FastQCFastQC Report
Wed 31 May 2023
EGAF00007912905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007912905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63500210
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAG35805035.638568754339553No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAG18342452.8885652504141324No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAG17170272.7039705852941274No Hit
CTGGCCTCGAGATCTGCCTACCTCGGCCTCCCAAAGTGCTGGGATTACAG9868831.5541413170129672No Hit
CTGGCCTCGAGATCTGCCTACCTCAGCCTCCCAAAGTGCTGGGATTACAG6932571.0917396966088775No Hit
CTGGCCTCGAGATCTGCCTACCTTGGCCTCCCAAAGTGCTGGGATTACAG5349570.8424491824515226No Hit
GTCAGATGATCTTCCCAGCATCAGTACACGAAGTTCAGCCTGCACGTGCC2094660.32986662563793095No Hit
GCCTCACGAGAACAGCTCTCTTGCCCCATGGCCGGACCTCCCCACCCTGG1706040.2686668280309624No Hit
CTGGCCTCGAGATCTGCCTGTCTCGGCCTCCCAAAGTGCTGGGATTACAG1542300.2428810865349894No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGTTGGGATTACAG1502860.23667008345326732No Hit
CTGGCCTCGAGATCTGCCTGCCTCTGCCTCCCAAAGTGCTGGGATTACAG1408510.22181186487414767No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTAGGATTACAG1361210.21436307061031765No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTATAG1340470.2110969396794121No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTATAG1215530.19142141419689793No Hit
TGTAAAACTCTTATTGCTGGACAGAAATCTGCTCGTCTTGATCTACTTTA1208830.19036629957601717No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGAGATTACAG1181000.18598363690450787No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAA1106870.1743096597633299No Hit
GCACGTGCAGGCTGAACTTCGTGTACTGATGCTGGGAAGATCATCTGACC1083490.1706277821758385No Hit
CTGGCCTCGAGATCTGCCTGCCTTAGCCTCCCAAAGTGCTGGGATTACAG1008950.15888923831905438No Hit
AGAATCATCTTCAGACAGCCCACTCGCAATATCAGGAGGAGAATCTTCCT992590.15631286888657533No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAGAGTGCTGGGATTACAG976580.15379161738205274No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCTTCCCAAAGTGCTGGGATTACAG966240.15216327631042478No Hit
CTGGCCTCGAGATCTGCCTGTCTTGGCCTCCCAAAGTGCTGGGATTACAG963920.15179792318797056No Hit
GAGATTCCGGCCTCTGCAACCGTGGGGCATGCGGTGGAGGGGTGGCCCCT950840.14973808748034062No Hit
CCACTGGTGTTGGCGGTATTGGGCGCCAGGGTGGGGAGGTCCGGCCATGG913540.14386409115812374No Hit
CTGGCCTCGAGATCTGCCTGCCTCGACCTCCCAAAGTGCTGGGATTACAG894330.14083890431228496No Hit
GCAGGCGATAAAGTAGATCAAGACGAGCAGATTTCTGTCCAGCAATAAGA873930.13762631651139423No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGAGATTACAG862040.13575388175881622No Hit
CTGAAGGCCCAATTGACCTGGTCTTTGTGATCGATGGATCCAAGAGTCTT858720.1352310488422007No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGTCTCCCAAAGTGCTGGGATTACAG858380.13517750571218584No Hit
GCGGTAGAAGGTCCTAAGGTCAGAAAGACAAGAGGGAATCATCCAAAAGA844730.13302790652188395No Hit
CTGGCCTCGAGATCTGCCTGCTTCGGCCTCCCAAAGTGCTGGGATTACAG842670.13270349814591165No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTTCCAAAGTGCTGGGATTACAG809900.1275428852912455No Hit
CTGGCCTCGAGATCTGCCTGCTTTGGCCTCCCAAAGTGCTGGGATTACAG805850.12690509212489218No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGTTGGGATTACAG793220.12491612232463484No Hit
AATGACTGTCTCTATTTTCCCGTGTCTTTCAGTAATGTATCCTGCATATG791300.12461376111984512No Hit
GCACAACTGCCCCTCACAAGAATTCAGCTTCAGTGCAAAATTCATCTGTT788200.12412557375794506No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGTTGGGATTACAG769170.12112873327505531No Hit
GCGGTGAAAGCGAGGAGACAGGCTGAATTTGAACACTTTCAGAGGAGGTA768670.12104999337797465No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAGAGTGCTGGGATTACAG758290.11941535311458026No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAATGCTGGGATTACAG751590.11836023849369948No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCGAAGTGCTGGGATTACAG750990.11826575061720268No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCTCAAAGTGCTGGGATTACAG748280.11783898037502553No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTATAG744320.11721536039014674No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAATGCTGGGATTACAG740260.11657599242585182No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGCTAGGATTACAG733880.11557127133910265No Hit
CTGGCCTCGAGATCTGCCTGCCTAGGCCTCCCAAAGTGCTGGGATTACAG721810.11367049022357564No Hit
ATCTAGGGATGGCAGTGACTGGATGTATTCAAAATACTGGTCTAAGGTTT717310.11296183114984973No Hit
ACTGTCTACGGCCTGGACAAGCAGAGAGTATCCAGACACCTGCCAAGGTA716850.11288939044453554No Hit
CTGGCCTCGAGATCTGCCTGTCTCAGCCTCCCAAAGTGCTGGGATTACAG713240.11232088838761321No Hit
CTGGCCTCGAGATCTGCCTTCCTCGGCCTCCCAAAGTGCTGGGATTACAG710380.11187049617631187No Hit
CTGCTTTCCTTCTAGACCCTGAGAAATACTTTAGGAAAGAACCAGAGGAA704100.11088152306897882No Hit
GTCTACGGAGATCTCCAGGAGTGAATTTTTGCGGTCTCATGTTCATCATT693140.10915554452497085No Hit
CTGGCCTCGAGATCTGCCTGCCTGGGCCTCCCAAAGTGCTGGGATTACAG692220.10901066311434246No Hit
TCTCCAGCCTTCCTAGCTTCCCAAAATACAAAGGGAGAAGAATCCTATTG690610.10875712064574274No Hit
GACAGGACTAGGTAGGATGATGGGCAGGCAGATATGAGAAGAAGTGATGA687730.10830357883855816No Hit
TCTGTTGGTTGGCGTATGGTTCTTTAGGATGATTGACAGTGGCAGTACCT681050.10725161381356062No Hit
CAAAAGGATCTCAGCAACCCCAAAGAAGTGGAAGAAGAATATCTGAGTCA673540.10606894055940919No Hit
CAAGACTCTTGGATCCATCGATCACAAAGACCAGGTCAATTGGGCCTTCA673040.10599020066232852No Hit
CTGGCCTCGAGATCTGCCTGCTTCAGCCTCCCAAAGTGCTGGGATTACAG662700.10436185959070057No Hit
CTCTCTGGCTAAAGTAGGTGCTCAAATGCTTCTCTCTGGGGCTCTCCAAA657700.10357446061989402No Hit
ACAGTGTCTGGCATCTCCCAGGCTCTGGGCCCACCCAGGGCTTCTCTGGG648510.10212722131155157No Hit
TGCCAGCTTGTCATCACTTCTTCTCATATCTGCCTGCCCATCATCCTACC648350.10210202454448576No Hit
TGATGAAGGTAAGGCTACTGGCTGGCTCCAAAGGGCCTGACCAGCGGCGC641910.10108785467008692No Hit
AGCAGGAGTTCCCACCAGTTTCTTTCTGAGAACATCTGTTCAGGTTTCTC641070.10095557164299142No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)