FastQCFastQC Report
Wed 31 May 2023
EGAF00007912967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007912967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33195075
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGCTTAAGGTGTAGATCT6242821.8806464513184562Illumina Single End PCR Primer 1 (97% over 34bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1149590.34631342149400174No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGCTTAAGGTGTAGATCTC1118960.33708614907482515Illumina Single End PCR Primer 1 (96% over 33bp)
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT662430.19955671134949987No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)