FastQCFastQC Report
Wed 8 May 2024
EGAF00007914752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007914752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298300
Total Bases39.9 Mbp
Sequences flagged as poor quality0
Sequence length19-142
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGAGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTAC6810.2282936640965471No Hit
CTTGAAACCCAAGGTACATTTCAGATAACTTAACTTTCAGCATAATTATC6590.22091853838417702No Hit
CATGGCATTAGCAAAGACTCAAAAAATAAAAACTATAATTACTCCTTAAT5340.17901441501843782No Hit
CCCAGTGGAGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAAC4790.16057660073751256No Hit
ACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTGAAA4630.15521287294669794No Hit
GTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCGAGAATATCCA4610.15454240697284613No Hit
CTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGA4590.15387194099899432No Hit
GTCAGCCAGCATTTTCCTGACACCAGGGACCAGGCTGCCTTCCCACTAGC4310.14448541736506873No Hit
GTCCATGTGCCCCTCCTTCTGGCCACCATGCGAAGCCACACTGACGTGCC4200.14079785450888369No Hit
TTTATGGCAAATACACAAAGAAAGCCCTCCCCAGTCCTCATGTACTGGTC4010.13442842775729133No Hit
ACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGG3950.13241702983573583No Hit
GTGGCTTTAGGTCAGCCAGCATTTTCCTGACACCAGGGACCAGGCTGCCT3900.13074086490110626No Hit
CTCATGAAAATGGTCAGAGAAACCTTTATCTGTATCAAAGAATGGTCCTG3900.13074086490110626No Hit
GTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCCAAACT3810.12772376801877305No Hit
CTTGCTTCCTGTAGGAATCCTGAGAAGGGAGAAACACAGTCTGGATTATT3800.12738853503184713No Hit
GTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGC3760.12604760308414348No Hit
CCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTGAAAA3590.12034864230640295No Hit
AATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTATT3570.11967817633255111No Hit
CTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCAAAAAG3550.11900771035869931No Hit
ATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAG3400.1139792155548106No Hit
CCTCCACCGTGCAGCTCATCACGCAGCTCATGCCCTTCGGCTGCCTCCTG3350.11230305062018102No Hit
TTTAAACCCACCTATAATGGTGAATATCTTCAAATGATTTAGTATTATTT3250.1089507207509219No Hit
ATCACAGATTTTGGGCTGGCCAAACTGCTGGGTGCGGAAGAGAAAGAATA3220.10794502179014415No Hit
GTGGAGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAA3210.10760978880321823No Hit
AGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTG3200.10727455581629232No Hit
ATGGCATTAGCAAAGACTCAAAAAATAAAAACTATAATTACTCCTTAATG3200.10727455581629232No Hit
AACAAGATTTACCTCTATTGTTGGATCATATTCGTCCACAAAATGATTCT3190.1069393228293664No Hit
CTCCACAGCCCCAGTGTCCCTCACCTTCGGGGTGCATCGCTGGTAACATC3140.10526315789473684No Hit
CTCCACCGTGCAGCTCATCACGCAGCTCATGCCCTTCGGCTGCCTCCTGG3130.10492792490781093No Hit
TTAAAACAAGATTTACCTCTATTGTTGGATCATATTCGTCCACAAAATGA3110.10425745893395912No Hit
CCTCACAGCAGGGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACC3090.10358699296010727No Hit
CCTCTGGGCTGGGCCGCAGGGCCTCTCATGGTCTGGTGGGGAGCCCAGAG3080.10325175997318137No Hit
CCCTGAATTCGGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAG3080.10325175997318137No Hit
GTACTCATGAAAATGGTCAGAGAAACCTTTATCTGTATCAAAGAATGGTC3070.10291652698625545No Hit
CTTGTGGAGCCTCTTACACCCAGTGGAGAAGCTCCCAACCAAGCTCTCTT3040.1019108280254777No Hit
TACGTATTTTGAAACTCAAGATCGCATTCATGCGTCTTCACCTGGAAGGG3000.10056989607777406No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)