FastQCFastQC Report
Sun 19 Mar 2023
EGAF00007914475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007914475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493830615
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC34854190.7057924102174185Illumina Single End PCR Primer 1 (96% over 31bp)
CTCCACGCAAGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT21084990.4269680606983024No Hit
CTCCACGCAAGTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA18109280.3667103547235523No Hit
CTCCACGCAAGGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC5019820.10165064391562681No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)