FastQCFastQC Report
Sun 5 May 2024
EGAF00007912903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007912903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35060844
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAG20972745.981812645468546No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAG10987863.133940529212588No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAG10000262.8522587762005958No Hit
CTGGCCTCGAGATCTGCCTACCTCGGCCTCCCAAAGTGCTGGGATTACAG6216391.773029194619502No Hit
CTGGCCTCGAGATCTGCCTACCTCAGCCTCCCAAAGTGCTGGGATTACAG4259291.2148281427566319No Hit
CTGGCCTCGAGATCTGCCTACCTTGGCCTCCCAAAGTGCTGGGATTACAG3251550.9274020899211668No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTATAG1065780.3039801323664656No Hit
CTGGCCTCGAGATCTGCCTGTCTCGGCCTCCCAAAGTGCTGGGATTACAG943840.26920059311749595No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGTTGGGATTACAG877650.25032198312168413No Hit
CTGGCCTCGAGATCTGCCTGCCTCTGCCTCCCAAAGTGCTGGGATTACAG826740.2358015112243162No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTAGGATTACAG782090.223066506898693No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTATAG719690.20526887487363393No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGAGATTACAG712850.203317980593964No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAA654210.18659276998579954No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAGAGTGCTGGGATTACAG605300.17264273501231173No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCTTCCCAAAGTGCTGGGATTACAG583650.1664677553113097No Hit
CTGGCCTCGAGATCTGCCTGCCTTAGCCTCCCAAAGTGCTGGGATTACAG564570.16102578705749354No Hit
CTGGCCTCGAGATCTGCCTGTCTTGGCCTCCCAAAGTGCTGGGATTACAG556550.15873833499273435No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAGAGTGCTGGGATTACAG552340.15753756526796675No Hit
CTGGCCTCGAGATCTGCCTGCTTCGGCCTCCCAAAGTGCTGGGATTACAG537790.15338763664673902No Hit
CTGGCCTCGAGATCTGCCTGCCTCGACCTCCCAAAGTGCTGGGATTACAG533740.15223250187588183No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGTCTCCCAAAGTGCTGGGATTACAG522670.14907513350220547No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGAGATTACAG520880.1485645924553328No Hit
AGAATCATCTTCAGACAGCCCACTCGCAATATCAGGAGGAGAATCTTCCT517180.1475092841461546No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCTCAAAGTGCTGGGATTACAG499730.14253222198530074No Hit
TGTGATCCCGTCCCATTGAAGATGCTGTCCTGTTCTGAAGACATCATCAT491560.14020198715125057No Hit
GTGATGATGATGTCTTCAGAACAGGACAGCATCTTCAATGGGACGGGATC490080.13977986382757926No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTTCCAAAGTGCTGGGATTACAG485780.13855342444123706No Hit
TGTGATCCCGCCCCATTGAAGATGCTGTCCTGTTCTGAAGACATCATCAT485320.13842222394874465No Hit
CTGGCCTCGAGATCTGCCTGCCTAGGCCTCCCAAAGTGCTGGGATTACAG470380.13416106012736032No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGTTGGGATTACAG465940.13289469015634647No Hit
CTTTCACAGTTACTCAGGCCGAAGAATTCCTGGAAGGTGTTCAGGAGAAT459170.13096376116901237No Hit
CTGGCCTCGAGATCTGCCTTCCTCGGCCTCCCAAAGTGCTGGGATTACAG453360.12930664190514066No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGTTGGGATTACAG450520.12849662147323093No Hit
CTGGCCTCGAGATCTGCCTGCTTTGGCCTCCCAAAGTGCTGGGATTACAG448660.12796611513402245No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCGAAGTGCTGGGATTACAG446180.12725877334841112No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAATGCTGGGATTACAG434890.12403865691310798No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGCTAGGATTACAG434510.12393027389757075No Hit
CTGGCCTCGAGATCTGCCTGTCTCAGCCTCCCAAAGTGCTGGGATTACAG432430.12333701949673545No Hit
CTGGCCTCGAGATCTGCCTGCTTCAGCCTCCCAAAGTGCTGGGATTACAG431760.12314592312723562No Hit
CTGGCCTCGAGATCTGCCTGCCTGGGCCTCCCAAAGTGCTGGGATTACAG418340.11931829136800015No Hit
GAGAAGCCTCTAGAAGGGATTCTCTCCTCCTCTGCTTGGCACTTCACACA413730.11800343425845652No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTATAG404940.11549636397800349No Hit
CTGGCCTCGAGATCTGCCTATCTCGGCCTCCCAAAGTGCTGGGATTACAG403010.1149458923464592No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGAATTACAG399190.11385635782184822No Hit
CTGAAGGCCCAATTGACCTGGTCTTTGTGATCGATGGATCCAAGAGTCTT394060.11239318711209575No Hit
TCTGCTTGTGGTCCACAGTAGTTTTTCGTAAGTATAGGTCCTCATTATAT384080.10954670686193406No Hit
GCGGTGAAAGCGAGGAGACAGGCTGAATTTGAACACTTTCAGAGGAGGTA383450.10936701923091184No Hit
TCTGTTGGTTGGCGTATGGTTCTTTAGGATGATTGACAGTGGCAGTACCT382210.10901334833810618No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTAGGATTACAG379720.10830315436787545No Hit
AGGTACATCCAGGCCACACATGCACACAGATGCGTGTCATGCACACCTAC376050.1072564026125555No Hit
AATGACTGTCTCTATTTTCCCGTGTCTTTCAGTAATGTATCCTGCATATG375120.10699114944295122No Hit
GAATGCTGTCTTCTGATGCGAAGATCTAGCAATAGAGGGAGAGTATCACA373110.10641786033445175No Hit
GCTGTTTACTTTCCAAAACATTGGCTGAGACATTTATATTTTCATAATCT373000.10638648630363834No Hit
AGTGGCCCTGGAATACATCTCCAGTGGAAACCGCAGCTTGTCTGCAGTGG369130.10528269085593032No Hit
CGAGCCACATGGCAAATGAGGACAGTGGGGAAGAAAGAACAGACAACCCA368500.1051030032249081No Hit
CGAAGTAAAAGGAATTACAACCCTCACTTCCCTTTGAGACAACAAGAGAT364170.10386800728470769No Hit
CAGTCTGTCTATGGTCTCCTGGAGCAGGGAGTGGGATGGAGACAATCAAA363390.10364553688439444No Hit
GCACAACTGCCCCTCACAAGAATTCAGCTTCAGTGCAAAATTCATCTGTT361910.10322341356072318No Hit
GCAAGTCCAAACGGATGTGTCATCTGCTCTATATGGCCCAGTCTTCTTCT357360.10192566955889595No Hit
ACTGTCTACGGCCTGGACAAGCAGAGAGTATCCAGACACCTGCCAAGGTA356150.10158055521994849No Hit
TGTTTGATTTGCCTGGGTTGTCTGTTCTTTCTTCCCCACTGTCCTCATTT352590.10056517749544192No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)