FastQCFastQC Report
Mon 13 May 2024
EGAF00007912905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007912905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63500210
Total Bases8.8 Gbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAG35872305.649162420092784No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAG18378022.8941668066924504No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAG17198792.708461909023608No Hit
CTGGCCTCGAGATCTGCCTACCTCGGCCTCCCAAAGTGCTGGGATTACAG9886121.5568641426540164No Hit
CTGGCCTCGAGATCTGCCTACCTCAGCCTCCCAAAGTGCTGGGATTACAG6945801.0938231542856316No Hit
CTGGCCTCGAGATCTGCCTACCTTGGCCTCCCAAAGTGCTGGGATTACAG5360050.8440995706943332No Hit
GTCAGATGATCTTCCCAGCATCAGTACACGAAGTTCAGCCTGCACGTGCC2100940.330855598745264No Hit
GCCTCACGAGAACAGCTCTCTTGCCCCATGGCCGGACCTCCCCACCCTGG1720120.27088414353275364No Hit
CTGGCCTCGAGATCTGCCTGTCTCGGCCTCCCAAAGTGCTGGGATTACAG1545310.24335510071541497No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGTTGGGATTACAG1505420.23707323172632028No Hit
CTGGCCTCGAGATCTGCCTGCCTCTGCCTCCCAAAGTGCTGGGATTACAG1411280.2222480839039745No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTAGGATTACAG1363810.21477251807513706No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTATAG1346700.21207803879703702No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTATAG1219030.19197259347646253No Hit
TGTAAAACTCTTATTGCTGGACAGAAATCTGCTCGTCTTGATCTACTTTA1218400.1918733812061409No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAA1192780.18783874887972812No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGAGATTACAG1183700.18640883234874342No Hit
GCACGTGCAGGCTGAACTTCGTGTACTGATGCTGGGAAGATCATCTGACC1085710.17097738731887657No Hit
CTGGCCTCGAGATCTGCCTGCCTTAGCCTCCCAAAGTGCTGGGATTACAG1011050.15921994588679314No Hit
AGAATCATCTTCAGACAGCCCACTCGCAATATCAGGAGGAGAATCTTCCT994280.15657900973870795No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAGAGTGCTGGGATTACAG978610.15411130136420023No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCTTCCCAAAGTGCTGGGATTACAG968050.15244831473785678No Hit
CTGGCCTCGAGATCTGCCTGTCTTGGCCTCCCAAAGTGCTGGGATTACAG965760.15208768600922737No Hit
GAGATTCCGGCCTCTGCAACCGTGGGGCATGCGGTGGAGGGGTGGCCCCT951770.14988454368891063No Hit
CCACTGGTGTTGGCGGTATTGGGCGCCAGGGTGGGGAGGTCCGGCCATGG915750.14421212150322024No Hit
CTGGCCTCGAGATCTGCCTGCCTCGACCTCCCAAAGTGCTGGGATTACAG895540.14102945486322013No Hit
GCAGGCGATAAAGTAGATCAAGACGAGCAGATTTCTGTCCAGCAATAAGA874600.1377318279734823No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGAGATTACAG863540.1359901014500582No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGTCTCCCAAAGTGCTGGGATTACAG860090.1354467961602017No Hit
CTGAAGGCCCAATTGACCTGGTCTTTGTGATCGATGGATCCAAGAGTCTT859270.1353176627289894No Hit
GCGGTAGAAGGTCCTAAGGTCAGAAAGACAAGAGGGAATCATCCAAAAGA851810.13414286346454604No Hit
