FastQCFastQC Report
Tue 23 May 2023
EGAF00007912903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007912903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35060844
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAG20898535.960646583407975No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAG10949393.1229681749817546No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAG9965502.84234458246356No Hit
CTGGCCTCGAGATCTGCCTACCTCGGCCTCCCAAAGTGCTGGGATTACAG6195991.7672107379959252No Hit
CTGGCCTCGAGATCTGCCTACCTCAGCCTCCCAAAGTGCTGGGATTACAG4245151.2107951537047996No Hit
CTGGCCTCGAGATCTGCCTACCTTGGCCTCCCAAAGTGCTGGGATTACAG3240660.9242960608706395No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTATAG1053660.3005232846077522No Hit
CTGGCCTCGAGATCTGCCTGTCTCGGCCTCCCAAAGTGCTGGGATTACAG940510.26825081563923564No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGTTGGGATTACAG874940.2495490410898266No Hit
CTGGCCTCGAGATCTGCCTGCCTCTGCCTCCCAAAGTGCTGGGATTACAG823230.23480039442290665No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTAGGATTACAG778930.22216521655896246No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTATAG715970.20420786219521697No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGAGATTACAG710340.20260208225449447No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAGAGTGCTGGGATTACAG603010.17198958473446901No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAA582550.16615401500317564No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCTTCCCAAAGTGCTGGGATTACAG582140.1660370754337802No Hit
CTGGCCTCGAGATCTGCCTGCCTTAGCCTCCCAAAGTGCTGGGATTACAG562870.16054091567219544No Hit
CTGGCCTCGAGATCTGCCTGTCTTGGCCTCCCAAAGTGCTGGGATTACAG554760.15822779394586164No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAGAGTGCTGGGATTACAG551720.1573607298215639No Hit
CTGGCCTCGAGATCTGCCTGCTTCGGCCTCCCAAAGTGCTGGGATTACAG536020.15288279996910512No Hit
CTGGCCTCGAGATCTGCCTGCCTCGACCTCCCAAAGTGCTGGGATTACAG532300.15182178729068815No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGTCTCCCAAAGTGCTGGGATTACAG520730.14852180968604178No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGAGATTACAG518980.1480226773776467No Hit
AGAATCATCTTCAGACAGCCCACTCGCAATATCAGGAGGAGAATCTTCCT515290.14697022125308792No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCTCAAAGTGCTGGGATTACAG498120.14207302026157728No Hit
TGTGATCCCGTCCCATTGAAGATGCTGTCCTGTTCTGAAGACATCATCAT491170.14009075195109394No Hit
GTGATGATGATGTCTTCAGAACAGGACAGCATCTTCAATGGGACGGGATC488340.1392835837038036No Hit
TGTGATCCCGCCCCATTGAAGATGCTGTCCTGTTCTGAAGACATCATCAT484930.13831098874858805No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTTCCAAAGTGCTGGGATTACAG484540.1381997535484314No Hit
CTGGCCTCGAGATCTGCCTGCCTAGGCCTCCCAAAGTGCTGGGATTACAG468380.13359062320348022No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGTTGGGATTACAG464770.13256098455587664No Hit
CTTTCACAGTTACTCAGGCCGAAGAATTCCTGGAAGGTGTTCAGGAGAAT458600.13080118664570653No Hit
CTGGCCTCGAGATCTGCCTTCCTCGGCCTCCCAAAGTGCTGGGATTACAG451770.128853144550656No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGTTGGGATTACAG449210.12812298528808946No Hit
CTGGCCTCGAGATCTGCCTGCTTTGGCCTCCCAAAGTGCTGGGATTACAG447190.12754684399497057No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCGAAGTGCTGGGATTACAG445230.12698781580956808No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAATGCTGGGATTACAG433450.1236279423279143No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGCTAGGATTACAG433060.12351670712775768No Hit
CTGGCCTCGAGATCTGCCTGTCTCAGCCTCCCAAAGTGCTGGGATTACAG430630.12282362626524336No Hit
CTGGCCTCGAGATCTGCCTGCTTCAGCCTCCCAAAGTGCTGGGATTACAG430580.12280936534214636No Hit
CTGGCCTCGAGATCTGCCTGCCTGGGCCTCCCAAAGTGCTGGGATTACAG417210.1189959945060079No Hit
GAGAAGCCTCTAGAAGGGATTCTCTCCTCCTCTGCTTGGCACTTCACACA412880.11776099856580749No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTATAG402730.11486603117711598No Hit
CTGGCCTCGAGATCTGCCTATCTCGGCCTCCCAAAGTGCTGGGATTACAG401590.11454088213050434No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGAATTACAG397370.11333726022111731No Hit
CTGAAGGCCCAATTGACCTGGTCTTTGTGATCGATGGATCCAAGAGTCTT393480.11222776040417054No Hit
TCTGCTTGTGGTCCACAGTAGTTTTTCGTAAGTATAGGTCCTCATTATAT383480.10937557578477004No Hit
GCGGTGAAAGCGAGGAGACAGGCTGAATTTGAACACTTTCAGAGGAGGTA382440.10907894858435238No Hit
TCTGTTGGTTGGCGTATGGTTCTTTAGGATGATTGACAGTGGCAGTACCT381860.10891352187642717No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTAGGATTACAG378050.10782683953643557No Hit
AGGTACATCCAGGCCACACATGCACACAGATGCGTGTCATGCACACCTAC375410.10707386279691383No Hit
AATGACTGTCTCTATTTTCCCGTGTCTTTCAGTAATGTATCCTGCATATG374810.10690273171974983No Hit
GCTGTTTACTTTCCAAAACATTGGCTGAGACATTTATATTTTCATAATCT372690.1062980685804369No Hit
GAATGCTGTCTTCTGATGCGAAGATCTAGCAATAGAGGGAGAGTATCACA371200.10587309307214625No Hit
CGAGCCACATGGCAAATGAGGACAGTGGGGAAGAAAGAACAGACAACCCA366890.10464380150118462No Hit
AGTGGCCCTGGAATACATCTCCAGTGGAAACCGCAGCTTGTCTGCAGTGG365730.10431294808533417No Hit
CGAAGTAAAAGGAATTACAACCCTCACTTCCCTTTGAGACAACAAGAGAT363830.10377103300764808No Hit
CAGTCTGTCTATGGTCTCCTGGAGCAGGGAGTGGGATGGAGACAATCAAA363380.10364268469977506No Hit
GCACAACTGCCCCTCACAAGAATTCAGCTTCAGTGCAAAATTCATCTGTT361620.10314070020676057No Hit
GCAAGTCCAAACGGATGTGTCATCTGCTCTATATGGCCCAGTCTTCTTCT357090.10184866057417213No Hit
ACTGTCTACGGCCTGGACAAGCAGAGAGTATCCAGACACCTGCCAAGGTA353740.10089317872667299No Hit
TGTTTGATTTGCCTGGGTTGTCTGTTCTTTCTTCCCCACTGTCCTCATTT352350.10049672506457631No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)