FastQCFastQC Report
Mon 22 May 2023
EGAF00007912964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007912964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38676280
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGTGGTATCTCGTAT7115441.8397426019255214TruSeq Adapter, Index 3 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1330830.34409462337122393No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGTGGTATCTCGTATG1264740.3270066304205058TruSeq Adapter, Index 3 (97% over 36bp)
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT665130.17197362310956482No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA538830.13931794888236407No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)