGCCCTTTCCCATGAAAGACGACGAGA641530.12976124380176313No Hit
CGAGCCACATGGCAAATGAGGACAGTGGGGAAGAAAGAACAGACAACCCA635780.12859820052730966No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGTCTCCCAAAGTGCTGGGATTACAG630710.12757269976183505No Hit
ACTGTCTACGGCCTGGACAAGCAGAGAGTATCCAGACACCTGCCAAGGTA630090.1274472933565896No Hit
AGTGGCCCTGGAATACATCTCCAGTGGAAACCGCAGCTTGTCTGCAGTGG628400.12710545976809806No Hit
CTGGCCTCGAGATCTGCCTGCTTCGGCCTCCCAAAGTGCTGGGATTACAG627330.12688903258485196No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGTTGGGATTACAG622740.12596062064924474No Hit
ACATGACAGCCATCATCAAAGAGATCGTTAGCAGAAACAAAAGGAGATAT622600.12593230307386674No Hit
GTCATTCAGAAGAACTCTTTTGCCATCTTAAAACTATATCAGAGAAAGAA616230.12464385339416785No Hit
ACCTCCCCTAAGTTCTTAAGGCAGAACTGTATTTACTCCACAGAAGCATT616120.12462160387065656No Hit
ATCTAGGGATGGCAGTGACTGGATGTATTCAAAATACTGGTCTAAGGTTT615980.12459328629527855No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCTCAAAGTGCTGGGATTACAG612280.12384489323171718No Hit
CTGGCCTCGAGATCTGCCTGCTTTGGCCTCCCAAAGTGCTGGGATTACAG607120.12280118831064243No Hit
TCTCCAGCCTTCCTAGCTTCCCAAAATACAAAGGGAGAAGAATCCTATTG601970.12175950607352323No Hit
CTGGCCTCGAGATCTGCCTGCTTCAGCCTCCCAAAGTGCTGGGATTACAG596420.12063691647818117No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGTTGGGATTACAG596200.12059241743115862No Hit
GTCTACGGAGATCTCCAGGAGTGAATTTTTGCGGTCTCATGTTCATCATT592960.11993706782955352No Hit
AGGTACATCCAGGCCACACATGCACACAGATGCGTGTCATGCACACCTAC587440.11882054628607819No Hit
GCGGTAGAAGGTCCTAAGGTCAGAAAGACAAGAGGGAATCATCCAAAAGA586210.11857175615954292No Hit
AGCAGGAGTTCCCACCAGTTTCTTTCTGAGAACATCTGTTCAGGTTTCTC583840.11809238006207252No Hit
GCGGTGAAAGCGAGGAGACAGGCTGAATTTGAACACTTTCAGAGGAGGTA579950.11730555600335531No Hit
CGAAGTAAAAGGAATTACAACCCTCACTTCCCTTTGAGACAACAAGAGAT579470.1172084671734879No Hit
CAGCTACAATAGACATCCCAGGACTGGGCAGAGCACCCCGTCCCTTTTCA575560.11641759774685952No Hit
AGGTACAGTTCACCACATCAGCATGATCAAGCAGCAATGACGGACAGCTG569400.11517162443022762No Hit
TCTGTTGGTTGGCGTATGGTTCTTTAGGATGATTGACAGTGGCAGTACCT569160.11512308001529392No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTTCCAAAGTGCTGGGATTACAG567050.11469629370066839No Hit
GACAGGACTAGGTAGGATGATGGGCAGGCAGATATGAGAAGAAGTGATGA566870.11465988538946811No Hit
TGTTTGATTTGCCTGGGTTGTCTGTTCTTTCTTCCCCACTGTCCTCATTT563990.11407735241026357No Hit
GGTAAGTCATATTCTACAAGCAGCTCATAACTATGCCACAGAATGGCTGC563610.11400049041995186No Hit
GGTTGTAGCTGTTATTTTGGATTCGGTAGTTTTTTCTGTTGTAGTTGCTG563240.11392565111359573No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAATGCTGGGATTACAG561460.11356561336950403No Hit
GCACAACTGCCCCTCACAAGAATTCAGCTTCAGTGCAAAATTCATCTGTT561060.11348470601128119No Hit
CCGTACAAAGAGCCTTCCGGATTCGGCCAGGTCCTCCTCCTCTTCGTTCT560200.11331075519110208No Hit
GCTGTTTACTTTCCAAAACATTGGCTGAGACATTTATATTTTCATAATCT556000.11246122792976214No Hit
CTGAAGGCCCAATTGACCTGGTCTTTGTGATCGATGGATCCAAGAGTCTT554180.11209309944984816No Hit
GGCTGGACCAGGAATTCCATCACGTCCTTGGTCACCTCTTATACCCATTG551400.11153079331019936No Hit
ACAGAGGGCTAAGACAGGAGAGGAAATACAAGATTTGCCTCCATGAAGGC549340.11111412041535168No Hit
GCAAGTCCAAACGGATGTGTCATCTGCTCTATATGGCCCAGTCTTCTTCT546810.11060238137459216No Hit
ACCGGGATCTGATGGGTAAGGGTTTCATCTTTAATCTGCAGGAAGGGTGG546360.11051136059659145No Hit
AGAAGAAGACTGGGCCATATAGAGCAGATGACACATCCGTTTGGACTTGC541900.10960924355240666No Hit
GCATTAATTCCGGATTTCTGAAAGTTGTGTGTTGGGAATCATTGGCAAAG541480.10952429082627267No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTATAG541180.10946361030760553No Hit
