FastQCFastQC Report
Mon 22 May 2023
EGAF00007912962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007912962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36527785
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGTGGTATCTCGTAT7200481.97123367869144TruSeq Adapter, Index 3 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGTGGTATCTCGTATG1289210.3529395499891384TruSeq Adapter, Index 3 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200470.3286457144882998No Hit
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT637380.17449182861758522No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA513040.14045198743915077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)