FastQCFastQC Report
Mon 22 May 2023
EGAF00007912944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007912944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37422021
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGGTTCTGCGCCTTCCATGATGAGACAGGCCGTTTGAATGTTGACGG3860901.0317187305303475No Hit
CCGGACGCTCGACGCCATTAATTATGAGTTTCCCAGGTTCTGCGCCTTCC2109430.5636868195867882No Hit
GCGGCTTTAACCGGACGCTCGACGCCATTAATTATGAGTTTCCCAGGTTC1864690.49828682422042353No Hit
CCAGGTTCTGCGCCTTCCATGATGAGACAGGCCGTTTGAATGTTGACGGG1834870.49031825405688273No Hit
AGCGGCTTTAACCGGACGCTCGACGCCATTAATTATGAGTTTCCCAGGTT1609120.4299928109174007No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATATCTCGATCTCGTAT1421010.3797256166362581TruSeq Adapter, Index 15 (97% over 38bp)
GTCAGTGTTTCCTGCGCGTACACGCAAGGTAAACGCGAACAATTCAGCGG1161500.31037874731565135No Hit
CTTTAACCGGACGCTCGACGCCATTAATTATGAGTTTCCCAGGTTCTGCG1116050.29823349198590854No Hit
GGACGCTCGACGCCATTAATTATGAGTTTCCCAGGTTCTGCGCCTTCCAT979790.26182177600723383No Hit
CTCGACGCCATTAATTATGAGTTTCCCAGGTTCTGCGCCTTCCATGATGA926430.24756279197213857No Hit
CGCAAGGTAAACGCGAACAATTCAGCGGCTTTAACCGGACGCTCGACGCC741360.1981079536030403No Hit
CGGACGCTCGACGCCATTAATTATGAGTTTCCCAGGTTCTGCGCCTTCCA683300.18259302457235008No Hit
GCGAACAATTCAGCGGCTTTAACCGGACGCTCGACGCCATTAATTATGAG647090.17291690365947898No Hit
CGCGAACAATTCAGCGGCTTTAACCGGACGCTCGACGCCATTAATTATGA608850.16269832139744672No Hit
ACCGGACGCTCGACGCCATTAATTATGAGTTTCCCAGGTTCTGCGCCTTC605060.16168554873078608No Hit
ATTAATTATGAGTTTCCCAGGTTCTGCGCCTTCCATGATGAGACAGGCCG605020.16167485983720656No Hit
GCCGTTTGAATGTTGACGGGATGAACATAATAAGCAATGACGGCAGCAAT543560.14525137485225612No Hit
CGTCAGTGTTTCCTGCGCGTACACGCAAGGTAAACGCGAACAATTCAGCG541480.14469555238612047No Hit
GCCATTAATTATGAGTTTCCCAGGTTCTGCGCCTTCCATGATGAGACAGG520980.13921749442661047No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATATCTCGATCGCGTAT476580.12735282255333027TruSeq Adapter, Index 15 (97% over 38bp)
CGGCTTTAACCGGACGCTCGACGCCATTAATTATGAGTTTCCCAGGTTCT469690.12551166063425595No Hit
GGCCGTTTGAATGTTGACGGGATGAACATAATAAGCAATGACGGCAGCAA458120.12241989816637643No Hit
GTCAGTAAGAACGTCAGTGTTTCCTGCGCGTACACGCAAGGTAAACGCGA443640.11855051869058596No Hit
TTTAACCGGACGCTCGACGCCATTAATTATGAGTTTCCCAGGTTCTGCGC421220.11255939383925843No Hit
GTTTTCTTCTGCGTCAGTAAGAACGTCAGTGTTTCCTGCGCGTACACGCA408380.10912825900022878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)