TTTCTTTTGGCCTTTAGCTGCTCATGATACTTGTCACAGGTATCTCCTGG540300.10928561411951525No Hit
TGCCTCTCTGCTGAATGTCCCTGTGGGGGTTTTCTCTGTCAGGCTCTTTT538100.10884062364928959No Hit
ACCAACAGCATCATGGTAATGAGCTCCTGTGAAGGGGAACGTTAGAGTCC537060.10863026451791016No Hit
AACCAGGTCCTAGAGGACAGCAGGGGATGTTTGGGCAAAAAGGTGATGAG535880.10839158781115277No Hit
CCTCATCACCTTTTTGCCCAAACATCCCCTGCTGTCCTCTAGGACCTGGT535180.10824999993426278No Hit
TTGCTGGTCAGCCACATCGCACTGGATGAACAGAGTCTTCTGAGGTTCAA535100.10823381846261819No Hit
TGACAGATAAGGTTAGATTCGTATGCTTGTCTTTTCCTCTGTCAAAAGAA534990.10821156893910693No Hit
TCTGCTTGTGGTCCACAGTAGTTTTTCGTAAGTATAGGTCCTCATTATAT531710.10754812860167955No Hit
CGAACACCAGGAGATTTTAGGGGATAGCTATATTTTTTTGGCTGTAGACA528200.10683816653327402No Hit
CAAGACTCTTGGATCCATCGATCACAAAGACCAGGTCAATTGGGCCTTCA526920.10657926298696091No Hit
AATTTTGGGCTCACCCTGTAACTTTAGCCTCATCTATAGCAGTGACACCC526300.1064538565817155No Hit
CAGATCATCCACCTGCATTGGAGAAGCACGGGACTTATCAGGAGTTGTGA525250.1062414747663805No Hit
CTGGCCTCGAGATCTGCCTGCCTGGGCCTCCCAAAGTGCTGGGATTACAG521210.10542431044832973No Hit
ACTTGTGGTCTCCGCAGATACTCAAAGCTGTCAGACCCAGCGAGCTGGCT518940.10496516119041503No Hit
TGCCAGCTTGTCATCACTTCTTCTCATATCTGCCTGCCCATCATCCTACC518160.10480739184188048No Hit
CTGAACCGCACTAAACCATTGTTGTTCGTTGTGCCATTGAAAAAACGCAC516470.10446555825338893No Hit
GCATTTCGCTGGTGTGTTCACAATGATGAACCCCTTAACGTTCAAAGAAA516070.10438465089516609No Hit
CCAGGAGTGGAATATGATGGCATATGACAAAGAGCTTAGACCCGACAACA514240.10401449973129655No Hit
CATCACTTCCAAAGGGCTCACTACAGGGAGGAAGACTCTGAAACTTGTTC513920.10394977384471826No Hit
CTGGGGCTTTCCACATACTTGGGCTTCCTGACGGGACCTGTAGTGGGAAA513800.10392550163725142No Hit
CAAAAGGATCTCAGCAACCCCAAAGAAGTGGAAGAAGAATATCTGAGTCA513190.10380211791596156No Hit
TGTTCTTATTAGCACATGGTACAGGGTTGACAGAGGGATAACCAGGGAGT513070.10377784570849471No Hit
GTCACAAATCTGGAAGTATTATTTTGCTAAAACGTTTGAATTTAGTATTT510500.1032580159319129No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGCTAGGATTACAG509680.10309215584755604No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGAATTACAG509340.10302338459306665No Hit
CTGTAGCTCCAGTTCCTCTGTGTGTGCACAGAAGGAAGGAAGCGCTACAC509040.1029627040743995No Hit
AAGGTTCTGGCTGTTTTCTACACCATCCTCACCCCAATGCTCAACCCCAT507480.10264716537733039No Hit
CTTTGAACGATGTCTTCCCATACTCCGCACCCTTCAGTCTCTGGTAACTA506530.1024550104015511No Hit
ACAGTTCAGACACTGATACGTGACTGTCAAGTTATCAGAGAGACTAAAGA505700.10228712763323869No Hit
GTGATGATGATGTCTTCAGAACAGGACAGCATCTTCAATGGGGCGGGATC505260.10219812953919356No Hit
GGATGGACTGGAGGTGGTCAAGCAGGACGAACTTCTCTTTCAGACCTGGC504030.1019493394126583No Hit
TCCTGATGAGAATAATATTGCAAGGCTTCAGGAAGAACTCATTGCTGTGA503840.10191090841750244No Hit
CGTTGACGTTCCAGGAGATGGTGGGCCGGGGGTGCCCTGACGCTTCACAA502690.10167829976261175No Hit
TTCTGCTCCACAGAGTTGGGTCCTCAGGTCATGCCCAACAGGCCAGTGAC500930.10132230738643119No Hit
TGGCAGGTCTACCTCCACTCCCTGCAGCGCGTGGATGTCTCCTGCGTCGC500310.10119690098118579No Hit
GTACACACTCTGAGACAACCTGTTGTCGGGTCTAAGCTCTTTGTCATATG499980.10113015241065193No Hit
CTCTCTGGCTAAAGTAGGTGCTCAAATGCTTCTCTCTGGGGCTCTCCAAA499790.10109172141549608No Hit
ACTAGCAATTCCTGTACACATCTCACAGCTGGGTGAGGATGATCAAGGTG498780.1008874303359834No Hit
AGACAATGAGAATGACCCAAAGGCTATTTTTCTCAAAGGAGTGATGCGTG498060.10074179709118225No Hit
TCTCACCGCAAACACAGCAACCGGAGGCTGGGCGAGTTTGTTCTCCTTTT498020.10073370635535997No Hit
TCCTAACCTGACCATCACAGACGACTGTGACACAATCACAAGGGTAGAGA497150.10055773285122527No Hit
AGCCAATGTGATACTCTCCCTCTATTGCTAGATCTTCGCATCAGAAGACA496470.10042019034224642No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)