CTGGCCTCGAGATCTGCCTGCTTCGGCCTCCCAAAGTGCTGGGATTACAG844150.1329365682412704No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTTCCAAAGTGCTGGGATTACAG811110.12773343584218066No Hit
CTGGCCTCGAGATCTGCCTGCTTTGGCCTCCCAAAGTGCTGGGATTACAG807580.12717753216879127No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGTTGGGATTACAG794520.12512084605704454No Hit
AATGACTGTCTCTATTTTCCCGTGTCTTTCAGTAATGTATCCTGCATATG791680.12467360344162641No Hit
GCACAACTGCCCCTCACAAGAATTCAGCTTCAGTGCAAAATTCATCTGTT789010.12425313239121571No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGTTGGGATTACAG770790.12138385054159663No Hit
GCGGTGAAAGCGAGGAGACAGGCTGAATTTGAACACTTTCAGAGGAGGTA770750.12137755134983019No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAGAGTGCTGGGATTACAG758960.11952086457666833No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAATGCTGGGATTACAG753440.11865157611289791No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCGAAGTGCTGGGATTACAG752370.11848307273314529No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCTCAAAGTGCTGGGATTACAG749730.11806732607655944No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTATAG745710.11743425730403095No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAATGCTGGGATTACAG741210.11672559823030507No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGCTAGGATTACAG735040.11575394790032976No Hit
CTGGCCTCGAGATCTGCCTGCCTAGGCCTCCCAAAGTGCTGGGATTACAG723410.11392245789423373No Hit
ACTGTCTACGGCCTGGACAAGCAGAGAGTATCCAGACACCTGCCAAGGTA719360.11328466472788042No Hit
ATCTAGGGATGGCAGTGACTGGATGTATTCAAAATACTGGTCTAAGGTTT718190.11310041336871168No Hit
CTGGCCTCGAGATCTGCCTGTCTCAGCCTCCCAAAGTGCTGGGATTACAG714690.11254923408914712No Hit
CTGGCCTCGAGATCTGCCTTCCTCGGCCTCCCAAAGTGCTGGGATTACAG711530.11205159793959737No Hit
CTGCTTTCCTTCTAGACCCTGAGAAATACTTTAGGAAAGAACCAGAGGAA706800.11130671851321437No Hit
TCTCCAGCCTTCCTAGCTTCCCAAAATACAAAGGGAGAAGAATCCTATTG695420.10951459845565865No Hit
GTCTACGGAGATCTCCAGGAGTGAATTTTTGCGGTCTCATGTTCATCATT694860.10942640977092831No Hit
CTGGCCTCGAGATCTGCCTGCCTGGGCCTCCCAAAGTGCTGGGATTACAG693710.1092453080076428No Hit
GACAGGACTAGGTAGGATGATGGGCAGGCAGATATGAGAAGAAGTGATGA690530.10874452226220983No Hit
TCTGTTGGTTGGCGTATGGTTCTTTAGGATGATTGACAGTGGCAGTACCT681880.1073823220427145No Hit
CAAAAGGATCTCAGCAACCCCAAAGAAGTGGAAGAAGAATATCTGAGTCA676380.10651618317482729No Hit
CAAGACTCTTGGATCCATCGATCACAAAGACCAGGTCAATTGGGCCTTCA675630.10639807332920631No Hit
CTGGCCTCGAGATCTGCCTGCTTCAGCCTCCCAAAGTGCTGGGATTACAG663960.10456028413134383No Hit
CTCTCTGGCTAAAGTAGGTGCTCAAATGCTTCTCTCTGGGGCTCTCCAAA658490.10369886965728144No Hit
TGCCAGCTTGTCATCACTTCTTCTCATATCTGCCTGCCCATCATCCTACC651060.10252879478666291No Hit
ACAGTGTCTGGCATCTCCCAGGCTCTGGGCCCACCCAGGGCTTCTCTGGG649550.10229100029747933No Hit
TGATGAAGGTAAGGCTACTGGCTGGCTCCAAAGGGCCTGACCAGCGGCGC644670.10152249890197212No Hit
AGCAGGAGTTCCCACCAGTTTCTTTCTGAGAACATCTGTTCAGGTTTCTC642590.10119494093011662No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)