FastQCFastQC Report
Sun 19 Mar 2023
EGAF00007914463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007914463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512039581
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC61873461.20837260039864Illumina Single End PCR Primer 1 (96% over 33bp)
AGCCATATGAGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT16007330.3126189965380821No Hit
AGCCATATGAGTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA12194220.23814994880249307No Hit
AGCCATATGAGGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC5165620.10088321668242284No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)