FastQCFastQC Report
Tue 23 May 2023
EGAF00007912907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007912907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47637136
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAG26915035.650010109759747No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAG13784762.893700410536855No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAG12534492.6312434064046166No Hit
CTGGCCTCGAGATCTGCCTACCTCGGCCTCCCAAAGTGCTGGGATTACAG7675371.6112156700604334No Hit
CTGGCCTCGAGATCTGCCTACCTCAGCCTCCCAAAGTGCTGGGATTACAG5318121.1163811359272313No Hit
CTGGCCTCGAGATCTGCCTACCTTGGCCTCCCAAAGTGCTGGGATTACAG4004050.8405312191732097No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTATAG1270870.2667813614991464No Hit
CTGGCCTCGAGATCTGCCTGTCTCGGCCTCCCAAAGTGCTGGGATTACAG1238550.2599967386788324No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGTTGGGATTACAG1119800.23506870774095234No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTAGGATTACAG1045580.21948842600445165No Hit
CTGGCCTCGAGATCTGCCTGCCTCTGCCTCCCAAAGTGCTGGGATTACAG1031560.21654534395182784No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGAGATTACAG923690.1939012454485089No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTATAG914840.1920434511428227No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAA803160.16859955644688632No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAGAGTGCTGGGATTACAG780160.16377139045470746No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCTTCCCAAAGTGCTGGGATTACAG744370.15625834433035604No Hit
CTGGCCTCGAGATCTGCCTGCCTTAGCCTCCCAAAGTGCTGGGATTACAG711910.14944433267356796No Hit
CTGGCCTCGAGATCTGCCTGTCTTGGCCTCCCAAAGTGCTGGGATTACAG692530.14537607802450592No Hit
AGAATCATCTTCAGACAGCCCACTCGCAATATCAGGAGGAGAATCTTCCT682360.14324118897492075No Hit
CTGGCCTCGAGATCTGCCTGCCTCGACCTCCCAAAGTGCTGGGATTACAG662770.1391288510711475No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGAGATTACAG659470.13843611421140012No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGTCTCCCAAAGTGCTGGGATTACAG658470.1382261939508706No Hit
CTGGCCTCGAGATCTGCCTGCTTCGGCCTCCCAAAGTGCTGGGATTACAG658390.13820940033002824No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAGAGTGCTGGGATTACAG633220.1329257073725003No Hit
CTGGCCTCGAGATCTGCCTGCCTAGGCCTCCCAAAGTGCTGGGATTACAG622730.13072364383954568No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCTCAAAGTGCTGGGATTACAG608150.12766300644102532No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTTCCAAAGTGCTGGGATTACAG599480.12584299778223443No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGTTGGGATTACAG592730.12442603602366019No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGTTGGGATTACAG587520.12333235146630142No Hit
GTGATGATGATGTCTTCAGAACAGGACAGCATCTTCAATGGGACGGGATC587060.12323578814645783No Hit
TGTGATCCCGTCCCATTGAAGATGCTGTCCTGTTCTGAAGACATCATCAT583610.12251156324763102No Hit
TGTGATCCCGCCCCATTGAAGATGCTGTCCTGTTCTGAAGACATCATCAT579330.12161310453256467No Hit
CTGGCCTCGAGATCTGCCTGCCTCGGCCTCCCAAAATGCTGGGATTACAG571820.12003660337598801No Hit
CTGGCCTCGAGATCTGCCTGCTTTGGCCTCCCAAAGTGCTGGGATTACAG565320.11867212168254616No Hit
CTGGCCTCGAGATCTGCCTTCCTCGGCCTCCCAAAGTGCTGGGATTACAG558220.1171816878327866No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCGAAGTGCTGGGATTACAG556550.11683112099770229No Hit
CTGGCCTCGAGATCTGCCTGTCTCAGCCTCCCAAAGTGCTGGGATTACAG551220.11571224600907996No Hit
CTGGCCTCGAGATCTGCCTGCTTCAGCCTCCCAAAGTGCTGGGATTACAG547320.11489355699301487No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGCTAGGATTACAG540500.11346190081620357No Hit
CTTTCACAGTTACTCAGGCCGAAGAATTCCTGGAAGGTGTTCAGGAGAAT539850.11332545264685938No Hit
CTGGCCTCGAGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTATAG536940.11271458468871849No Hit
GCGGTGAAAGCGAGGAGACAGGCTGAATTTGAACACTTTCAGAGGAGGTA536640.11265160861055963No Hit
CTGAAGGCCCAATTGACCTGGTCTTTGTGATCGATGGATCCAAGAGTCTT512860.10765970481516772No Hit
CTGGCCTCGAGATCTGCCTGCCTGGGCCTCCCAAAGTGCTGGGATTACAG510720.10721047545763455No Hit
TCTGTTGGTTGGCGTATGGTTCTTTAGGATGATTGACAGTGGCAGTACCT502720.10553111337339843No Hit
AATGACTGTCTCTATTTTCCCGTGTCTTTCAGTAATGTATCCTGCATATG502240.10543035164834427No Hit
ACTGTCTACGGCCTGGACAAGCAGAGAGTATCCAGACACCTGCCAAGGTA500770.10512176886536588No Hit
ATCTAGGGATGGCAGTGACTGGATGTATTCAAAATACTGGTCTAAGGTTT490860.10304145908351836No Hit
GAGAAGCCTCTAGAAGGGATTCTCTCCTCCTCTGCTTGGCACTTCACACA488680.10258383291556403No Hit
GACAGGACTAGGTAGGATGATGGGCAGGCAGATATGAGAAGAAGTGATGA486250.1020737266824773No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGAATTACAG484900.10179033433076247No Hit
GCACAACTGCCCCTCACAAGAATTCAGCTTCAGTGCAAAATTCATCTGTT481620.10110179587622563No Hit
CTGGCCTCGAGATCTGCCTATCTCGGCCTCCCAAAGTGCTGGGATTACAG480980.10096744690948675No Hit
GCGGTAGAAGGTCCTAAGGTCAGAAAGACAAGAGGGAATCATCCAAAAGA480860.1009422564782232No Hit
CTGGCCTCGAGATCTGCCTGCCTCAGCCTCCCAAAGTGCTAGGATTACAG480450.10085618917140611No Hit
GTCATTCAGAAGAACTCTTTTGCCATCTTAAAACTATATCAGAGAAAGAA479150.10058329283271775No Hit
GAATGCTGTCTTCTGATGCGAAGATCTAGCAATAGAGGGAGAGTATCACA478400.1004258526373206No Hit
CAGTCTGTCTATGGTCTCCTGGAGCAGGGAGTGGGATGGAGACAATCAAA477970.1003355869252929No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)