FastQCFastQC Report
Wed 31 May 2023
EGAF00007912946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007912946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37458548
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGGTTCTGCGCCTTCCATGATGAGACAGGCCGTTTGAATGTTGACGG3877121.0350427891652394No Hit
CCGGACGCTCGACGCCATTAATTATGAGTTTCCCAGGTTCTGCGCCTTCC2125580.5674485834314773No Hit
GCGGCTTTAACCGGACGCTCGACGCCATTAATTATGAGTTTCCCAGGTTC1878470.5014796622656063No Hit
CCAGGTTCTGCGCCTTCCATGATGAGACAGGCCGTTTGAATGTTGACGGG1838080.4906970766725928No Hit
AGCGGCTTTAACCGGACGCTCGACGCCATTAATTATGAGTTTCCCAGGTT1625920.4340584691109757No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATATCTCGATCTCGTAT1490080.3977943832740126TruSeq Adapter, Index 15 (97% over 38bp)
GTCAGTGTTTCCTGCGCGTACACGCAAGGTAAACGCGAACAATTCAGCGG1166120.3113094506492884No Hit
CTTTAACCGGACGCTCGACGCCATTAATTATGAGTTTCCCAGGTTCTGCG1122430.29964589123956437No Hit
GGACGCTCGACGCCATTAATTATGAGTTTCCCAGGTTCTGCGCCTTCCAT982170.2622018344117343No Hit
CTCGACGCCATTAATTATGAGTTTCCCAGGTTCTGCGCCTTCCATGATGA929650.24818100263790258No Hit
CGCAAGGTAAACGCGAACAATTCAGCGGCTTTAACCGGACGCTCGACGCC740660.19772789911664487No Hit
CGGACGCTCGACGCCATTAATTATGAGTTTCCCAGGTTCTGCGCCTTCCA688890.1839072886647929No Hit
GCGAACAATTCAGCGGCTTTAACCGGACGCTCGACGCCATTAATTATGAG649390.17336229904052874No Hit
ATTAATTATGAGTTTCCCAGGTTCTGCGCCTTCCATGATGAGACAGGCCG608260.16238216174316206No Hit
CGCGAACAATTCAGCGGCTTTAACCGGACGCTCGACGCCATTAATTATGA604410.16135435895699962No Hit
ACCGGACGCTCGACGCCATTAATTATGAGTTTCCCAGGTTCTGCGCCTTC604380.16134635010412043No Hit
GCCGTTTGAATGTTGACGGGATGAACATAATAAGCAATGACGGCAGCAAT546720.1459533348703212No Hit
CGTCAGTGTTTCCTGCGCGTACACGCAAGGTAAACGCGAACAATTCAGCG541550.14457314255747447No Hit
GCCATTAATTATGAGTTTCCCAGGTTCTGCGCCTTCCATGATGAGACAGG523000.1396210018605099No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATATCTCGATCGCGTAT473170.12631829722817872TruSeq Adapter, Index 15 (97% over 38bp)
CGGCTTTAACCGGACGCTCGACGCCATTAATTATGAGTTTCCCAGGTTCT472130.12604065699503356No Hit
GGCCGTTTGAATGTTGACGGGATGAACATAATAAGCAATGACGGCAGCAA468770.1251436654725645No Hit
GTCAGTAAGAACGTCAGTGTTTCCTGCGCGTACACGCAAGGTAAACGCGA448540.11974302901436543No Hit
TTTAACCGGACGCTCGACGCCATTAATTATGAGTTTCCCAGGTTCTGCGC420830.11234551857162214No Hit
GTTTTCTTCTGCGTCAGTAAGAACGTCAGTGTTTCCTGCGCGTACACGCA405890.10835710983778656No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)